{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T13:07:59Z","timestamp":1772111279325,"version":"3.50.1"},"reference-count":15,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2009,1,2]],"date-time":"2009-01-02T00:00:00Z","timestamp":1230854400000},"content-version":"vor","delay-in-days":642,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Laryngoscope"],"published-print":{"date-parts":[[2007,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:bold>Objective<\/jats:bold>: We assessed whether a nasal spray containing zinc gluconate (ZG) compromises the integrity of olfactory epithelium and olfactory function.<\/jats:p><jats:p><jats:bold>Methods<\/jats:bold>: Axonal transport of horseradish peroxidase from olfactory epithelium to the olfactory bulb was studied in 2\u2010 to 21\u2010day survival mice given intranasal injections of 2, 8, or 50 \u03bcL of ZG (approximately 4, 15, and 94 times the equivalent recommended human dose). Other similarly treated mice were tested using precision olfactometry to detect and discriminate odors.<\/jats:p><jats:p><jats:bold>Results<\/jats:bold>: Anatomic changes were graded as a function of dose and survival time. Two microliter injections had no discernable effect. While the 50 \u03bcL volume produced substantial deafferentation of input to the olfactory bulb in short\u2010survival cases. Nearly complete restitution of input occurred within 3 weeks. At each volume and survival time, zinc sulfate (ZS) had a greater effect. Behaviorally, 2 \u03bcL and 8 \u03bcL ZG\u2010treated mice and those given multiple injections of 2 \u03bcL ZG performed as well as controls, whereas those given 50 \u03bcL were hyposmic but not anosmic. ZS\u2010treated mice performed more poorly, and those injected with 50 \u03bcL were anosmic for the first 8 to 10 test days.<\/jats:p><jats:p><jats:bold>Conclusions<\/jats:bold>: A massive dose of a ZG nasal spray did cause a transient disruption of the olfactory epithelium and compromised olfaction. More moderate volumes, even those far in excess of a recommended dose, were largely without effect on odor detection and discrimination tasks. These outcomes fail to support the claims from recent clinical case reports that use of a ZG\u2010containing nasal spray can produce anosmia.<\/jats:p>","DOI":"10.1097\/mlg.0b013e318033006b","type":"journal-article","created":{"date-parts":[[2007,3,30]],"date-time":"2007-03-30T09:10:10Z","timestamp":1175245810000},"page":"743-749","source":"Crossref","is-referenced-by-count":20,"title":["Olfaction and Olfactory Epithelium in Mice Treated With Zinc Gluconate"],"prefix":"10.1002","volume":"117","author":[{"given":"Burton","family":"Slotnick","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Angelica","family":"Sanguino","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Scott","family":"Husband","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gregory","family":"Marquino","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alan","family":"Silberberg","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,1,2]]},"reference":[{"key":"e_1_2_6_2_2","first-page":"34","volume-title":"Polio and its Problems","author":"Berg RH","year":"1948"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1093\/chemse\/bjg053"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1037\/0735-7044.114.4.814"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.mlg.0000191549.17796.13"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1177\/194589240401800302"},{"key":"e_1_2_6_7_2","first-page":"32","article-title":"The effect of zinc sulphate on the sense of smell.","volume":"3","author":"Echols DH","year":"1937","journal-title":"Univ Hospital Bull"},{"key":"e_1_2_6_8_2","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1016\/S0025-7125(16)37080-8","article-title":"Zinc sulfate intranasal spray technic in poliomyelitis prevention.","volume":"22","author":"Hayden AA","year":"1938","journal-title":"Med Clin North Am"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1001\/jama.1937.02780260012004"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/70.1.7"},{"key":"e_1_2_6_11_2","first-page":"523","article-title":"Zinc\u2010sulfate nasal spray in the prophylaxis of poliomyelitis.","volume":"28","author":"Tisdall FF","year":"1937","journal-title":"Can Public Health J"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1590\/S0034-72992005000400014"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1001\/archotol.1991.01870240094016"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1001\/jama.1938.02790160012004"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0001-6519(06)78677-0"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00204-002-0321-2"}],"container-title":["The Laryngoscope"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1097%2FMLG.0b013e318033006b","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1097\/MLG.0b013e318033006b","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,15]],"date-time":"2023-11-15T13:11:29Z","timestamp":1700053889000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1097\/MLG.0b013e318033006b"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,4]]},"references-count":15,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2007,4]]}},"alternative-id":["10.1097\/MLG.0b013e318033006b"],"URL":"https:\/\/doi.org\/10.1097\/mlg.0b013e318033006b","archive":["Portico"],"relation":{},"ISSN":["0023-852X","1531-4995"],"issn-type":[{"value":"0023-852X","type":"print"},{"value":"1531-4995","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,4]]}}}