{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T18:14:13Z","timestamp":1791137653126,"version":"4.1.0"},"reference-count":22,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2009,1,2]],"date-time":"2009-01-02T00:00:00Z","timestamp":1230854400000},"content-version":"vor","delay-in-days":367,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Laryngoscope"],"published-print":{"date-parts":[[2008,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:bold>Objectives:<\/jats:bold> After axotomy, Schwann cells (SCs), required for successful nerve regeneration, undergo a number of cellular changes including dedifferentiation, proliferation, expression of molecules that support axon growth, and apoptosis. This study investigated the role of p75<jats:sup>NTR<\/jats:sup>, sortilin, and proneurotrophins in SC survival after facial nerve (FN) axotomy.<\/jats:p><jats:p><jats:bold>Study Design:<\/jats:bold> Preliminary animal study.<\/jats:p><jats:p><jats:bold>Methods:<\/jats:bold> With use of FN SCs, expression of p75<jats:sup>NTR<\/jats:sup> and its coreceptor sortilin were quantified by immunofluorescence on days 12, 22, and 52 after axotomy in vivo and by Western blot in vitro. Contralateral FNs served as a control. SC apoptosis was detected by terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL). To verify a causative role for p75<jats:sup>NTR<\/jats:sup> in FN SC death, cultured FN SCs were treated with pro\u2010nerve growth factor (NGF), and apoptosis was determined by TUNEL.<\/jats:p><jats:p><jats:bold>Results:<\/jats:bold> Expression of p75<jats:sup>NTR<\/jats:sup> and sortilin increased in FN SCs distal (<jats:italic>P<\/jats:italic> &lt; .05) to the axotomy compared with the contralateral controls for all time points. SC apoptosis also significantly increased in the distal segment compared with the contralateral and proximal portions (<jats:italic>P<\/jats:italic> &lt; .05). ProNGF, a p75<jats:sup>NTR<\/jats:sup> ligand, increased apoptosis and p75<jats:sup>NTR<\/jats:sup> expression in primary FN SC cultures.<\/jats:p><jats:p><jats:bold>Conclusion:<\/jats:bold> FN axotomy increases p75<jats:sup>NTR<\/jats:sup> and sortilin expression in SCs, which correlates with increased apoptosis. These findings suggest roles for p75<jats:sup>NTR<\/jats:sup> and sortilin in SC loss after FN injury. Sortilin is a novel target in promoting FN healing after injury.<\/jats:p>","DOI":"10.1097\/mlg.0b013e31814b8d9f","type":"journal-article","created":{"date-parts":[[2009,2,19]],"date-time":"2009-02-19T21:46:23Z","timestamp":1235079983000},"page":"87-93","source":"Crossref","is-referenced-by-count":29,"title":["p75NTR and Sortilin Increase After Facial Nerve Injury"],"prefix":"10.1002","volume":"118","author":[{"given":"Matthew J.","family":"Provenzano","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ningyong","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark R.","family":"Ver Meer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J Jason","family":"Clark","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marlan R.","family":"Hansen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2009,1,2]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.otc.2006.04.002"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0301-0082(99)00049-0"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.neuro.30.051606.094337"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(02)03035-4"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-3940(99)00618-7"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4695(199801)34:1<1::AID-NEU1>3.0.CO;2-C"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(90)90107-Q"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrn1078"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/glia.1113"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.1093\/jnen\/62.4.398"},{"key":"e_1_2_7_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2004.04.001"},{"key":"e_1_2_7_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(02)01005-X"},{"key":"e_1_2_7_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature02319"},{"key":"e_1_2_7_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-5955(01)00267-2"},{"key":"e_1_2_7_16_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1750-3639.2006.00035.x"},{"key":"e_1_2_7_17_2","doi-asserted-by":"publisher","DOI":"10.1007\/s007010170114"},{"key":"e_1_2_7_18_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.7.2795"},{"key":"e_1_2_7_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/0166-2236(88)90090-2"},{"key":"e_1_2_7_20_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.19-12-04828.1999"},{"key":"e_1_2_7_21_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.embor.7400219"},{"key":"e_1_2_7_22_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.08-02-00664.1988"},{"key":"e_1_2_7_23_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.6.3599"}],"container-title":["The Laryngoscope"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1097%2FMLG.0b013e31814b8d9f","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1097\/MLG.0b013e31814b8d9f","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,14]],"date-time":"2023-11-14T13:35:18Z","timestamp":1699968918000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1097\/MLG.0b013e31814b8d9f"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,1]]},"references-count":22,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2008,1]]}},"alternative-id":["10.1097\/MLG.0b013e31814b8d9f"],"URL":"https:\/\/doi.org\/10.1097\/mlg.0b013e31814b8d9f","archive":["Portico"],"relation":{},"ISSN":["0023-852X","1531-4995"],"issn-type":[{"value":"0023-852X","type":"print"},{"value":"1531-4995","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,1]]}}}