{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,8,2]],"date-time":"2025-08-02T14:45:59Z","timestamp":1754145959181,"version":"3.41.2"},"reference-count":34,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"2","license":[{"start":{"date-parts":[[2025,1,10]],"date-time":"2025-01-10T00:00:00Z","timestamp":1736467200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Yunnan Provincial Department of Science and Technology Traditional Chinese Medicine Joint Special General Project","award":["No.202101AZ070001-090"],"award-info":[{"award-number":["No.202101AZ070001-090"]}]}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2025,1,10]]},"abstract":"<jats:sec>\n            <jats:title>Background:<\/jats:title>\n            <jats:p>Bushen-Huoxue-Mingmu-Formula (MMF) has achieved definite clinical efficacy. However, its mechanism is still unclear.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Objective:<\/jats:title>\n            <jats:p>Investigating the molecular mechanism of MMF to protect retinal ganglion cells (RGCs).<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Methods:<\/jats:title>\n            <jats:p>This study developed a pressurization-induced model of damaged RGCs, which were then treated with a serum supplemented with MMF. The effects of MMF on proliferation, apoptosis, adenosine 5\u2032-triphosphate content, and mitochondrial structure of RGCs were investigated, and the underlying molecular mechanism was explored by RNA interference experiment.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results:<\/jats:title>\n            <jats:p>In the pressurization-induced RGC injury model, apoptosis rate increased, cell proliferation decreased, adenosine 5\u2032-triphosphate content reduced, mitochondrial structure was disrupted, BCL2-associated X, cleaved caspase-3, and microtubule-associated proteins light chain 3 II\/I protein expression enhanced, B cell lymphoma-2 and p62 protein expression decreased, and the Pink1\/Parkin pathway was activated. The stress-induced damage to RGCs was, however, reversible following MMF-mediated inhibition of the Pink1\/Parkin pathway. Pink1 short-hairpin RNA downregulated Pink1 expression in RGCs, which led to outcomes that aligned with those observed with MMF intervention.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusions:<\/jats:title>\n            <jats:p>MMF altered the expression of apoptosis- and autophagy-related proteins and possibly inhibited the Pink1\/Parkin signaling pathway, which led to reduced pressurization-induced mitochondrial autophagy in RGCs. This preventive effect of MMF on RGCs can be potentially useful to preserve the viability of RGCs.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1097\/md.0000000000041257","type":"journal-article","created":{"date-parts":[[2025,1,10]],"date-time":"2025-01-10T15:01:20Z","timestamp":1736521280000},"page":"e41257","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":0,"title":["Bushen-Huoxue-Mingmu-Formula attenuates pressurization-induced retinal ganglion cell damage by reducing mitochondrial autophagy through the inhibition of the Pink1\/Parkin pathway"],"prefix":"10.1097","volume":"104","author":[{"given":"Wei","family":"Wang","sequence":"first","affiliation":[{"name":"Opthalmology, Kunming Municipal Hospital of Traditional Chinese Medicine, Kunming, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jia","family":"Gao","sequence":"additional","affiliation":[{"name":"Chengdu University of Traditional Chinese 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