{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,24]],"date-time":"2026-03-24T19:19:46Z","timestamp":1774379986586,"version":"3.50.1"},"reference-count":43,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"6","content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2022,8]]},"abstract":"<jats:sec>\n            <jats:title>Background<\/jats:title>\n            <jats:p>Significant changes in CpG methylation have been identified in nasal polyps, which are the main targets of nonsteroidal anti-inflammatory drug-exacerbated respiratory disease (NERD); however, these polyps are composed of various cellular components. In the present study, whole-genome CpG methylation in peripheral blood lymphocytes (PBLs) was analyzed to define the epigenetic changes in lymphocytes, which are the primary immune cells involved in NERD.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Materials and methods<\/jats:title>\n            <jats:p>Genomic DNA from peripheral blood mononuclear cells from 27 NERD and 24 aspirin-tolerant asthma (ATA) was subjected to bisulfate conversion and a methylation array. Quantitative CpG methylation, the \u03b2-values as a quantitative measure of DNA methylation, in lymphocytes were calculated after adjustments for cellular composition.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>Fifty-six hypermethylated and three hypomethylated differentially methylated CpGs (DMCs) in PBLs in the NERD compared with ATA. The top 10 CpG loci predicted the methylation risk score, with a positive predictive value of 91.3%, a negative predictive value of 81.5% and an accuracy of 84.3%. As demonstrated in the nasal polyps, 30 DMCs were predicted to bind to the following 10 transcription factors, ranked in descending order: AP-2alphaA, TFII-1, STAT4, FOXP3, GR, c-Est-1, E2F-1, XBP1, ENKTF-1 and NF-1. Gene ontology analysis identified 13 categories such as regulation of T-helper 17 cell differentiation, including SMAD7 and NFKBIZ. PBLs in NERD contained no DMCs in genes associated with the prostaglandin and leukotriene pathways, which were found in ATA.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusion<\/jats:title>\n            <jats:p>PBLs in NERD form a unique pattern of DNA CpG methylation, and the combined analysis may provide predictive values for NERD.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1097\/fpc.0000000000000475","type":"journal-article","created":{"date-parts":[[2022,6,13]],"date-time":"2022-06-13T12:01:11Z","timestamp":1655121671000},"page":"226-234","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":5,"title":["Genome-wide DNA methylation profile of peripheral blood lymphocytes from subjects with nonsteroidal anti-inflammatory drug-induced respiratory diseases"],"prefix":"10.1097","volume":"32","author":[{"given":"Jong-Uk","family":"Lee","sequence":"first","affiliation":[{"name":"Division of Allergy and Respiratory Medicine, Department of Internal Medicine, Soonchunhyang University Bucheon Hospital"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hun","family":"Soo Chang","sequence":"additional","affiliation":[{"name":"Department of Anatomy and BK21 FOUR Project, College of Medicine, Soonchunhyang University, Cheonan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Min","family":"Kyung Kim","sequence":"additional","affiliation":[{"name":"Department of Interdisciplinary Program in Biomedical Science Major, Soonchuhyang University"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seung-lee","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Interdisciplinary Program in Biomedical Science Major, Soonchuhyang University"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jung Hyun","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Korean Armed Forces Capital Hospital, Seongnam"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jong-Sook","family":"Park","sequence":"additional","affiliation":[{"name":"Division of Allergy and Respiratory Medicine, Department of Internal Medicine, Soonchunhyang University Bucheon Hospital"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Choon-Sik","family":"Park","sequence":"additional","affiliation":[{"name":"Division of Allergy and Respiratory Medicine, Department of Internal Medicine, Soonchunhyang University Bucheon Hospital"},{"name":"PulmoBioPark Co., Ltd., Soonchunhyang University Bucheon Hospital, Bucheon, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"276","published-online":{"date-parts":[[2022,6,9]]},"reference":[{"key":"R1-20250808","doi-asserted-by":"crossref","first-page":"1111","DOI":"10.1111\/j.1398-9995.2007.01409.x","article-title":"EAACI\/GA2LEN guideline: aspirin provocation tests for diagnosis of aspirin hypersensitivity.","volume":"62","author":"Nizankowska-Mogilnicka","year":"2007","journal-title":"Allergy"},{"key":"R2-20250808","doi-asserted-by":"crossref","first-page":"256","DOI":"10.4168\/aair.2011.3.4.256","article-title":"Diagnostic value of clinical parameters in the prediction of aspirin-exacerbated respiratory disease in asthma.","volume":"3","author":"Chang","year":"2011","journal-title":"Allergy Asthma Immunol Res"},{"key":"R3-20250808","doi-asserted-by":"crossref","first-page":"432","DOI":"10.1034\/j.1399-3003.2000.016003432.x","article-title":"Natural history of aspirin-induced asthma. 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