{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,17]],"date-time":"2025-09-17T15:32:21Z","timestamp":1758123141098},"reference-count":42,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2009,1,2]],"date-time":"2009-01-02T00:00:00Z","timestamp":1230854400000},"content-version":"vor","delay-in-days":123,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Laryngoscope"],"published-print":{"date-parts":[[2008,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:bold>Objectives\/Hypothesis:<\/jats:bold> To examine the level of expression of galectin\u20103 in relation to neoplastic progression of hypopharyngeal squamous cell carcinomas (HSCCs) and laryngeal squamous cell carcinomas (LSCCs).<\/jats:p><jats:p><jats:bold>Study Design:<\/jats:bold> Retrospective study.<\/jats:p><jats:p><jats:bold>Methods:<\/jats:bold> Using a polyclonal antibody against galectin\u20103 without cross\u2010reactivity to other galectins, we analyzed the presence of galectin\u20103 using quantitative immunohistochemistry in i) a series of 79 HSCCs compared with 16 normal epithelia, 20 low\u2010grade dysplasia (Low_D) and 25 high\u2010grade dysplasia (High_D) and in ii) a series of 58 LSCCs compared with 34 normal epithelia, 12 Low_D, and 18 High_D. In parallel, galectin\u20103 expression was studied using Western blotting on a series of 19 fresh biopsies from patients presenting a head and neck tumor.<\/jats:p><jats:p><jats:bold>Results:<\/jats:bold> Western blotting excluded a notable degree of proteolytic truncation of galectin\u20103 in situ. Immunohistochemical galectin\u20103 positivity expressed as percentage of cells was significantly higher in LSCCs and HSCCs than in Low_D (<jats:italic>P<\/jats:italic> = .01) or High_D (<jats:italic>P<\/jats:italic> = .0002), respectively. Increased expression of galectin\u20103 in HSCCs was accompanied by a shift from the cytoplasmic compartment to the nucleus (<jats:italic>P<\/jats:italic> = .007). In intertumor\u2010type comparison, laryngeal carcinomas presented nuclear presence of galectin\u20103 only rarely (1 of 58 cases in laryngeal cancer vs. 27 of 79 cases in hypopharyngeal cancer, <jats:italic>P<\/jats:italic> = .00006) and a comparatively low labeling index (<jats:italic>P<\/jats:italic> &lt; 10<jats:sup>\u22126<\/jats:sup>).<\/jats:p><jats:p><jats:bold>Conclusions:<\/jats:bold> Our data reveal an association between level of presence of galectin\u20103 and neoplastic progression of HSCCs and LSCCs.<\/jats:p>","DOI":"10.1097\/mlg.0b013e31817b0718","type":"journal-article","created":{"date-parts":[[2008,8,27]],"date-time":"2008-08-27T07:18:27Z","timestamp":1219821507000},"page":"1583-1590","source":"Crossref","is-referenced-by-count":29,"title":["Galectin\u20103 Upregulation During Tumor Progression in Head and Neck Cancer"],"prefix":"10.1002","volume":"118","author":[{"given":"Sven","family":"Saussez","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christine","family":"Decaestecker","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Virginie","family":"Mahillon","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"St\u00e9phanie","family":"Cludts","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aur\u00e9lie","family":"Capouillez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dominique","family":"Chevalier","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Herbert Kaltner","family":"Vet","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sabine","family":"Andr\u00e9","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G\u00e9rard","family":"Toubeau","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xavier","family":"Leroy","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hans\u2010Joachim","family":"Gabius","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,1,2]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.3109\/07357909709047585"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1742-4658.2007.05851.x"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.2174\/0929867003375164"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1615\/CritRevImmunol.v26.i1.30"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.4052\/tigg.18.1"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagen.2004.03.013"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00418-005-0074-0"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.13.6737"},{"key":"e_1_2_7_10_2","first-page":"5272","article-title":"Galectin\u20103: a novel antiapoptotic molecule with a functional BH1 (NWGR) domain of Bcl\u20102 family.","volume":"57","author":"Akahani S","year":"1997","journal-title":"Cancer Res"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.1007\/s004320050303"},{"key":"e_1_2_7_12_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M105135200"},{"key":"e_1_2_7_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1205820"},{"key":"e_1_2_7_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-291X(03)00151-7"},{"key":"e_1_2_7_15_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj20030786"},{"key":"e_1_2_7_16_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10863-006-9063-9"},{"key":"e_1_2_7_17_2","first-page":"2131","article-title":"Growth\/adhesion\u2010regulatory tissue lectin galectin\u20103: stromal presence but not cytoplasmic\/nuclear expression in tumor cells as negative prognostic factor in breast cancer.","volume":"27","author":"Moisa A","year":"2007","journal-title":"Anticancer Res"},{"key":"e_1_2_7_18_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0347(199609\/10)18:5<422::AID-HED5>3.0.CO;2-7"},{"key":"e_1_2_7_19_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0142(19991201)86:11<2353::AID-CNCR25>3.0.CO;2-A"},{"key":"e_1_2_7_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0194-59980070010-6"},{"key":"e_1_2_7_21_2","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2002.01.078"},{"key":"e_1_2_7_22_2","doi-asserted-by":"publisher","DOI":"10.1002\/hed.10344"},{"key":"e_1_2_7_23_2","volume-title":"Tumors of the Upper Respiratory Tract and Ear. 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