{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T06:32:39Z","timestamp":1792737159314,"version":"4.5.0"},"reference-count":32,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"4","content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":["Anesthesiology"],"published-print":{"date-parts":[[2010,10,1]]},"abstract":"<jats:sec>\n            <jats:title>Background<\/jats:title>\n            <jats:p>Spinal cord ischemia and paralysis are devastating perioperative complications that can accompany open or endovascular repair surgery for aortic aneurysms. Here, we report on the development of a new mouse model of spinal cord ischemia with delayed paralysis induced by cross-clamping the descending aorta.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Methods<\/jats:title>\n            <jats:p>Transient aortic occlusion was produced in mice by cross-clamping the descending aorta through a lateral thoracotomy. To establish an optimal surgical procedure with limited mortality, variable cross-clamp times and core temperatures were tested between experiments.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>The onset of paresis or paralysis and postsurgical mortality varied as a function of cross-clamp time and core temperature that was maintained during the period of cross-clamp. Using optimal surgical parameters (7.5-min cross-clamp duration at 33\u00b0C core temperature), the onset of paralysis is delayed 24-36 h after reperfusion, and more than 95% of mice survive through 9 weeks after surgery. These mice are further stratified into two groups, 70% (n = 19\/27) of mice developing severe hind limb paralysis and the remaining mice showing mild, though still permanent, behavioral deficits.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusion<\/jats:title>\n            <jats:p>This new model should prove useful as a preclinical tool for screening neuroprotective therapeutics and for defining the basic biologic mechanisms that cause delayed paralysis and neurodegeneration after transient spinal cord ischemia.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1097\/aln.0b013e3181ec61ee","type":"journal-article","created":{"date-parts":[[2010,8,31]],"date-time":"2010-08-31T11:41:30Z","timestamp":1283254890000},"page":"880-891","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":47,"title":["A Mouse Model of Ischemic Spinal Cord Injury with Delayed Paralysis Caused by Aortic Cross-clamping"],"prefix":"10.1097","volume":"113","author":[{"given":"Hamdy","family":"Awad","sequence":"first","affiliation":[{"name":"Assistant Professor, Department of Anesthesiology."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel P.","family":"Ankeny","sequence":"additional","affiliation":[{"name":"Research Scientist."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhen","family":"Guan","sequence":"additional","affiliation":[{"name":"Research Associate."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ping","family":"Wei","sequence":"additional","affiliation":[{"name":"Research Associate."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dana M.","family":"McTigue","sequence":"additional","affiliation":[{"name":"Associate Professor."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Phillip G.","family":"Popovich","sequence":"additional","affiliation":[{"name":"Professor, Department of Neuroscience, Center for Brain and Spinal Cord Repair, The Ohio State University College of Medicine, Columbus, Ohio."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"R1-20-20250520"},{"key":"R2-20-20250520"},{"key":"R3-20-20250520"},{"key":"R4-20-20250520"},{"key":"R5-20-20250520"},{"key":"R6-20-20250520"},{"key":"R7-20-20250520"},{"key":"R8-20-20250520"},{"key":"R9-20-20250520"},{"key":"R10-20-20250520"},{"key":"R11-20-20250520"},{"key":"R12-20-20250520"},{"key":"R13-20-20250520"},{"key":"R14-20-20250520"},{"key":"R15-20-20250520"},{"key":"R16-20-20250520"},{"key":"R17-20-20250520"},{"key":"R18-20-20250520"},{"key":"R19-20-20250520"},{"key":"R20-20-20250520"},{"key":"R21-20-20250520"},{"key":"R22-20-20250520"},{"key":"R23-20-20250520"},{"key":"R24-20-20250520"},{"key":"R25-20-20250520"},{"key":"R26-20-20250520"},{"key":"R27-20-20250520"},{"key":"R28-20-20250520"},{"key":"R29-20-20250520"},{"key":"R30-20-20250520"},{"key":"R31-20-20250520"},{"key":"R32-20-20250520"}],"container-title":["Anesthesiology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/pubs.asahq.org\/anesthesiology\/article-pdf\/113\/4\/880\/251846\/0000542-201010000-00020.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/journals.lww.com\/00000542-201010000-00018","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,5,20]],"date-time":"2025-05-20T10:20:57Z","timestamp":1747736457000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.lww.com\/00000542-201010000-00018"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,10,1]]},"references-count":32,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2010]]}},"URL":"https:\/\/doi.org\/10.1097\/aln.0b013e3181ec61ee","relation":{},"ISSN":["0003-3022"],"issn-type":[{"value":"0003-3022","type":"print"}],"subject":[],"published":{"date-parts":[[2010,10,1]]}}}