{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T03:51:05Z","timestamp":1789962665034,"version":"4.0.1"},"reference-count":18,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2009,1,2]],"date-time":"2009-01-02T00:00:00Z","timestamp":1230854400000},"content-version":"vor","delay-in-days":489,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Laryngoscope"],"published-print":{"date-parts":[[2007,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:bold>Objective:<\/jats:bold> The loss of spiral ganglion neurons (SGNs) is one of the major causes of profound sensorineural hearing loss (SNHL). Stem cell replacement therapy, which is still in its infancy, has the potential to treat or cure those who suffer from an array of illnesses and degenerative neurologic disorders, including sensorineural deafness (SNHL). Little is known about the potentials of mesenchymal stem cells (MSCs) and their ability to take on properties of SGNs. The two main purposes of this study were to evaluate the survival of mouse MSCs transplanted into normal and ouabain\u2010treated gerbil cochleae and to determine the migratory patterns of MSCs with two differing injection methods.<\/jats:p><jats:p><jats:bold>Subjects:<\/jats:bold> Thirty\u2010two Mongolian gerbils, 3 to 4 months old, were used as recipients, and four 6\u2010week\u2010old TgN(ACTbEGFP) mice that ubiquitously express green fluorescent protein (GFP) were used as donors.<\/jats:p><jats:p><jats:bold>Design:<\/jats:bold> The animals were deafened by ouabain, which damaged SGNs while leaving hair cell systems intact. After 4 weeks of recovery, the animals received an intraperilymphatic transplantation of 1.0 \u00d7 10<jats:sup>6<\/jats:sup> GFP\u2010positive undifferentiated MSCs via two different injection methods: scala tympani injection and modiolar injection. Seven days after the transplantation, the survival of MSCs was evaluated by microscopic examination of frozen sections cut through the cochleae of the recipient animals. The number of profiles was counted on the five most central modiolar sections. One\u2010way analyses of variance (ANOVA) were used to determine any significantdifferences among mean profile counts across the experimental conditions.<\/jats:p><jats:p><jats:bold>Results:<\/jats:bold> Our findings indicated that undifferentiated MSCs were able to survive in the modiolus both in the control and the ouabain\u2010treated cochleae. The average number of profiles found in the modiolus was greater in the ouabain\u2010treated cochleae than in the control cochleae. This difference was statistically significant (<jats:italic>P<\/jats:italic> &lt; .01) as determined using a one\u2010way ANOVA and an ad hoc Tukey\u2010Kramer's test. With the scala tympani injection, there were no profiles found in the modiolus either in the control or ouabain\u2010treated cochleae. This finding may indicate that donor MSCs need to be directly injected into the modiolus to replace injured SGNs. Finally, there was no evidence of hyperacute rejection in any of the gerbils despite the use of xenotransplantation.<\/jats:p><jats:p><jats:bold>Conclusions:<\/jats:bold> These findings may have important clinical implications as a means of delivering MSCs in the cochlea for stem\u2010cell replacement therapy. Survival of transplanted MSCs into the modiolus of the cochlea may result in regeneration of damaged SGNs.<\/jats:p>","DOI":"10.1097\/mlg.0b013e31806bf282","type":"journal-article","created":{"date-parts":[[2007,7,13]],"date-time":"2007-07-13T08:50:32Z","timestamp":1184316632000},"page":"1629-1635","source":"Crossref","is-referenced-by-count":62,"title":["Enhanced Survival of Bone\u2013Marrow\u2010Derived Pluripotent Stem Cells in an Animal Model of Auditory Neuropathy"],"prefix":"10.1002","volume":"117","author":[{"given":"Akihiro J.","family":"Matsuoka","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takako","family":"Kondo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard T.","family":"Miyamoto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eri","family":"Hashino","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2009,1,2]]},"reference":[{"key":"e_1_2_7_2_2","first-page":"799","article-title":"Studies on the physiological role of brain\u2010derived neurotrophic factor and neurotrophin\u20103 in knockout mice","volume":"39","author":"Ernfors P","year":"1995","journal-title":"Int J Dev Biol"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1177\/000348949810701102"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.2001.4570"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1097\/00001756-200401190-00001"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.mlg.0000225986.18790.75"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1007\/s1016200220017"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0022215100089696"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1432929100"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0408239102"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-5955(99)00193-8"},{"key":"e_1_2_7_12_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9861(19960101)364:1<6::AID-CNE2>3.0.CO;2-9"},{"key":"e_1_2_7_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/35097581"},{"key":"e_1_2_7_14_2","first-page":"4268","article-title":"Incorporation and differentiation of hippocampus\u2010derived neural stem cells transplanted in injured adult rat retina","volume":"41","author":"Nishida A","year":"2000","journal-title":"IOVS"},{"key":"e_1_2_7_15_2","first-page":"63","volume-title":"CNS Injuries: Cellular Responses and Pharmacological Strategies.","author":"Brown K","year":"1999"},{"key":"e_1_2_7_16_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.032677999"},{"key":"e_1_2_7_17_2","doi-asserted-by":"publisher","DOI":"10.1002\/neu.20310"},{"key":"e_1_2_7_18_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.288.5471.1660"},{"key":"e_1_2_7_19_2","first-page":"85","article-title":"The canaliculae perforantes of Schuknecht","volume":"31","author":"Lim DJ","year":"1983","journal-title":"Adv Otorhinolaryngol"}],"container-title":["The Laryngoscope"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1097%2FMLG.0b013e31806bf282","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1097\/MLG.0b013e31806bf282","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,9]],"date-time":"2023-10-09T22:28:13Z","timestamp":1696890493000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1097\/MLG.0b013e31806bf282"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,9]]},"references-count":18,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2007,9]]}},"alternative-id":["10.1097\/MLG.0b013e31806bf282"],"URL":"https:\/\/doi.org\/10.1097\/mlg.0b013e31806bf282","archive":["Portico"],"relation":{},"ISSN":["0023-852X","1531-4995"],"issn-type":[{"value":"0023-852X","type":"print"},{"value":"1531-4995","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,9]]}}}