{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T09:06:35Z","timestamp":1784106395520,"version":"3.55.0"},"reference-count":14,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2019,6]]},"abstract":"<jats:sec>\n            <jats:title>Purpose:<\/jats:title>\n            <jats:p>To compare the variation in corneal endothelial cell density (ECD) from the center to the periphery in unpeeled and peeled donor corneas and to determine the impact of eccentric trephining on total endothelial cells in Descemet membrane endothelial keratoplasty (DMEK) grafts.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Methods:<\/jats:title>\n            <jats:p>Mated donor cornea pairs were obtained. One cornea from each pair was peeled for DMEK, whereas the other was left unpeeled. Alizarin Red was used to stain the endothelial cells. High-resolution images at fixed magnification were obtained for the center, midperiphery (2.5 mm from the center), and the periphery (5 mm from the center). The cells were then counted, and ECD was calculated by a masked evaluator using ImageJ software. Regression analysis was then performed to evaluate the change in ECD as a function of radius (distance from the corneal center). The impact of eccentric trephining on total endothelial cells in a given DMEK graft was then calculated using numerical integration.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results:<\/jats:title>\n            <jats:p>Ten pairs of corneas were evaluated. ECD increased by 1.4% (40.0 cells\/mm<jats:sup>2<\/jats:sup>) (<jats:italic toggle=\"yes\">P<\/jats:italic> = 0.03) for peeled corneas and 1.8% (51.5 cells\/mm<jats:sup>2<\/jats:sup>) (<jats:italic toggle=\"yes\">P<\/jats:italic> &lt; 0.01) for unpeeled corneas for each millimeter from the center. There was no difference between peeled and unpeeled corneas in the mean central (<jats:italic toggle=\"yes\">P<\/jats:italic> = 0.98) or peripheral (<jats:italic toggle=\"yes\">P<\/jats:italic> = 0.35) ECD. Based on the increase in ECD as a function of radius, eccentric trephining of a 7.5-mm DMEK graft by 2.25 mm yields 0.95% more total endothelial cells per graft.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusions:<\/jats:title>\n            <jats:p>Corneal ECD increases from the center to the periphery in both peeled and unpeeled corneas. Eccentric trephining increases the number of transplanted endothelial cells per graft.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1097\/ico.0000000000001925","type":"journal-article","created":{"date-parts":[[2019,3,20]],"date-time":"2019-03-20T19:25:10Z","timestamp":1553109910000},"page":"748-753","source":"Crossref","is-referenced-by-count":6,"title":["Peripheral Endothelial Cell Density in Descemet Membrane Endothelial Keratoplasty Grafts"],"prefix":"10.1097","volume":"38","author":[{"given":"Brian A.","family":"Lee","sequence":"first","affiliation":[{"name":"Department of Ophthalmology and Visual Neurosciences, University of Minnesota, Minneapolis, MN;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saima","family":"Qureshi","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology and Visual Neurosciences, University of Minnesota, Minneapolis, MN;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sung","family":"Lee","sequence":"additional","affiliation":[{"name":"Lions Gift of Sight Eye Bank, St. Paul, MN; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gene J.","family":"Hou","sequence":"additional","affiliation":[{"name":"Department of Mechanical and Aerospace Engineering, Old Dominion University, Norfolk, VA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Bedard","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology and Visual Neurosciences, University of Minnesota, Minneapolis, MN;"},{"name":"Lions Gift of Sight Eye Bank, St. Paul, MN; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joshua H.","family":"Hou","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology and Visual Neurosciences, University of Minnesota, Minneapolis, MN;"},{"name":"Lions Gift of Sight Eye Bank, St. Paul, MN; and"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","published-online":{"date-parts":[[2019,4,11]]},"reference":[{"key":"R1-20230912","doi-asserted-by":"crossref","first-page":"774","DOI":"10.1016\/S0002-9394(03)00807-9","article-title":"Increased endothelial cell density in the paracentral and peripheral regions of the human cornea","volume":"136","author":"Raj","year":"2003","journal-title":"Am J Ophthalmol."},{"key":"R2-20230912","first-page":"223","article-title":"Direct and indirect determination of nonuniform cell density distribution in human corneal endothelium","volume":"25","author":"Schimmelpfennig","year":"1984","journal-title":"Invest Ophthalmol Vis Sci."},{"key":"R3-20230912","first-page":"816","article-title":"Evidence suggesting the existence of stem cells for the human corneal endothelium","volume":"11","author":"Whikehart","year":"2005","journal-title":"Mol 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(Copenh)"},{"key":"R7-20230912","doi-asserted-by":"crossref","first-page":"1425","DOI":"10.1136\/bjophthalmol-2017-311398","article-title":"Quarter-Descemet membrane endothelial keratoplasty (Quarter-DMEK) for Fuchs endothelial corneal dystrophy: 6 months clinical outcome","volume":"102","author":"Zygoura","year":"2018","journal-title":"Br J Ophthalmol."},{"key":"R8-20230912","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1097\/ICO.0000000000001368","article-title":"Donor tissue preparation for Descemet membrane endothelial keratoplasty: an updated review","volume":"37","author":"Birbal","year":"2018","journal-title":"Cornea"},{"key":"R9-20230912","doi-asserted-by":"crossref","first-page":"1382","DOI":"10.1097\/ICO.0b013e31821ddd25","article-title":"Endothelial keratoplasty: fellow eyes comparison of Descemet stripping automated endothelial keratoplasty and Descemet membrane endothelial keratoplasty","volume":"30","author":"Guerra","year":"2011","journal-title":"Cornea"},{"key":"R10-20230912","doi-asserted-by":"crossref","first-page":"2361","DOI":"10.1016\/j.ophtha.2009.07.010","article-title":"Descemet's membrane endothelial keratoplasty: prospective multicenter study of visual and refractive outcomes and endothelial survival","volume":"116","author":"Price","year":"2009","journal-title":"Ophthalmology"},{"key":"R11-20230912","doi-asserted-by":"crossref","first-page":"818","DOI":"10.1097\/ICO.0b013e3181705ca2","article-title":"An easy and inexpensive method for quantitative analysis of endothelial damage by using vital dye staining and Adobe Photoshop software","volume":"27","author":"Saad","year":"2008","journal-title":"Cornea"},{"key":"R12-20230912","doi-asserted-by":"crossref","first-page":"1564","DOI":"10.1136\/bjophthalmol-2015-307783","article-title":"Preliminary outcome of hemi-Descemet membrane endothelial keratoplasty for Fuchs endothelial dystrophy","volume":"100","author":"Gerber-Hollbach","year":"2016","journal-title":"Br J Ophthalmol."},{"key":"R13-20230912","doi-asserted-by":"crossref","first-page":"420","DOI":"10.1136\/bjophthalmol-2015-307335","article-title":"Graft preparation for hemi-Descemet membrane endothelial keratoplasty (hemi-DMEK)","volume":"100","author":"Lie","year":"2016","journal-title":"Br J Ophthalmol"},{"key":"R14-20230912","doi-asserted-by":"crossref","first-page":"e129","DOI":"10.1111\/aos.12520","article-title":"Bubble technique for Descemet membrane endothelial keratoplasty tissue preparation in an eye bank: air or liquid?","volume":"93","author":"Ruzza","year":"2015","journal-title":"Acta Ophthalmol (Copenh)"}],"container-title":["Cornea"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.lww.com\/00003226-201906000-00014","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,12]],"date-time":"2023-09-12T19:34:46Z","timestamp":1694547286000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.lww.com\/00003226-201906000-00014"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,4,11]]},"references-count":14,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2019]]}},"URL":"https:\/\/doi.org\/10.1097\/ico.0000000000001925","relation":{},"ISSN":["0277-3740"],"issn-type":[{"value":"0277-3740","type":"print"}],"subject":[],"published":{"date-parts":[[2019,4,11]]}}}