{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T10:03:31Z","timestamp":1789898611969,"version":"4.0.1"},"reference-count":32,"publisher":"SAGE Publications","issue":"2","license":[{"start":{"date-parts":[[2014,4,1]],"date-time":"2014-04-01T00:00:00Z","timestamp":1396310400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Dermatitis\u00ae"],"published-print":{"date-parts":[[2014,4,1]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>We previously demonstrated that repeated intradermal steroid injections administered at weekly intervals into positive patch-test sites induce hyposensitization and desensitization.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>\n                      To examine changes in CD4\n                      <jats:sup>+<\/jats:sup>\n                      CD25\n                      <jats:sup>+<\/jats:sup>\n                      CD127lo\/\n                      <jats:sup>\u2212<\/jats:sup>\n                      regulatory T cells during the attenuation of the patch-test response.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Ten patients with known allergic contact dermatitis were patch tested weekly for 10 weeks. The patch-test site was injected intradermally with 2 mg triamcinolone. At weeks 1 and 7, a biopsy was performed on the patch-test site in 6 patients, and flow cytometry was performed assessing CD4\n                      <jats:sup>+<\/jats:sup>\n                      CD25\n                      <jats:sup>+<\/jats:sup>\n                      CD127lo\/\n                      <jats:sup>\u2212<\/jats:sup>\n                      regulatory T cells. Secondary outcomes were clinical score, reaction size, erythema, and temperature. Statistical analysis included regression, correlation, and repeated-measures analysis of variance.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The percentage of CD4\n                      <jats:sup>+<\/jats:sup>\n                      CD25\n                      <jats:sup>+<\/jats:sup>\n                      CD127lo\/\n                      <jats:sup>\u2212<\/jats:sup>\n                      regulatory T cells, measured by flow cytometry, increased from week 1 to week 7 by an average of 19.2%. The average grade of patch-test reaction decreased from +++ (vesicular reaction) to ++ (palpable erythema). The mean drop in temperature following treatment was 0.28\u00b0C per week. The mean area decreased 8.6 mm\n                      <jats:sup>2<\/jats:sup>\n                      \/wk over 10 weeks.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      Intradermal steroid injections of weekly patch-test reactions resulted in hyposensitization of the allergic contact dermatitis reaction. CD4\n                      <jats:sup>+<\/jats:sup>\n                      CD25\n                      <jats:sup>+<\/jats:sup>\n                      CD127lo\/\n                      <jats:sup>\u2212<\/jats:sup>\n                      regulatory T cells showed a tendency to increase; however, further studies are needed to determine if this is significant.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1097\/der.0000000000000022","type":"journal-article","created":{"date-parts":[[2014,4,16]],"date-time":"2014-04-16T12:01:15Z","timestamp":1397649675000},"page":"60-65","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":3,"title":["Flow Cytometric Analysis of Regulatory T Cells During Hyposensitization of Acquired Allergic Contact Dermatitis"],"prefix":"10.1177","volume":"25","author":[{"given":"Kathleen","family":"Fraser","sequence":"first","affiliation":[{"name":"From the Division of Dermatology, Department of Medicine, Royal University Hospital, University of Saskatchewan, Saskatoon, Saskatchewan, Canada."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariam","family":"Abbas","sequence":"additional","affiliation":[{"name":"From the Division of Dermatology, Department of Medicine, Royal University Hospital, University of Saskatchewan, Saskatoon, Saskatchewan, Canada."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter R.","family":"Hull","sequence":"additional","affiliation":[{"name":"From the Division of Dermatology, Department of Medicine, Royal University Hospital, University of Saskatchewan, Saskatoon, Saskatchewan, Canada."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2014,4,1]]},"reference":[{"key":"e_1_3_1_2_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.193.11.1285"},{"key":"e_1_3_1_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/ni904"},{"key":"e_1_3_1_4_2","doi-asserted-by":"crossref","first-page":"471","DOI":"10.1189\/jlb.0503228","article-title":"The role of the combination of IL-2 and TGF-beta or IL-10 in the generation and function of CD4++ CD25+ and CD8+ regulatory T cell subsets","volume":"74","author":"Horwitz DA","year":"2003","unstructured":"Horwitz DA, , Zheng SG, , Gray JD. 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