{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T20:03:50Z","timestamp":1782936230661,"version":"3.54.5"},"reference-count":38,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"5","content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":["Anesthesiology"],"published-print":{"date-parts":[[2010,5,1]]},"abstract":"<jats:sec>\n            <jats:title>Background<\/jats:title>\n            <jats:p>Neural glial signaling in the spinal cord may underlie pain and sensitization after peripheral injury. The authors test the role of a glial activator, the chemokine (C-C motif) ligand 2 (CCL2), on mechanical hypersensitivity after plantar incision in a rat model of postoperative pain.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Methods<\/jats:title>\n            <jats:p>Twenty-four hours after hind paw incision, rats were intrathecally administered an anti-CCL2 neutralizing antibody (3 and 10 microg) or control immunoglobulin G (10 microg). Mechanical hypersensitivity was assessed acutely and for several days after administration of anti-CCL2 antibody using von Frey filaments. Immunohistochemical analysis was conducted on spinal cord sections to examine the effects of treatment on measures of microglial activation, including levels of ionized calcium-binding adaptor molecule 1 and phosphorylated p38 mitogen-activated protein kinase.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>Neutralization of spinal CCL2 acutely reversed mechanical hypersensitivity within 30 min in a dose-dependent manner. A single administration also produced a sustained decrease in mechanical hypersensitivity 48 and 72 h after incision. Anti-CCL2 antibody reduced microglial activation as measured by the levels of ionized calcium-binding adaptor molecule 1 immunoreactivity and the number of microglia containing phosphorylated p38 mitogen-activated protein kinase 48 h after incision but not within 30 min of administration.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusions<\/jats:title>\n            <jats:p>These results provide evidence that CCL2 contributes to the maintenance of mechanical hypersensitivity after plantar incision and establish a role for neural glial signaling in postoperative pain. The long-term effects of anti-CCL2 treatment correlate with reduced microglial activation. Spinal blockade of CCL2 may serve as a useful therapy for the treatment of certain aspects of postoperative pain.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1097\/aln.0b013e3181d3d978","type":"journal-article","created":{"date-parts":[[2010,4,14]],"date-time":"2010-04-14T03:09:25Z","timestamp":1271214565000},"page":"1250-1258","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":43,"title":["Contribution of the Chemokine (C-C Motif) Ligand 2 (CCL2) to Mechanical Hypersensitivity after Surgical Incision in Rats"],"prefix":"10.1097","volume":"112","author":[{"given":"Christopher M.","family":"Peters","sequence":"first","affiliation":[{"name":"Postdoctoral Research Fellow, Department of Anesthesiology."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James C.","family":"Eisenach","sequence":"additional","affiliation":[{"name":"FM James, III Professor of Anesthesiology and Physiology and Pharmacology, Department of Anesthesiology and Physiology and Pharmacology, Wake Forest University School of Medicine, Winston-Salem, North Carolina."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"R1-33-20251013"},{"key":"R2-33-20251013"},{"key":"R3-33-20251013"},{"key":"R4-33-20251013"},{"key":"R5-33-20251013"},{"key":"R6-33-20251013"},{"key":"R7-33-20251013"},{"key":"R8-33-20251013"},{"key":"R9-33-20251013"},{"key":"R10-33-20251013"},{"key":"R11-33-20251013"},{"key":"R12-33-20251013"},{"key":"R13-33-20251013"},{"key":"R14-33-20251013"},{"key":"R15-33-20251013"},{"key":"R16-33-20251013"},{"key":"R17-33-20251013"},{"key":"R18-33-20251013"},{"key":"R19-33-20251013"},{"key":"R20-33-20251013"},{"key":"R21-33-20251013"},{"key":"R22-33-20251013"},{"key":"R23-33-20251013"},{"key":"R24-33-20251013"},{"key":"R25-33-20251013"},{"key":"R26-33-20251013"},{"key":"R27-33-20251013"},{"key":"R28-33-20251013"},{"key":"R29-33-20251013"},{"key":"R30-33-20251013"},{"key":"R31-33-20251013"},{"key":"R32-33-20251013"},{"key":"R33-33-20251013"},{"key":"R34-33-20251013"},{"key":"R35-33-20251013"},{"key":"R36-33-20251013"},{"key":"R37-33-20251013"},{"key":"R38-33-20251013"}],"container-title":["Anesthesiology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/pubs.asahq.org\/anesthesiology\/article-pdf\/112\/5\/1250\/251077\/0000542-201005000-00033.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/journals.lww.com\/00000542-201005000-00031","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,13]],"date-time":"2025-10-13T16:13:11Z","timestamp":1760371991000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.lww.com\/00000542-201005000-00031"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,5,1]]},"references-count":38,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2010]]}},"URL":"https:\/\/doi.org\/10.1097\/aln.0b013e3181d3d978","relation":{},"ISSN":["0003-3022"],"issn-type":[{"value":"0003-3022","type":"print"}],"subject":[],"published":{"date-parts":[[2010,5,1]]}}}