{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T19:48:58Z","timestamp":1791143338430,"version":"4.1.0"},"reference-count":49,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"2","content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":["Anesthesiology"],"published-print":{"date-parts":[[2010,2,1]]},"abstract":"\n Background<\/jats:title>\n High tidal volume (VT) mechanical ventilation was shown to induce organ injury other than lung injury and systemic inflammation in animal models of ventilator-induced lung injury. The authors aimed to explore whether high VT mechanical ventilation per se induces early oxidative stress and inflammation in the diaphragm, limb muscles, and lungs of healthy rats exposed to ventilator-induced lung injury.<\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n Protein carbonylation and nitration, antioxidants (immunoblotting), and inflammation (immunohistochemistry) were evaluated in the diaphragm, gastrocnemius, soleus, tibialis anterior, and lungs of mechanically ventilated healthy rats and in nonventilated control animals (n = 8\/group) for 1 h, using two different strategies (moderate VT [VT = 9 ml\/kg] and high VT [VT = 35 ml\/kg]).<\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n The main findings are summarized as follows: compared with controls, (1) the diaphragms and gastrocnemius of high-VT rats exhibited a decrease in reactive carbonyls, (2) the soleus and tibialis of high- and moderate-VT rodents showed a reduction in reactive carbonyls and malondialdehyde-protein adducts, (3) the lungs of high-VT rats exhibited a significant rise in malondialdehyde-protein adducts, (4) the soleus and tibialis of both high- and moderate-VT rats showed a reduction in protein nitration, (5) the lungs of high- and moderate-VT rats showed a reduction in antioxidant enzyme levels, but not in the muscles, and (6) the diaphragms and gastrocnemius of all groups exhibited very low inflammatory cell counts, whereas the lungs of high-VT rats exhibited a significant increase in inflammatory infiltrates.<\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n Although oxidative stress and inflammation increased in the lungs of rats exposed to high VT, the diaphragm and limb muscles exhibited a decline in oxidative stress markers and very low levels of cellular inflammation.<\/jats:p>\n <\/jats:sec>","DOI":"10.1097\/aln.0b013e3181c38bed","type":"journal-article","created":{"date-parts":[[2010,1,16]],"date-time":"2010-01-16T02:55:16Z","timestamp":1263610516000},"page":"384-394","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":15,"title":["Redox Balance and Cellular Inflammation in the Diaphragm, Limb Muscles, and Lungs of Mechanically Ventilated Rats"],"prefix":"10.1097","volume":"112","author":[{"given":"Judith","family":"Mar\u00edn-Corral","sequence":"first","affiliation":[{"name":"Ph.D. Student, Pulmonology Department-Muscle and Respiratory System Research Unit, Institut Municipal d'Investigaci\u00f3 M\u00e8dica (IMIM)-Hospital del Mar, Parc de Recerca Biom\u00e8dica de Barcelona (PRBB); Department of Medicine, Medical School, Universitat Aut\u00f2noma de Barcelona, Barcelona, Spain."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leticia","family":"Mart\u00ednez-Caro","sequence":"additional","affiliation":[{"name":"Post-doctoral Investigator."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9\u00a0A.","family":"Lorente","sequence":"additional","affiliation":[{"name":"Investigator, Servicio de Medicina Intensiva, Hospital Universitario de Getafe, Getafe, Madrid, Spain; Centro de Investigaci\u00f3n en Red de Enfermedades Respiratorias (CIBERES), Instituto de Salud Carlos III (ISCIII), Bunyola, Majorca, Balearic Islands, Spain."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marta","family":"de Paula","sequence":"additional","affiliation":[{"name":"Post-doctoral Investigator."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lara","family":"Pijuan","sequence":"additional","affiliation":[{"name":"Assistant Professor, Department of Medicine, Medical School, Universitat Aut\u00f2noma de Barcelona; Department of Pathology, IMIM-Hospital del Mar, Barcelona, Spain."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicolas","family":"Nin","sequence":"additional","affiliation":[{"name":"Post-doctoral Investigator."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joaquim","family":"Gea","sequence":"additional","affiliation":[{"name":"Professor."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andr\u00e9s","family":"Esteban","sequence":"additional","affiliation":[{"name":"Investigator, Servicio de Medicina Intensiva, Hospital Universitario de Getafe, Getafe, Madrid, Spain; Centro de Investigaci\u00f3n en Red de Enfermedades Respiratorias (CIBERES), Instituto de Salud Carlos III (ISCIII), Bunyola, Majorca, Balearic Islands, Spain."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Esther","family":"Barreiro","sequence":"additional","affiliation":[{"name":"Associate Professor, Pulmonology Department-Muscle and Respiratory System Research Unit, IMIM-Hospital del Mar, PRBB; CIBERES, ISCIII; Health and Experimental Sciences Department, Universitat Pompeu Fabra, PRBB, Barcelona, Spain."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"R1-23-20250520"},{"key":"R2-23-20250520"},{"key":"R3-23-20250520"},{"key":"R4-23-20250520"},{"key":"R5-23-20250520"},{"key":"R6-23-20250520"},{"key":"R7-23-20250520"},{"key":"R8-23-20250520"},{"key":"R9-23-20250520"},{"key":"R10-23-20250520"},{"key":"R11-23-20250520"},{"key":"R12-23-20250520"},{"key":"R13-23-20250520"},{"key":"R14-23-20250520"},{"key":"R15-23-20250520"},{"key":"R16-23-20250520"},{"key":"R17-23-20250520"},{"key":"R18-23-20250520"},{"key":"R19-23-20250520"},{"key":"R20-23-20250520"},{"key":"R21-23-20250520"},{"key":"R22-23-20250520"},{"key":"R23-23-20250520"},{"key":"R24-23-20250520"},{"key":"R25-23-20250520"},{"key":"R26-23-20250520"},{"key":"R27-23-20250520"},{"key":"R28-23-20250520"},{"key":"R29-23-20250520"},{"key":"R30-23-20250520"},{"key":"R31-23-20250520"},{"key":"R32-23-20250520"},{"key":"R33-23-20250520"},{"key":"R34-23-20250520"},{"key":"R35-23-20250520"},{"key":"R36-23-20250520"},{"key":"R37-23-20250520"},{"key":"R38-23-20250520"},{"key":"R39-23-20250520"},{"key":"R40-23-20250520"},{"key":"R41-23-20250520"},{"key":"R42-23-20250520"},{"key":"R43-23-20250520"},{"key":"R44-23-20250520"},{"key":"R45-23-20250520"},{"key":"R46-23-20250520"},{"key":"R47-23-20250520"},{"key":"R48-23-20250520"},{"key":"R49-23-20250520"}],"container-title":["Anesthesiology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/pubs.asahq.org\/anesthesiology\/article-pdf\/112\/2\/384\/249250\/0000542-201002000-00023.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/www.ovid.com\/00000542-201002000-00021","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,6]],"date-time":"2026-08-06T15:58:51Z","timestamp":1786031931000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ovid.com\/00000542-201002000-00021"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,2,1]]},"references-count":49,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2010]]}},"URL":"https:\/\/doi.org\/10.1097\/aln.0b013e3181c38bed","relation":{},"ISSN":["0003-3022"],"issn-type":[{"value":"0003-3022","type":"print"}],"subject":[],"published":{"date-parts":[[2010,2,1]]}}}