{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,21]],"date-time":"2025-05-21T04:09:04Z","timestamp":1747800544385,"version":"3.41.0"},"reference-count":38,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"4","content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":["Anesthesiology"],"published-print":{"date-parts":[[2010,10,1]]},"abstract":"<jats:sec>\n            <jats:title>Background<\/jats:title>\n            <jats:p>Malignant hyperthermia susceptibility (MHS) is diagnosed by an invasive in vitro caffeine-halothane contracture test (CHCT) carried out on biopsied skeletal muscle tissue. We are presenting a novel blood test approach for malignant hyperthermia testing in a swine model. Our main aim was to determine whether adenosine production from lymphocytes after 4-chloro-m-cresol (4CmC) stimulation distinguishes homozygous swine carrying the Arg615Cys mutation in the ryanodine receptor type 1 (RyR1) gene (MHS swine) from normal swine.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Methods<\/jats:title>\n            <jats:p>Lymphocytes were isolated from arterial blood (40 ml) obtained from MHS (n = 7) and normal (n = 7) swine. Cells were suspended in Hank's balanced salt solution and treated with 4CmC (0-10 mm) at 37\u00b0C in the presence of adenosine deaminase inhibitor. After termination and purification of samples, aliquots (50 \u03bcl) were assayed for adenosine content using high performance liquid chromatography.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>Baseline adenosine levels before stimulating lymphocytes with 4CmC were 0.025 \u00b1 0.004 and 0.041 \u00b1 0.006 \u03bcm (mean \u00b1 SEM) in lymphocytes from normal and MHS swine, respectively (P = 0.125). Maximum responses were achieved at 1 mm 4CmC for both cell-line groups. Adenosine levels after stimulation with 4CmC (1 mm) were 0.185 \u00b1 0.009 and 0.397 \u00b1 0.038 \u03bcm in lymphocytes from normal and MHS swine, respectively (P = 0.0035). There was no overlap between adenosine levels in stimulated lymphocytes from MHS and normal swine.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusion<\/jats:title>\n            <jats:p>4CmC stimulation of porcine lymphocytes induces increased adenosine formation in MHS cells relative to those from normal swine; evaluation of adenosine formation in response to RyR1 agonists in human lymphocytes is needed.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1097\/aln.0b013e3181ee2cd0","type":"journal-article","created":{"date-parts":[[2010,9,4]],"date-time":"2010-09-04T08:22:36Z","timestamp":1283588556000},"page":"917-924","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":7,"title":["Lymphocyte-based Determination of Susceptibility to Malignant Hyperthermia: A Pilot Study in Swine"],"prefix":"10.1097","volume":"113","author":[{"given":"Saiid","family":"Bina","sequence":"first","affiliation":[{"name":"Research Assistant Professor."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John","family":"Capacchione","sequence":"additional","affiliation":[{"name":"Research Assistant Professor."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sheila","family":"Muldoon","sequence":"additional","affiliation":[{"name":"Professor."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Munkhuu","family":"Bayarsaikhan","sequence":"additional","affiliation":[{"name":"Postdoctoral Associate, Department of Anesthesiology."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rolf","family":"Bunger","sequence":"additional","affiliation":[{"name":"Professor, Department of Anatomy, Physiology, and Genetics, Uniformed Services University of the Health Sciences, Bethesda, Maryland."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"276","reference":[{"key":"R1-23-20250520"},{"key":"R2-23-20250520"},{"key":"R3-23-20250520"},{"key":"R4-23-20250520"},{"key":"R5-23-20250520"},{"key":"R6-23-20250520"},{"key":"R7-23-20250520"},{"key":"R8-23-20250520"},{"key":"R9-23-20250520"},{"key":"R10-23-20250520"},{"key":"R11-23-20250520"},{"key":"R12-23-20250520"},{"key":"R13-23-20250520"},{"key":"R14-23-20250520"},{"key":"R15-23-20250520"},{"key":"R16-23-20250520"},{"key":"R17-23-20250520"},{"key":"R18-23-20250520"},{"key":"R19-23-20250520"},{"key":"R20-23-20250520"},{"key":"R21-23-20250520"},{"key":"R22-23-20250520"},{"key":"R23-23-20250520"},{"key":"R24-23-20250520"},{"key":"R25-23-20250520"},{"key":"R26-23-20250520"},{"key":"R27-23-20250520"},{"key":"R28-23-20250520"},{"key":"R29-23-20250520"},{"key":"R30-23-20250520"},{"key":"R31-23-20250520"},{"key":"R32-23-20250520"},{"key":"R33-23-20250520"},{"key":"R34-23-20250520"},{"key":"R35-23-20250520"},{"key":"R36-23-20250520"},{"key":"R37-23-20250520"},{"key":"R38-23-20250520"}],"container-title":["Anesthesiology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/pubs.asahq.org\/anesthesiology\/article-pdf\/113\/4\/917\/252174\/0000542-201010000-00023.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/journals.lww.com\/00000542-201010000-00021","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,5,20]],"date-time":"2025-05-20T10:20:27Z","timestamp":1747736427000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.lww.com\/00000542-201010000-00021"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,10,1]]},"references-count":38,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2010]]}},"URL":"https:\/\/doi.org\/10.1097\/aln.0b013e3181ee2cd0","relation":{},"ISSN":["0003-3022"],"issn-type":[{"type":"print","value":"0003-3022"}],"subject":[],"published":{"date-parts":[[2010,10,1]]}}}