{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,15]],"date-time":"2026-03-15T12:35:07Z","timestamp":1773578107055,"version":"3.50.1"},"reference-count":13,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"27","license":[{"start":{"date-parts":[[2022,7,8]],"date-time":"2022-07-08T00:00:00Z","timestamp":1657238400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"abstract":"<jats:sec>\n            <jats:title>Introduction:<\/jats:title>\n            <jats:p>\n                                 <jats:italic toggle=\"yes\">Streptococcus agalactiae<\/jats:italic> is a common pathogen in infective endocarditis, but the positive rate of traditional blood culture diagnosis is not high. It is challenging to obtain a good outcome in the absence of pathogen information for patients with infectious endocarditis.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Patient concerns and diagnosis:<\/jats:title>\n            <jats:p>Here, we report the case of a patient with infective endocarditis caused by <jats:italic toggle=\"yes\">S. agalactiae<\/jats:italic>. The initial manifestations of this patient were coma, urinary incontinence, and fecal incontinence and had no history of heart disease or infectious diseases before admission.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Interventions and outcomes:<\/jats:title>\n            <jats:p>When the blood culture was negative 3 consecutive times, the pathogen <jats:italic toggle=\"yes\">S. agalactiae<\/jats:italic> was diagnosed in a timely and accurate manner by metagenome sequencing. Eventually, the patient was discharged following surgery and antibiotic treatment.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusions:<\/jats:title>\n            <jats:p>For IE patients with infective endocarditis, metagenome sequencing is a valuable and selective tool for rapid, sensitive, and accurate pathogen detection, especially when the blood culture is negative.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1097\/md.0000000000029360","type":"journal-article","created":{"date-parts":[[2022,7,8]],"date-time":"2022-07-08T12:08:35Z","timestamp":1657282115000},"page":"e29360","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":2,"title":["Infectious native valve endocarditis by Streptococcus agalactiae species: Case report of pathogen identification only through metagenomic sequencing technology"],"prefix":"10.1097","volume":"101","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9206-5359","authenticated-orcid":false,"given":"Ruoxin","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Cardiovascular Surgery, Shanghai Jiaotong University Affiliated Sixth People\u2019s Hospital, Shanghai, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xuejie","family":"Cao","sequence":"additional","affiliation":[{"name":"Genoxor Medical Science and Technology Inc., Shanghai, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fang","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Intensive Care Unit, Shanghai Jiaotong University Affiliated Sixth People\u2019s Hospital, Shanghai, China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jinlong","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Cardiovascular Surgery, Shanghai Jiaotong University Affiliated Sixth People\u2019s Hospital, Shanghai, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liang","family":"Fu","sequence":"additional","affiliation":[{"name":"Department of Cardiovascular Surgery, Shanghai Jiaotong University Affiliated Sixth People\u2019s Hospital, Shanghai, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ziming","family":"Yuan","sequence":"additional","affiliation":[{"name":"Department of Intensive Care Unit, Shanghai Jiaotong University Affiliated Sixth People\u2019s Hospital, Shanghai, China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yinkai","family":"Ni","sequence":"additional","affiliation":[{"name":"Department of Cardiovascular Surgery, Shanghai Jiaotong University Affiliated Sixth People\u2019s Hospital, Shanghai, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zonghui","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Cardiovascular Surgery, Shanghai Jiaotong University Affiliated Sixth People\u2019s Hospital, Shanghai, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Feng","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Cardiovascular Surgery, Shanghai Jiaotong University Affiliated Sixth People\u2019s Hospital, Shanghai, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"276","published-online":{"date-parts":[[2022,7,8]]},"reference":[{"key":"R1-20250504","doi-asserted-by":"crossref","first-page":"104416","DOI":"10.1016\/j.micpath.2020.104416","article-title":"Genome reanalysis to decipher resistome, virulome, and attenuated characters of attenuated Streptococcus agalactiae strain HZAUSC001.","volume":"147","author":"Zhang","year":"2020","journal-title":"Microb Pathog"},{"key":"R2-20250504","doi-asserted-by":"crossref","first-page":"2333","DOI":"10.2169\/internalmedicine.54.4709","article-title":"Two case reports of group B streptococcal infective endocarditis complicated by embolism.","volume":"54","author":"Aoyama","year":"2015","journal-title":"Intern Med"},{"key":"R3-20250504","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1016\/j.jinf.2010.04.005","article-title":"Streptococcus agalactiae left-sided infective endocarditis. Analysis of 27 cases from a multicentric cohort.","volume":"61","author":"Ivanova Georgieva","year":"2010","journal-title":"J Infect"},{"key":"R4-20250504","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1086\/599369","article-title":"Increasing burden of invasive group B streptococcal disease in nonpregnant adults, 1990-2007.","volume":"49","author":"Skoff","year":"2009","journal-title":"Clin Infect Dis"},{"key":"R5-20250504","doi-asserted-by":"crossref","first-page":"882","DOI":"10.1016\/S0140-6736(15)00067-7","article-title":"Infective endocarditis.","volume":"387","author":"Cahill","year":"2016","journal-title":"Lancet"},{"key":"R6-20250504","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.medmal.2014.11.003","article-title":"Update on blood culture-negative endocarditis.","volume":"45","author":"Tattevin","year":"2015","journal-title":"Med Mal Infect"},{"key":"R7-20250504","doi-asserted-by":"crossref","first-page":"1558","DOI":"10.1253\/circj.CJ-12-1101","article-title":"Recent picture of infective endocarditis in Japan.","volume":"77","author":"Satoshi","year":"2013","journal-title":"Circ J"},{"key":"R8-20250504","doi-asserted-by":"crossref","first-page":"965","DOI":"10.1016\/S0140-6736(11)60755-1","article-title":"Management of infective endocarditis: challenges and perspectives.","volume":"379","author":"Thuny","year":"2012","journal-title":"Lancet"},{"key":"R9-20250504","doi-asserted-by":"crossref","first-page":"310","DOI":"10.1038\/ncpcardio0535","article-title":"Infective endocarditis: diagnosis and management.","volume":"3","author":"Haldar","year":"2006","journal-title":"Nat Clin Pract Cardiovasc Med"},{"key":"R10-20250504","doi-asserted-by":"crossref","first-page":"2168","DOI":"10.1093\/eurheartj\/ehq467","article-title":"Mesenchymal stem cells improve murine acute coxsackievirus B3-induced myocarditis.","volume":"32","author":"Van Linthout","year":"2011","journal-title":"Eur Heart J"},{"key":"R11-20250504","doi-asserted-by":"crossref","first-page":"1807","DOI":"10.1056\/NEJM199306243282503","article-title":"A population-based assessment of invasive disease due to group B streptococcus in nonpregnent adults.","volume":"328","author":"Farley","year":"1993","journal-title":"N Engl J Med"},{"key":"R12-20250504","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1007\/s10096-018-3423-3","article-title":"Left-sided infective endocarditis caused by Streptococcus agalactiae: rare and serious.","volume":"38","author":"Ivanova-Georgieva","year":"2019","journal-title":"Eur J Clin Microbiol Infect Dis"},{"key":"R13-20250504","doi-asserted-by":"crossref","first-page":"38","DOI":"10.4250\/jcu.2011.19.1.38","article-title":"Infective endocarditis due to Streptococcus agalactiae in young and immunocompetent woman: a case of structurally normal valve endocarditis presented with major stroke.","volume":"19","author":"Song","year":"2011","journal-title":"J Cardiovasc Ultrasound"}],"container-title":["Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.lww.com\/10.1097\/MD.0000000000029360","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,5,4]],"date-time":"2025-05-04T04:04:34Z","timestamp":1746331474000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.lww.com\/10.1097\/MD.0000000000029360"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,7,8]]},"references-count":13,"journal-issue":{"issue":"27","published-online":{"date-parts":[[2022]]}},"URL":"https:\/\/doi.org\/10.1097\/md.0000000000029360","relation":{},"ISSN":["1536-5964"],"issn-type":[{"value":"1536-5964","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,7,8]]}}}