{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T02:12:42Z","timestamp":1792807962035,"version":"4.5.0"},"reference-count":32,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2009,1,2]],"date-time":"2009-01-02T00:00:00Z","timestamp":1230854400000},"content-version":"vor","delay-in-days":154,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Laryngoscope"],"published-print":{"date-parts":[[2008,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:bold>Objectives\/Hypothesis:<\/jats:bold> Determine the role of estrogen receptor (ER) and progesterone receptor (PR) expression in sporadic and neurofibromatosis 2 (NF2)\u2010related vestibular schwannomas (VS). Growth and proliferation signaling in human VS tumorigenesis may play a key role in molecular therapeutic targeting. VS carry mutations of the NF2 gene encoding the tumor suppressor, merlin, which interacts with ErbB2 in Schwann cells, implicating ErbB receptors in VS tumorigenesis. ErbB receptor family members are overexpressed or constitutively activated in many human tumors, and are effective therapeutic targets in some human cancers. VS occur more frequently in women and are larger, more vascular, and demonstrate increased growth rates during pregnancy. ER and PR may play a role in ErbB pathway activation and VS progression.<\/jats:p><jats:p><jats:bold>Study Design:<\/jats:bold> Quantitative real\u2010time polymerase chain reaction (qRT\u2010PCR) for ER and PR messenger RNA was performed using greater auricular and vestibular nerve controls (n = 8), sporadic VS (n = 23), and NF2\u2010related VS (n = 16) tissues.<\/jats:p><jats:p><jats:bold>Methods:<\/jats:bold> The qRT\u2010PCR data were normalized with standardization to a single constitutively expressed control gene, human cyclophylin.<\/jats:p><jats:p><jats:bold>Results:<\/jats:bold> Reverse transcription of messenger RNA from control and tumor specimens followed by RT Q\u2010PCR demonstrated differences in ER and PR gene expression between sporadic and NF2\u2010related VS.<\/jats:p><jats:p><jats:bold>Conclusions:<\/jats:bold> ER and PR expression in VS might have implications for development of a VS\u2010specific drug delivery system using antihormone and ErbB pathway small molecule inhibitors, due to crosstalk between these receptors. These signals may be critical for re\u2010establishing ErbB\u2010mediated cell density dependent growth inhibition.<\/jats:p>","DOI":"10.1097\/mlg.0b013e318177e20b","type":"journal-article","created":{"date-parts":[[2009,2,19]],"date-time":"2009-02-19T21:23:14Z","timestamp":1235078594000},"page":"1458-1463","source":"Crossref","is-referenced-by-count":26,"title":["Vestibular Schwannoma Quantitative Polymerase Chain Reaction Expression of Estrogen and Progesterone Receptors"],"prefix":"10.1002","volume":"118","author":[{"given":"Andrew K.","family":"Patel","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas H.","family":"Alexander","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ali","family":"Andalibi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Allen F.","family":"Ryan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joni K.","family":"Doherty","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2009,1,2]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1097\/00005537-200004000-00002"},{"key":"e_1_2_7_3_2","doi-asserted-by":"crossref","first-page":"499","DOI":"10.1016\/S0030-6665(20)30962-2","article-title":"Epidemiology, pathogenesis, and genetics of acoustic tumors.","volume":"25","author":"Lanser MJ","year":"1992","journal-title":"Otolaryngol Clin North Am"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1097\/00129492-200501000-00016"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1212\/01.WNL.0000163982.78900.AD"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1288\/00005537-199507000-00005"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-0173(01)00139-4"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1097\/00129492-200403000-00013"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01411055"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00168034"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.3171\/jns.1980.52.1.0028"},{"key":"e_1_2_7_12_2","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0412.2001.080002179.x"},{"key":"e_1_2_7_13_2","first-page":"25","article-title":"Pregnancy and neurofibromatosis (vonRecklinghausen's disease).","volume":"47","author":"Ansari AH","year":"1976","journal-title":"Obstet Gynecol"},{"key":"e_1_2_7_14_2","volume-title":"Tumors of the Nervus Acusticus and the Syndrome of the Cerebello\u2010pontine Angle","author":"Cushing H","year":"1917"},{"key":"e_1_2_7_15_2","doi-asserted-by":"publisher","DOI":"10.1007\/s007010170063"},{"key":"e_1_2_7_16_2","doi-asserted-by":"publisher","DOI":"10.1097\/00002826-198412000-00015"},{"key":"e_1_2_7_17_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0022215100114197"},{"key":"e_1_2_7_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/0090-3019(86)90366-6"},{"key":"e_1_2_7_19_2","doi-asserted-by":"publisher","DOI":"10.1001\/archotol.1990.01870020078020"},{"key":"e_1_2_7_20_2","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1227\/00006123-198808000-00010","article-title":"Steroid receptors in human brain and spinal tumors.","volume":"23","author":"Kornblum JA","year":"1988","journal-title":"Neurosurgery"},{"key":"e_1_2_7_21_2","first-page":"303","article-title":"Sex hormone receptors in intracranial tumours and normal brain.","volume":"13","author":"Whittle IR","year":"1987","journal-title":"Eur J Surg Oncol"},{"key":"e_1_2_7_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/0090-3019(81)90023-9"},{"key":"e_1_2_7_23_2","doi-asserted-by":"publisher","DOI":"10.1097\/mao.0b013e31815d4429"},{"key":"e_1_2_7_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/0090-3019(83)90201-X"},{"key":"e_1_2_7_25_2","doi-asserted-by":"publisher","DOI":"10.1097\/MOO.0b013e3282b97310"},{"key":"e_1_2_7_26_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2273.1995.tb00073.x"},{"key":"e_1_2_7_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9378(74)90212-9"},{"key":"e_1_2_7_28_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0022215107000229"},{"key":"e_1_2_7_29_2","doi-asserted-by":"publisher","DOI":"10.1097\/00002826-198412000-00011"},{"key":"e_1_2_7_30_2","doi-asserted-by":"crossref","first-page":"665","DOI":"10.1227\/00006123-198112000-00009","article-title":"Specific estradiol binding in schwannomas, meningiomas, and neurofibromas.","volume":"9","author":"Martuza RL","year":"1981","journal-title":"Neurosurgery"},{"key":"e_1_2_7_31_2","doi-asserted-by":"publisher","DOI":"10.1097\/00005537-199606000-00007"},{"key":"e_1_2_7_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/0090-3019(89)90005-0"},{"key":"e_1_2_7_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-0760(97)00191-X"}],"container-title":["The Laryngoscope"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1097%2FMLG.0b013e318177e20b","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1097\/MLG.0b013e318177e20b","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,10]],"date-time":"2023-10-10T09:42:21Z","timestamp":1696930941000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1097\/MLG.0b013e318177e20b"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,8]]},"references-count":32,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2008,8]]}},"alternative-id":["10.1097\/MLG.0b013e318177e20b"],"URL":"https:\/\/doi.org\/10.1097\/mlg.0b013e318177e20b","archive":["Portico"],"relation":{},"ISSN":["0023-852X","1531-4995"],"issn-type":[{"value":"0023-852X","type":"print"},{"value":"1531-4995","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,8]]}}}