{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T01:12:56Z","timestamp":1779325976315,"version":"3.51.4"},"reference-count":7,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,10]]},"abstract":"<jats:sec>\n            <jats:title>Background:<\/jats:title>\n            <jats:p>Aminoglycosides require highly accurate therapeutic drug monitoring owing to their narrow therapeutic windows and toxic side effects. Therapeutic drug monitoring varies in different laboratories, and this difference is mainly due to the use of different analytical techniques. This study aimed to compare the accuracy and precision of immunoassays for the measurement of gentamicin, tobramycin, and amikacin in serum.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Methods:<\/jats:title>\n            <jats:p>Human plasma samples were spiked with known concentrations of amikacin, gentamicin, and tobramycin and dispatched to laboratories worldwide. The percentage deviation and coefficient of variation were calculated to compare the accuracy and precision among immunoassays and among antibiotics.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results:<\/jats:title>\n            <jats:p>We analyzed 273, 534, and 207 amikacin, gentamicin, and tobramycin measurement results, obtained satisfactory rates of 83.9%, 86.3%, and 93.7%, and coefficients of variation ranging from 1.1% to 15.6%, 2.9% to 25.2%, and 1.8% to 27.0%, respectively. The percentage deviation ranged from \u22127.5% to 6.6%, \u221220.8% to 18.7%, and \u221233.2% to 41.5% for amikacin, gentamicin, and tobramycin, respectively. Significant differences were observed in accuracy and precision among assays for all antibiotics.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusions:<\/jats:title>\n            <jats:p>This study demonstrated high variations in results obtained from antibiotic assays conducted at different laboratories worldwide.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1097\/ftd.0000000000000752","type":"journal-article","created":{"date-parts":[[2020,3,2]],"date-time":"2020-03-02T03:15:42Z","timestamp":1583118942000},"page":"710-715","source":"Crossref","is-referenced-by-count":4,"title":["Evaluation of Assays to Measure Aminoglycosides in Serum: Comparison of Accuracy and Precision Based on External Quality Assessment"],"prefix":"10.1097","volume":"42","author":[{"given":"Mengya","family":"Li","sequence":"first","affiliation":[{"name":"Department of Pharmacy, Peking University First Hospital; and"},{"name":"Department of Pharmacy Administration and Clinical Pharmacy, School of Pharmaceutical Sciences, Peking University Health Science Center, Beijing, People's Republic of China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lingyun","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Peking University First Hospital; and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chaoyang","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Peking University First Hospital; and"},{"name":"Department of Pharmacy Administration and Clinical Pharmacy, School of Pharmaceutical Sciences, Peking University Health Science Center, Beijing, People's Republic of China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lili","family":"Lei","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Peking University First Hospital; and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xingyu","family":"Zhai","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Peking University First Hospital; and"},{"name":"Department of Pharmacy Administration and Clinical Pharmacy, School of Pharmaceutical Sciences, Peking University Health Science Center, Beijing, People's Republic of China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daohuang","family":"Luo","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Peking University First Hospital; and"},{"name":"Department of Pharmacy Administration and Clinical Pharmacy, School of Pharmaceutical Sciences, Peking University Health Science Center, Beijing, People's Republic of China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ying","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Peking University First Hospital; and"},{"name":"Department of Pharmacy Administration and Clinical Pharmacy, School of Pharmaceutical Sciences, Peking University Health Science Center, Beijing, People's Republic of China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yimin","family":"Cui","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Peking University First Hospital; and"},{"name":"Department of Pharmacy Administration and Clinical Pharmacy, School of Pharmaceutical Sciences, Peking University Health Science Center, Beijing, People's Republic of China."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"276","reference":[{"key":"R1-20230928","doi-asserted-by":"crossref","first-page":"8","DOI":"10.4314\/gmj.v53i1.2","article-title":"Standards of aminoglycoside therapeutic drug monitoring in a South African private hospital: perspectives and implications","volume":"53","author":"du Toit","year":"2019","journal-title":"Ghana Med J."},{"key":"R2-20230928","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1097\/00007691-199902000-00010","article-title":"Impact of goal-oriented and model-based clinical pharmacokinetic dosing of aminoglycosides on clinical outcome: a cost-effectiveness analysis","volume":"21","author":"van Lent-Evers","year":"1999","journal-title":"Ther Drug Monit."},{"key":"R3-20230928","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1093\/jac\/dkg296","article-title":"Evaluation of commercial assays for vancomycin and aminoglycosides in serum: a comparison of accuracy and precision based on external quality assessment","volume":"52","author":"Wilson","year":"2003","journal-title":"J Antimicrob Chemother."},{"key":"R4-20230928","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1016\/j.clinbiochem.2019.02.001","article-title":"Insufficient harmonization of antibiotics assays - polish experience with an external quality assessment program in the years 2011-2018","volume":"66","author":"Kowalski","year":"2019","journal-title":"Clin Biochem."},{"key":"R5-20230928","doi-asserted-by":"crossref","first-page":"686","DOI":"10.1097\/01.ftd.0000243966.97964.11","article-title":"Pitfalls in TDM of antibiotic drugs: analytical and modelling issues","volume":"28","author":"Neef","year":"2006","journal-title":"Ther Drug Monit."},{"key":"R6-20230928","doi-asserted-by":"crossref","first-page":"1331","DOI":"10.1248\/bpb.b16-00249","article-title":"Inter-laboratory variability of current immunoassay methods for tacrolimus among Japanese hospitals","volume":"39","author":"Miura","year":"2016","journal-title":"Biol Pharm Bull."},{"key":"R7-20230928","doi-asserted-by":"crossref","first-page":"657","DOI":"10.1097\/FTD.0000000000000632","article-title":"Inter-laboratory analysis of Isavuconazole plasma concentration assays among European laboratories","volume":"41","author":"Pea","year":"2019","journal-title":"Ther Drug Monit."}],"container-title":["Therapeutic Drug Monitoring"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.lww.com\/10.1097\/FTD.0000000000000752","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,28]],"date-time":"2023-09-28T01:05:52Z","timestamp":1695863152000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.lww.com\/10.1097\/FTD.0000000000000752"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,10]]},"references-count":7,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2020]]}},"URL":"https:\/\/doi.org\/10.1097\/ftd.0000000000000752","relation":{},"ISSN":["0163-4356"],"issn-type":[{"value":"0163-4356","type":"print"}],"subject":[],"published":{"date-parts":[[2020,10]]}}}