{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T14:28:47Z","timestamp":1791210527309,"version":"4.1.0"},"reference-count":0,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"3","content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":["Anesthesiology"],"published-print":{"date-parts":[[2012,9,1]]},"abstract":"<jats:sec>\n            <jats:title>Background<\/jats:title>\n            <jats:p>Controlled mechanical ventilation is associated with ventilator-induced diaphragmatic dysfunction, which impedes weaning from mechanical ventilation. To design future clinical trials in humans, a better understanding of the molecular mechanisms using knockout models, which exist only in the mouse, is needed. The aims of this study were to ascertain the feasibility of developing a murine model of ventilator-induced diaphragmatic dysfunction and to determine whether atrophy, sarcolemmal injury, and the main proteolysis systems are activated under these conditions.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Methods<\/jats:title>\n            <jats:p>Healthy adult male C57\/BL6 mice were assigned to three groups: (1) mechanical ventilation with end-expiratory positive pressure of 2-4 cm H2O for 6 h (n=6), (2) spontaneous breathing with continuous positive airway pressure of 2-4 cm H2O for 6 h (n=6), and (3) controls with no specific intervention (n=6). Airway pressure and hemodynamic parameters were monitored. Upon euthanasia, arterial blood gases and isometric contractile properties of the diaphragm and extensor digitorum longus were evaluated. Histology and immunoblotting for the main proteolysis pathways were performed.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>Hemodynamic parameters and arterial blood gases were comparable between groups and within normal physiologic ranges. Diaphragmatic but not extensor digitorum longus force production declined in the mechanical ventilation group (maximal force decreased by approximately 40%) compared with the control and continuous positive airway pressure groups. No histologic difference was found between groups. In opposition with the calpains, caspase 3 was activated in the mechanical ventilation group.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusion<\/jats:title>\n            <jats:p>Controlled mechanical ventilation for 6 h in the mouse is associated with significant diaphragmatic but not limb muscle weakness without atrophy or sarcolemmal injury and activates proteolysis.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1097\/aln.0b013e318261e7f8","type":"journal-article","created":{"date-parts":[[2012,7,4]],"date-time":"2012-07-04T18:38:39Z","timestamp":1341427119000},"page":"560-567","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":65,"title":["Rapid Onset of Specific Diaphragm Weakness in a Healthy Murine Model of Ventilator-induced Diaphragmatic Dysfunction"],"prefix":"10.1097","volume":"117","author":[{"given":"Segolene","family":"Mrozek","sequence":"first","affiliation":[{"name":"Research Fellow."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Boris","family":"Jung","sequence":"additional","affiliation":[{"name":"Associate Professor of Anesthesiology and Intensive Care Medicine, Department of Anesthesiology and Critical Care, Saint Eloi University Hospital, Montpellier, France; Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale U1046, Arnaud de Villeneuve University Hospital; Meakins Christie Laboratories, Department of Medicine, McGill University, Montreal, Canada."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Basil\u00a0J.","family":"Petrof","sequence":"additional","affiliation":[{"name":"Professor of Medicine, Meakins Christie Laboratories, McGill University Health Centre Research Institute, Respiratory Division, Department of Medicine, McGill University."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marion","family":"Pauly","sequence":"additional","affiliation":[{"name":"Ph.D. Student."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephanie","family":"Roberge","sequence":"additional","affiliation":[{"name":"Ph.D. Student."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alain","family":"Lacampagne","sequence":"additional","affiliation":[{"name":"Research Director."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C\u00e9cile","family":"Cassan","sequence":"additional","affiliation":[{"name":"Research Engineer."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jerome","family":"Thireau","sequence":"additional","affiliation":[{"name":"Research Fellow."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicolas","family":"Molinari","sequence":"additional","affiliation":[{"name":"Associate Professor of Medical Biostatistics, Laboratoire de Biostatistique, Institut Universitaire de Recherche Clinique, Montpellier, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emmanuel","family":"Futier","sequence":"additional","affiliation":[{"name":"Staff Anesthesiologist, Department of Anesthesiology and Critical Care, Clermont-Ferrand University Hospital and Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale U 1046, Arnaud de Villeneuve University Hospital, Montpellier, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valerie","family":"Scheuermann","sequence":"additional","affiliation":[{"name":"Research Technician."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean","family":"Michel Constantin","sequence":"additional","affiliation":[{"name":"Professor of Anesthesiology and Intensive Care Medicine, Department of Anesthesiology and Critical Care, Clermont-Ferrand University Hospital."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefan","family":"Matecki","sequence":"additional","affiliation":[{"name":"Professor of Medicine, Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale U1046, Arnaud de Villeneuve University Hospital."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samir","family":"Jaber","sequence":"additional","affiliation":[{"name":"Professor of Anesthesiology and Intensive Care Medicine, Department of Anesthesiology and Critical Care Saint Eloi University Hospital; Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale U1046."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","container-title":["Anesthesiology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/pubs.asahq.org\/anesthesiology\/article-pdf\/117\/3\/560\/257462\/0000542-201209000-00023.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/journals.lww.com\/00000542-201209000-00019","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,13]],"date-time":"2025-10-13T20:10:26Z","timestamp":1760386226000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.lww.com\/00000542-201209000-00019"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,9,1]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2012]]}},"URL":"https:\/\/doi.org\/10.1097\/aln.0b013e318261e7f8","relation":{},"ISSN":["0003-3022"],"issn-type":[{"value":"0003-3022","type":"print"}],"subject":[],"published":{"date-parts":[[2012,9,1]]}}}