{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T13:05:05Z","timestamp":1791205505345,"version":"4.1.0"},"reference-count":0,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"2","content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":["Anesthesiology"],"published-print":{"date-parts":[[2012,2,1]]},"abstract":"<jats:sec>\n            <jats:title>Background<\/jats:title>\n            <jats:p>Kinins (e.g., bradykinin) acting through the constitutively expressed B2 and the injury-induced B1 receptors are involved in pain and hyperalgesia, as previously shown by use of receptor-selective antagonists and single-receptor knockout models. Because the overall contribution of kinins to painful processes remains unclear, the aim of this study was to analyze pain-related behaviors of mice unable to respond to kinins because of a lack of both B1 and B2 receptors.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Methods<\/jats:title>\n            <jats:p>In knockout mice lacking both B1 and B2 receptors and in wild-type mice (n = 8-21 per group) the authors assessed nociceptive thresholds to mechanical and heat stimuli (von Frey and Hargreaves tests, respectively) in healthy animals and after induction of inflammatory and neuropathic pain, acid-induced visceral nociception, and modulation of nociceptive responses by peripherally administered opioid agonists.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>In knockout mice lacking both B1 and B2 receptors baseline nociceptive responses to heat were unaltered, nocifensive responses to bradykinin were abolished, acute acetic acid-induced visceral nociception was reduced by approximately 70% (mean difference: 19.5 writhes\/30 min) and heat hypersensitivity in carrageenan-induced paw inflammation was decreased 48 h after injection (mean difference 2.88 s), hypersensitivities in chronic complete Freund's adjuvant-induced paw inflammation or after chronic constriction injury of the sciatic nerve were unchanged, and peripheral \u03bc- and \u03b4-opioid-induced analgesia after chronic constriction injury was reduced by 30-35% (mean differences: \u03bc-agonist: 0.495 g, \u03b4-agonist: 0.555 g).<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusions<\/jats:title>\n            <jats:p>These data suggest that kinins are important for nociception associated with acute short-lasting inflammation but are less essential in chronic stages of pain. The results also highlight a new protective function of kinins via interactions with the opioid system.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1097\/aln.0b013e318242b2ea","type":"journal-article","created":{"date-parts":[[2012,1,7]],"date-time":"2012-01-07T12:44:23Z","timestamp":1325940263000},"page":"448-457","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":40,"title":["Impaired Nociception and Peripheral Opioid Antinociception in Mice Lacking Both Kinin B1 and B2 Receptors"],"prefix":"10.1097","volume":"116","author":[{"given":"Cecile","family":"Cayla","sequence":"first","affiliation":[{"name":"Principal Scientist."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dominika","family":"Labuz","sequence":"additional","affiliation":[{"name":"Scientist."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Halina","family":"Machelska","sequence":"additional","affiliation":[{"name":"Professor."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Bader","sequence":"additional","affiliation":[{"name":"Professor, Max-Delbr\u00fcck-Center for Molecular Medicine, Berlin, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Sch\u00e4fer","sequence":"additional","affiliation":[{"name":"Current position: Professor, Campus Virchow Klinikum, Charit\u00e9-Universit\u00e4tsmedizin, Berlin, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christoph","family":"Stein","sequence":"additional","affiliation":[{"name":"Chair, Klinik f\u00fcr Anaesthesiologie und Operative Intensivmedizin, Campus Benjamin Franklin, Berlin, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","container-title":["Anesthesiology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/pubs.asahq.org\/anesthesiology\/article-pdf\/116\/2\/448\/256472\/0000542-201202000-00030.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/journals.lww.com\/00000542-201202000-00026","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,5,20]],"date-time":"2025-05-20T11:26:29Z","timestamp":1747740389000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.lww.com\/00000542-201202000-00026"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,2,1]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2012]]}},"URL":"https:\/\/doi.org\/10.1097\/aln.0b013e318242b2ea","relation":{},"ISSN":["0003-3022"],"issn-type":[{"value":"0003-3022","type":"print"}],"subject":[],"published":{"date-parts":[[2012,2,1]]}}}