{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T22:42:44Z","timestamp":1785451364468,"version":"3.56.0"},"reference-count":39,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"2","license":[{"start":{"date-parts":[[2023,1,20]],"date-time":"2023-01-20T00:00:00Z","timestamp":1674172800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/ http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"abstract":"<jats:sec>\n            <jats:title>Background and Aims:<\/jats:title>\n            <jats:p>Restitution of the extrahepatic biliary luminal epithelium in cholangiopathies is poorly understood. Prominin-1 (Prom1) is a key component of epithelial ciliary body of stem\/progenitor cells. Given that intrahepatic Prom1-expressing progenitor cells undergo cholangiocyte differentiation, we hypothesized that Prom1 may promote restitution of the extrahepatic bile duct (EHBD) epithelium following injury.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Approach and Results:<\/jats:title>\n            <jats:p>Utilizing various murine biliary injury models, we identified <jats:italic toggle=\"yes\">Prom1<\/jats:italic>-expressing cells in the peribiliary glands of the EHBD. These <jats:italic toggle=\"yes\">Prom1<\/jats:italic>-expressing cells are progenitor cells which give rise to cholangiocytes as part of the normal maintenance of the EHBD epithelium. Following injury, these cells proliferate significantly more rapidly to re-populate the biliary luminal epithelium. Null mutation of <jats:italic toggle=\"yes\">Prom1<\/jats:italic> leads to significantly &gt;10-fold dilated peribiliary glands following rhesus rotavirus\u2013mediated biliary injury. Cultured organoids derived from <jats:italic toggle=\"yes\">Prom1<\/jats:italic> knockout mice are comprised of biliary progenitor cells with altered apical-basal cellular polarity, significantly fewer and shorter cilia, and decreased organoid proliferation dynamics consistent with impaired cell motility.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusions:<\/jats:title>\n            <jats:p>We, therefore, conclude that Prom1 is involved in biliary epithelial restitution following biliary injury in part through its role in supporting cell polarity.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1097\/hc9.0000000000000018","type":"journal-article","created":{"date-parts":[[2023,1,20]],"date-time":"2023-01-20T19:00:53Z","timestamp":1674241253000},"page":"e0018-e0018","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":7,"title":["Prominin-1 promotes restitution of the murine extrahepatic biliary luminal epithelium following cholestatic liver injury"],"prefix":"10.1097","volume":"7","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8102-6696","authenticated-orcid":false,"given":"Allen","family":"Zhong","sequence":"first","affiliation":[{"name":"Developmental Biology, Regenerative Medicine, and Stem Cell Program, The Saban Research Institute, Children\u2019s Hospital of Los Angeles, Los Angeles, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4457-0123","authenticated-orcid":false,"given":"Celia","family":"Short","sequence":"additional","affiliation":[{"name":"Developmental Biology, Regenerative Medicine, and Stem Cell Program, The Saban Research Institute, Children\u2019s Hospital of Los Angeles, Los Angeles, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2314-8089","authenticated-orcid":false,"given":"Jiabo","family":"Xu","sequence":"additional","affiliation":[{"name":"Developmental Biology, Regenerative Medicine, and Stem Cell Program, The Saban Research Institute, Children\u2019s Hospital of Los Angeles, Los Angeles, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8393-9229","authenticated-orcid":false,"given":"G. Esteban","family":"Fernandez","sequence":"additional","affiliation":[{"name":"Cellular Imaging Core, The Saban Research Institute, Children\u2019s Hospital of Los Angeles, Los Angeles, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3519-6790","authenticated-orcid":false,"given":"Nicolas","family":"Malkoff","sequence":"additional","affiliation":[{"name":"Developmental Biology, Regenerative Medicine, and Stem Cell Program, The Saban Research Institute, Children\u2019s Hospital of Los Angeles, Los Angeles, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6573-1611","authenticated-orcid":false,"given":"Nicolas","family":"Noriega","sequence":"additional","affiliation":[{"name":"Developmental Biology, Regenerative Medicine, and Stem Cell Program, The Saban Research Institute, Children\u2019s Hospital of Los Angeles, Los Angeles, California, 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