{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,23]],"date-time":"2025-12-23T15:41:59Z","timestamp":1766504519737,"version":"3.41.0"},"reference-count":37,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"1","content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2021,1]]},"abstract":"<jats:sec>\n            <jats:title>Background:<\/jats:title>\n            <jats:p>Recurrent acute otitis media (RAOM) is common in children, and it may result in spontaneous tympanic membrane perforation (STMP), management of which is often challenging. In the upper respiratory tract (URT), resident microorganisms play a pivotal role in otitis media pathogenesis and prevention, as they are able to inhibit the colonization process and otopathogens growth. In particular, <jats:italic toggle=\"yes\">Dolosigranulum spp.<\/jats:italic> and <jats:italic toggle=\"yes\">Corynebacterium spp<\/jats:italic>. have been associated with respiratory health in several studies. This study aims at comparing both nasopharyngeal microbiota of children with RAOM versus matched controls and nasopharyngeal microbiota of children with a history of RAOM with STMP.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Method:<\/jats:title>\n            <jats:p>Nasopharyngeal swabs were collected from 132 children, median age 3.51 (2.13\u20134.72), including 36 healthy children, 50 with RAOM without STMP, and 46 with RAOM with STMP. Bacterial DNA was subsequently extracted and 16S rRNA gene V3-V4 regions were polymerase chain reaction amplified and sequenced using Illumina MiSeq technology.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results:<\/jats:title>\n            <jats:p>A higher relative abundance of <jats:italic toggle=\"yes\">Dolosigranulum<\/jats:italic> and <jats:italic toggle=\"yes\">Corynebacterium<\/jats:italic> genera was detected in the nasopharynx of healthy children (16.5% and 9.3%, respectively) in comparison with RAOM without STMP (8.9% and 4.3%, respectively) and RAOM with STMP (5.2% and 2.8%, respectively). A decreasing pattern in relative abundance of these 2 pivotal genera through disease severity was detected. In all groups, the most abundant genera were <jats:italic toggle=\"yes\">Moraxella<\/jats:italic>, <jats:italic toggle=\"yes\">Streptococcus<\/jats:italic> and <jats:italic toggle=\"yes\">Haemophilus<\/jats:italic>, followed by <jats:italic toggle=\"yes\">Dolosigranulum<\/jats:italic> and <jats:italic toggle=\"yes\">Corynebacterium.<\/jats:italic>\n                              <\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusions:<\/jats:title>\n            <jats:p>Our study provides a characterization of the URT microbiota in otitis-prone children with and without history of recurrent STMP, suggesting that the role of <jats:italic toggle=\"yes\">Dolosigranulum<\/jats:italic> and <jats:italic toggle=\"yes\">Corynebacterium<\/jats:italic> in regulating the healthy URT microbiota should be further studied.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1097\/inf.0000000000002895","type":"journal-article","created":{"date-parts":[[2020,10,14]],"date-time":"2020-10-14T19:51:03Z","timestamp":1602705063000},"page":"16-21","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":12,"title":["Nasopharyngeal Microbiota Analysis in Healthy and Otitis-prone Children"],"prefix":"10.1097","volume":"40","author":[{"given":"Francesco","family":"Folino","sequence":"first","affiliation":[{"name":"Department of Pathophysiology and Transplantation, University of Milan, Milan, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Miriam","family":"Fattizzo","sequence":"additional","affiliation":[{"name":"Fondazione IRCCS Ca\u2019 Granda Ospedale Maggiore Policlinico, Milan, Italy"},{"name":"Department of Pediatrics, ASST Sette Laghi, Del Ponte Hospital, Varese, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luca","family":"Ruggiero","sequence":"additional","affiliation":[{"name":"Department of Pathophysiology and Transplantation, University of Milan, Milan, Italy"},{"name":"Fondazione IRCCS Ca\u2019 Granda Ospedale Maggiore Policlinico, Milan, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martina","family":"Oriano","sequence":"additional","affiliation":[{"name":"Department of Pathophysiology and Transplantation, University of Milan, Milan, Italy"},{"name":"Fondazione IRCCS Ca\u2019 Granda Ospedale Maggiore Policlinico, Milan, Italy"},{"name":"Department of Molecular Medicine, University of Pavia, Pavia, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefano","family":"Aliberti","sequence":"additional","affiliation":[{"name":"Department of Pathophysiology and Transplantation, University of Milan, Milan, Italy"},{"name":"Fondazione IRCCS Ca\u2019 Granda Ospedale Maggiore Policlinico, Milan, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francesco","family":"Blasi","sequence":"additional","affiliation":[{"name":"Department of Pathophysiology and Transplantation, University of Milan, Milan, Italy"},{"name":"Fondazione IRCCS Ca\u2019 Granda Ospedale Maggiore Policlinico, Milan, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michele","family":"Gaffuri","sequence":"additional","affiliation":[{"name":"Fondazione IRCCS Ca\u2019 Granda Ospedale Maggiore Policlinico, Milan, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paola","family":"Marchisio","sequence":"additional","affiliation":[{"name":"Department of Pathophysiology and Transplantation, University of Milan, Milan, Italy"},{"name":"Fondazione IRCCS Ca\u2019 Granda Ospedale Maggiore Policlinico, Milan, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sara","family":"Torretta","sequence":"additional","affiliation":[{"name":"Fondazione IRCCS Ca\u2019 Granda Ospedale Maggiore Policlinico, Milan, Italy"},{"name":"Department of Clinical Sciences and Community Health, University of Milan, Milan, Italy."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"276","published-online":{"date-parts":[[2020,10,12]]},"reference":[{"key":"R1-20250615","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1093\/infdis\/160.1.83","article-title":"Epidemiology of otitis media during the first seven years of life in children in greater Boston: a prospective, cohort study.","volume":"160","author":"Teele","year":"1989","journal-title":"J Infect Dis"},{"key":"R2-20250615","doi-asserted-by":"crossref","first-page":"e0150949","DOI":"10.1371\/journal.pone.0150949","article-title":"Predominant bacteria detected from the middle ear fluid of children experiencing otitis media: a systematic review.","volume":"11","author":"Ngo","year":"2016","journal-title":"PLoS One"},{"key":"R3-20250615","doi-asserted-by":"crossref","first-page":"1440","DOI":"10.1086\/516477","article-title":"Relationship between nasopharyngeal colonization and the development of otitis media in children. 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