{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,11,15]],"date-time":"2023-11-15T11:42:36Z","timestamp":1700048556472},"reference-count":14,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2009,1,2]],"date-time":"2009-01-02T00:00:00Z","timestamp":1230854400000},"content-version":"vor","delay-in-days":612,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Laryngoscope"],"published-print":{"date-parts":[[2007,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:bold>Hypothesis<\/jats:bold>: The main goal of this study was to perform an immunohistologic analysis of bone morphogenetic protein receptors (BMPR) in otospongiosis.<\/jats:p><jats:p><jats:bold>Background.<\/jats:bold> BMP\u20102, \u20104, and \u20107 play an essential role in bone formation and repair. They do so as well in otosclerosis. It has been shown that these BMPs are traceable in osteocytes and osteoclasts in the active phase of otosclerosis (otospongiosis). The role of the different BMP receptors in otosclerotic bone transformation has not been previously analyzed.<\/jats:p><jats:p><jats:bold>Methods<\/jats:bold>: The posterior parts of the stapes footplates, collected during partial stapedectomies in 35 patients with clinical otosclerosis, were analyzed for histologic otosclerotic lesions after hematoxylin staining. Immunohistochemical analysis was performed using polyclonal immunoglobulin G antibodies for BMPR\u2010IA, \u2010IB, and \u2010II, as well as biotinylated secondary antibodies, avidin\u2010biotin\u2010peroxidase complex reaction, and alkaline phosphatase staining with nitroblue\u2010tetrazolium\u2010chloride.<\/jats:p><jats:p><jats:bold>Results<\/jats:bold>: Seventeen of 35 (49%) specimens contained otosclerosis, but only 5 of these exhibited an otospongiotic phase. The abundant osteoblasts and osteoclasts in these cases showed distinct immunochemical staining for BMP\u20102, \u20104, and \u20107. In two cases, there could also be found an immense positive staining for BMPR\u2010IB and modest staining for BMPR\u2010II, whereas BMPR\u20101A always remained negative.<\/jats:p><jats:p><jats:bold>Conclusion<\/jats:bold>: It was demonstrated for the first time that in otospongiosis, the actions of the BMPs are mediated through BMPR\u2010IB and BMPR\u2010II. To determine this role in detail, further investigations, especially for the phosphorylated Smad proteins within the BMP dependent mediator cascade, will be necessary.<\/jats:p>","DOI":"10.1097\/mlg.0b013e31803300a2","type":"journal-article","created":{"date-parts":[[2007,4,18]],"date-time":"2007-04-18T08:04:08Z","timestamp":1176883448000},"page":"812-816","source":"Crossref","is-referenced-by-count":10,"title":["Signaling by Way of Type IB and II Bone Morphogenetic Protein Receptors Regulates Bone Formation in Otospongiosis"],"prefix":"10.1002","volume":"117","author":[{"given":"Goetz","family":"Lehnerdt","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Klaus Alfred","family":"Metz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sokratis","family":"Trellakis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Klaus","family":"Jahnke","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andreas","family":"Neumann","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,1,2]]},"reference":[{"key":"e_1_2_5_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.1289"},{"key":"e_1_2_5_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.20799"},{"key":"e_1_2_5_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S8756-3282(96)00259-1"},{"key":"e_1_2_5_5_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M300969200"},{"key":"e_1_2_5_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.10258"},{"key":"e_1_2_5_7_2","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.2002.0822"},{"key":"e_1_2_5_8_2","first-page":"243","article-title":"Signaling via type IA and type IB bone morphogenetic protein receptors (BMPR) regulates intramembranous bone formation, chondrogenesis and feather formation in the chicken embryo.","volume":"46","author":"Ashique AM","year":"2002","journal-title":"Int J Dev Biol"},{"key":"e_1_2_5_9_2","doi-asserted-by":"publisher","DOI":"10.1177\/000348940010900106"},{"key":"e_1_2_5_10_2","first-page":"15","volume-title":"Otosclerosis","author":"Altman F","year":"1962"},{"key":"e_1_2_5_11_2","doi-asserted-by":"publisher","DOI":"10.2106\/00004623-200211000-00001"},{"key":"e_1_2_5_12_2","first-page":"2339","article-title":"Immunohistochemical localization of bone morphogenetic proteins and the receptors in epiphyseal growth plate.","volume":"18","author":"Yuzaki Y","year":"1998","journal-title":"Anticancer Res"},{"key":"e_1_2_5_13_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.142.1.295"},{"key":"e_1_2_5_14_2","doi-asserted-by":"crossref","first-page":"1483","DOI":"10.1242\/jcs.114.8.1483","article-title":"Synergistic effects of different bone morphogenetic protein type I receptors on alkaline phosphatase induction.","volume":"114","author":"Aoki H","year":"2001","journal-title":"J Cell Sci"},{"key":"e_1_2_5_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocel.2006.05.005"}],"container-title":["The Laryngoscope"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1097%2FMLG.0b013e31803300a2","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1097\/MLG.0b013e31803300a2","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,15]],"date-time":"2023-11-15T11:15:56Z","timestamp":1700046956000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1097\/MLG.0b013e31803300a2"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,5]]},"references-count":14,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2007,5]]}},"alternative-id":["10.1097\/MLG.0b013e31803300a2"],"URL":"https:\/\/doi.org\/10.1097\/mlg.0b013e31803300a2","archive":["Portico"],"relation":{},"ISSN":["0023-852X","1531-4995"],"issn-type":[{"value":"0023-852X","type":"print"},{"value":"1531-4995","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,5]]}}}