{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T18:20:48Z","timestamp":1791829248634,"version":"4.3.4"},"reference-count":22,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"3","content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2013,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background:<\/jats:title><jats:p>Oxytocin receptor desensitization has been shown to occur in humans at biomolecular level and in isolated rat myometrium; however, its effect on human myometrial contractility has not been demonstrated. The objective of this<jats:italic toggle=\"yes\">in vitro<\/jats:italic>study was to investigate the contractile response of human pregnant myometrium to oxytocin after pretreatment with different concentrations of oxytocin for variable durations.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods:<\/jats:title><jats:p>Myometrial samples were obtained from 62 women undergoing elective cesarean deliveries under regional anesthesia. The strips were pretreated with oxytocin 10<jats:sup>\u221210<\/jats:sup>, 10<jats:sup>\u22128<\/jats:sup>, 10<jats:sup>\u22125<\/jats:sup><jats:sc>M,<\/jats:sc>or physiological salt solution (control) for 2, 4, 6, or 12\u2009h, followed by a dose\u2013response testing with oxytocin 10<jats:sup>\u221210<\/jats:sup>to 10<jats:sup>\u22125<\/jats:sup><jats:sc>M<\/jats:sc>. Amplitude and frequency of contractions, motility index, and area under the curve during the dose\u2013response period were recorded, analyzed with linear regression models, and compared among groups.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results:<\/jats:title><jats:p>Pretreatment with oxytocin 10<jats:sup>\u22125<\/jats:sup>and 10<jats:sup>\u22128<\/jats:sup><jats:sc>M<\/jats:sc>significantly reduced motility index (estimate [standard error]: \u22120.771 [0.270] square root units,<jats:italic toggle=\"yes\">P<\/jats:italic>= 0.005 and \u22120.697 [0.293],<jats:italic toggle=\"yes\">P<\/jats:italic>= 0.02, respectively) and area under the curve (\u22123.947 [1.909],<jats:italic toggle=\"yes\">P<\/jats:italic>= 0.04 and \u22124.241 [2.189],<jats:italic toggle=\"yes\">P<\/jats:italic>= 0.05, respectively) compared with control group, whereas pretreatment with oxytocin 10<jats:sup>\u221210<\/jats:sup><jats:sc>M<\/jats:sc>did not significantly attenuate contractions. Increase in duration of oxytocin pretreatment from 2 to 12\u2009h significantly decreased amplitude (type 3 generalized estimating equation analysis: chi-square = 14.0;<jats:italic toggle=\"yes\">df<\/jats:italic>= 3;<jats:italic toggle=\"yes\">P<\/jats:italic>= 0.003), motility index (chi-square = 9.3;<jats:italic toggle=\"yes\">df<\/jats:italic>= 3;<jats:italic toggle=\"yes\">P<\/jats:italic>= 0.03), and area under the curve (chi-square = 10.5;<jats:italic toggle=\"yes\">df<\/jats:italic>= 3;<jats:italic toggle=\"yes\">P<\/jats:italic>= 0.02), but not the frequency of oxytocin-induced contractions.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion:<\/jats:title><jats:p>Pretreatment with oxytocin decreases oxytocin-induced myometrial contractions in a concentration and time-dependent manner, likely as a function of the oxytocin receptor desensitization phenomenon.<\/jats:p><\/jats:sec>","DOI":"10.1097\/aln.0b013e318297d347","type":"journal-article","created":{"date-parts":[[2013,5,14]],"date-time":"2013-05-14T15:03:18Z","timestamp":1368543798000},"page":"552-561","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":70,"title":["Oxytocin Pretreatment Attenuates Oxytocin-induced Contractions in Human Myometrium In Vitro"],"prefix":"10.1097","volume":"119","author":[{"given":"Mrinalini","family":"Balki","sequence":"first","affiliation":[{"name":"Associate Professor"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Magda","family":"Erik-Soussi","sequence":"additional","affiliation":[{"name":"Laboratory Technician, Department of Anesthesia and Pain Management"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John","family":"Kingdom","sequence":"additional","affiliation":[{"name":"Professor, Department of Obstetrics and Gynecology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jose C. 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