{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T10:14:39Z","timestamp":1778580879969,"version":"3.51.4"},"reference-count":29,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"3","content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:sec>\n                    <jats:title>Background:<\/jats:title>\n                    <jats:p>The endocannabinoid system regulates immune function, inflammation, and metabolism and has gained increasing attention in clinical research. However, the instability, low abundance, and physicochemical complexity of endocannabinoids (ECs) and EC-like substances (EC-like) make their quantification in plasma analytically challenging.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective and methods:<\/jats:title>\n                    <jats:p>The authors developed and validated a liquid chromatography\u2013tandem mass spectrometry method for the simultaneous quantification of N-arachidonoylethanolamide (AEA), 2-arachidonoylglycerol, and its isomer 1-arachidonoylglycerol (2-AG\/1-AG), oleoylethanolamide (OEA), and palmitoylethanolamide (PEA) in human plasma.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>\n                      Extraction was performed using liquid\u2013liquid extraction combined with liquid chromatography\u2013tandem mass spectrometry. The method achieved lower limits of quantification of 0.1 mcg\/L for AEA, 0.2 mcg\/L for 2-AG\/1-AG, and 0.5 mcg\/L for OEA and PEA, with calibration curves showing high linearity (R\n                      <jats:sup>2<\/jats:sup>\n                      \u2265 0.995). Intra-assay and interassay accuracy and precision were both within 15%. Additional validation parameters, including selectivity, recovery, carryover, matrix suitability, and dilution integrity, fulfilled regulatory criteria. Preanalytical handling significantly influenced concentrations: Delayed centrifugation and postthaw storage increased AEA, OEA, and PEA levels. The validated method was applied to plasma samples from patients coinfected with HIV and hepatitis C virus, enabling reproducible quantification of ECs and EC-like substances in a clinically relevant cohort.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions:<\/jats:title>\n                    <jats:p>The study demonstrates that biological and technical factors markedly affect plasma EC and EC-like concentrations. Standardized preanalytical processing is therefore essential for accurate measurement, and the proposed method provides a robust tool for clinical and pharmacological research.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1097\/ftd.0000000000001397","type":"journal-article","created":{"date-parts":[[2025,10,2]],"date-time":"2025-10-02T04:01:29Z","timestamp":1759377689000},"page":"379-386","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":0,"title":["Liquid Chromatography\u2013Tandem Mass Spectrometry Method for Simultaneous Quantification of Four Endocannabinoids and Endocannabinoid-Like Substances in Plasma: Application in an HIV\u2014Hepatitis C Virus Coinfected Population"],"prefix":"10.1097","volume":"48","author":[{"given":"Alexandr","family":"Gish","sequence":"first","affiliation":[{"name":"CHU Lille, Unit\u00e9 Fonctionnelle de Toxicologie, Lille, France;"},{"name":"Univ. Lille, ULR 4483\u2014IMPECS\u2014IMPact de l'Environnement Chimique sur la Sant\u00e9 humaine, Lille, France; and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eqbal","family":"Radwan","sequence":"additional","affiliation":[{"name":"Aix Marseille Univ, INSERM, IRD, SESSTIM, Sciences \u00c9conomiques & Sociales de la Sant\u00e9 & Traitement de l'Information M\u00e9dicale, ISSPAM, Marseille, France."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Camille","family":"Richeval","sequence":"additional","affiliation":[{"name":"CHU Lille, Unit\u00e9 Fonctionnelle de Toxicologie, Lille, France;"},{"name":"Univ. Lille, ULR 4483\u2014IMPECS\u2014IMPact de l'Environnement Chimique sur la Sant\u00e9 humaine, Lille, France; and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Camelia","family":"Protopopescu","sequence":"additional","affiliation":[{"name":"Aix Marseille Univ, INSERM, IRD, SESSTIM, Sciences \u00c9conomiques & Sociales de la Sant\u00e9 & Traitement de l'Information M\u00e9dicale, ISSPAM, Marseille, France."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jean-Fran\u00e7ois","family":"Wiart","sequence":"additional","affiliation":[{"name":"CHU Lille, Unit\u00e9 Fonctionnelle de Toxicologie, Lille, France;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Florian","family":"Hakim","sequence":"additional","affiliation":[{"name":"CHU Lille, Unit\u00e9 Fonctionnelle de Toxicologie, Lille, France;"},{"name":"Univ. Lille, ULR 4483\u2014IMPECS\u2014IMPact de l'Environnement Chimique sur la Sant\u00e9 humaine, Lille, France; and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Delphine","family":"Allorge","sequence":"additional","affiliation":[{"name":"CHU Lille, Unit\u00e9 Fonctionnelle de Toxicologie, Lille, France;"},{"name":"Univ. Lille, ULR 4483\u2014IMPECS\u2014IMPact de l'Environnement Chimique sur la Sant\u00e9 humaine, Lille, France; and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patrizia","family":"Carrieri","sequence":"additional","affiliation":[{"name":"Aix Marseille Univ, INSERM, IRD, SESSTIM, Sciences \u00c9conomiques & Sociales de la Sant\u00e9 & Traitement de l'Information M\u00e9dicale, ISSPAM, Marseille, France."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tangui","family":"Barr\u00e9","sequence":"additional","affiliation":[{"name":"Aix Marseille Univ, INSERM, IRD, SESSTIM, Sciences \u00c9conomiques & Sociales de la Sant\u00e9 & Traitement de l'Information M\u00e9dicale, ISSPAM, Marseille, France."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1111-4671","authenticated-orcid":false,"given":"Jean-Michel","family":"Gaulier","sequence":"additional","affiliation":[{"name":"CHU Lille, Unit\u00e9 Fonctionnelle de Toxicologie, Lille, France;"},{"name":"Univ. 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