{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T15:00:00Z","timestamp":1784818800949,"version":"3.55.0"},"reference-count":36,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"6","content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2022,8]]},"abstract":"<jats:sec>\n            <jats:title>Objectives<\/jats:title>\n            <jats:p>This study explores the potential of gene polymorphisms in the canonical and noncanonical NF-kB signaling pathway as a prediction biomarker of anti-tumor necrosis factor (TNF)\u03b1 response in Crohn\u2019s patients.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Materials and methods<\/jats:title>\n            <jats:p>A total of 109 Greek patients with Crohn\u2019s disease (CD) were recruited, and the genotype of <jats:italic toggle=\"yes\">TLR2<\/jats:italic> rs3804099, <jats:italic toggle=\"yes\">LTA<\/jats:italic> rs909253, <jats:italic toggle=\"yes\">TLR4<\/jats:italic> rs5030728, and <jats:italic toggle=\"yes\">MAP3K14\/NIK<\/jats:italic> rs7222094 single nucleotide polymorphisms was investigated for association with response to anti-TNF\u03b1 therapy. Patient\u2019s response to therapy was based on the Crohn\u2019s Disease Activity Index, depicting the maximum response within 24\u2009months after initiation of treatment.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>Seventy-three patients (66.7%) were classified as responders while 36 as nonresponders (33.3%). Comparing allelic frequencies between responders and nonresponders, the presence of <jats:italic toggle=\"yes\">TLR2<\/jats:italic> rs3804099 T allele was associated with nonresponse (<jats:italic toggle=\"yes\">P<\/jats:italic>\u2009=\u20090.003), even after stratification by anti-TNF\u03b1 drugs (infliximab: <jats:italic toggle=\"yes\">P<\/jats:italic>\u2009=\u20090.032, adalimumab: <jats:italic toggle=\"yes\">P<\/jats:italic>\u2009=\u20090.026). No other association was identified for the rest of the polymorphisms under study. Haplotype analysis further enhanced the association of rs3804099 T allele with loss of response, even though the results were NS (<jats:italic toggle=\"yes\">P<\/jats:italic>\u2009=\u20090.073).<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusion<\/jats:title>\n            <jats:p>Our results suggest that polymorphisms in the canonical NF-kB pathway genes could potentially act as a predictive biomarker of anti-TNF\u03b1 response in CD.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1097\/fpc.0000000000000471","type":"journal-article","created":{"date-parts":[[2022,7,19]],"date-time":"2022-07-19T12:09:51Z","timestamp":1658232591000},"page":"235-241","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":9,"title":["Pharmacogenetic analysis of canonical versus noncanonical pathway of NF-kB in Crohn\u2019s disease patients under anti-tumor necrosis factor-\u03b1 treatment"],"prefix":"10.1097","volume":"32","author":[{"given":"Eleana F.","family":"Stavrou","sequence":"first","affiliation":[{"name":"Laboratory of Genetics, Department of Biology, University of Patras, Patras"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fani","family":"Chatzopoulou","sequence":"additional","affiliation":[{"name":"Laboratory of Pharmacology, School of Pharmacy, Aristotle University of Thessaloniki, Thessaloniki"},{"name":"Labnet Laboratories, Department of Molecular Biology and Genetics"},{"name":"Laboratory of Microbiology, School of Medicine, Aristotle University of Thessaloniki, Thessaloniki"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charalabos","family":"Antonatos","sequence":"additional","affiliation":[{"name":"Laboratory of Genetics, Department of Biology, University of Patras, Patras"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Panagiota","family":"Pappa","sequence":"additional","affiliation":[{"name":"Laboratory of Genetics, Department of Biology, University of Patras, Patras"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eutychia","family":"Makridou","sequence":"additional","affiliation":[{"name":"Laboratory of Genetics, Department of Biology, University of Patras, Patras"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Konstantinos","family":"Oikonomou","sequence":"additional","affiliation":[{"name":"Gastroenterology Department, University General Hospital of Larissa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andreas","family":"Kapsoritakis","sequence":"additional","affiliation":[{"name":"Gastroenterology Department, University General Hospital of Larissa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Petros S.","family":"Potamianos","sequence":"additional","affiliation":[{"name":"Gastroenterology Department, University General Hospital of Larissa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Konstantinos","family":"Karmiris","sequence":"additional","affiliation":[{"name":"Gastroenterology Department, \u201cVenizeleio Pananeio\u201d General Hospital of Heraklion, Crete"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charalambos","family":"Tzathas","sequence":"additional","affiliation":[{"name":"Gastroenterology Department, \u201cTzaneio\u201d General Hospital of Piraeus, Piraeus, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dimitris","family":"Chatzidimitriou","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology, School of Medicine, Aristotle University of Thessaloniki, Thessaloniki"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ioannis 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