{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T23:43:01Z","timestamp":1787010181049,"version":"build-2736575974"},"reference-count":29,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"50","license":[{"start":{"date-parts":[[2024,12,13]],"date-time":"2024-12-13T00:00:00Z","timestamp":1734048000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2024,12,13]]},"abstract":"<jats:p>\n                    This study aimed to evaluate the diagnostic value of rapid simultaneous RNA amplification and testing for tuberculosis (SAT-TB) in smear-negative pulmonary tuberculosis (PTB). We performed a multicenter prospective analysis of 206 patients with smear-negative suspected PTB between December 2018 and March 2022. We collected sputum or bronchoalveolar lavage fluid (BALF) for simultaneous SAT-TB and Xpert\n                    <jats:italic toggle=\"yes\">Mycobacterium tuberculosis<\/jats:italic>\n                    \/rifampin (MTB\/RIF) assays. The efficiency of SAT-TB detection was also evaluated. The final analysis included 161 patients with smear-negative suspected PTB, of whom 114 provided sputum specimens and 47 provided BALF specimens. In sputum samples, the area under the curve, sensitivity, and specificity of SAT-TB for diagnosing PTB were 0.75, 50.7%, and 100.0%, respectively, and those of the Xpert MTB\/RIF assay were 0.81, 62.3%, and 100.0%, respectively. The kappa coefficient k of the consistency between SAT-TB and Xpert MTB\/RIF in sputum specimens was 0.686. In BALF specimens, the area under the curve, sensitivity, and specificity of SAT-TB for diagnosing PTB were 0.79, 57.1%, and 100.0%, respectively, and those of Xpert MTB\/RIF were 0.86, 76.2%, and 96.2%, respectively. The kappa coefficient k of the consistency between SAT-TB and Xpert MTB\/RIF in BALF specimens was 0.656. The SAT-TB and Xpert MTB\/RIF assays were highly consistent in diagnosing smear-negative PTB. It is a valuable method for early detection, prevention, and managing smear-negative PTB suspects. Meanwhile, the detection efficiency and cost-effectiveness of SAT-TB are more suitable for the rapid diagnosis of smear-negative PTB in low- and middle-income countries.\n                  <\/jats:p>","DOI":"10.1097\/md.0000000000040907","type":"journal-article","created":{"date-parts":[[2024,12,16]],"date-time":"2024-12-16T14:01:02Z","timestamp":1734357662000},"page":"e40907","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":4,"title":["Diagnostic value of SAT-TB in smear-negative pulmonary tuberculosis: A diagnostic accuracy study"],"prefix":"10.1097","volume":"103","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-0235-6035","authenticated-orcid":false,"given":"Xiaoxiao","family":"Zhao","sequence":"first","affiliation":[{"name":"Center of Infectious Diseases, West China Hospital of Sichuan University, Chengdu, Sichuan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kunping","family":"Cui","sequence":"additional","affiliation":[{"name":"Center of Infectious Diseases, West China Hospital of Sichuan University, Chengdu, Sichuan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lang","family":"Bai","sequence":"additional","affiliation":[{"name":"Center of Infectious Diseases, West China Hospital of Sichuan University, Chengdu, Sichuan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shanling","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Medical Oncology, Sichuan Cancer Hospital & Institute, Sichuan Cancer Center, School of Medicine, University of Electronic Science and Technology of China, Chengdu, Sichuan, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Liu","sequence":"additional","affiliation":[{"name":"Center of Infectious Diseases, West China Hospital of Sichuan University, Chengdu, Sichuan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin","family":"Shang","sequence":"additional","affiliation":[{"name":"Center of Infectious Diseases, West China Hospital of Sichuan University, Chengdu, Sichuan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rili","family":"Mise","sequence":"additional","affiliation":[{"name":"Center of Infectious Diseases, West China Hospital of Sichuan University, Chengdu, Sichuan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen Quan","family":"Li","sequence":"additional","affiliation":[{"name":"Infection Department, The Second People\u2019s Hospital of Yibin, Yibin, Sichuan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin","family":"Wang","sequence":"additional","affiliation":[{"name":"Infection Department, The People\u2019s Hospital of Zhongjiang, Leshan, Sichuan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen Qiu","family":"Deng","sequence":"additional","affiliation":[{"name":"Infection Department, The People\u2019s Hospital of Mianzhu, Deyang, Sichuan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lingcheng","family":"Cheng","sequence":"additional","affiliation":[{"name":"Infection Department, Armed Police Forces Hospital of Sichuan, Leshan, Sichuan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chuan","family":"Zhao","sequence":"additional","affiliation":[{"name":"Infection Department, Suining Central Hospital, Suining, Sichuan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"R1-20250503","doi-asserted-by":"crossref","first-page":"e20","DOI":"10.1016\/S2666-5247(22)00359-7","article-title":"WHO\u2019s global tuberculosis report 2022.","volume":"4","author":"Bagcchi","year":"2023","journal-title":"Lancet 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