{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,18]],"date-time":"2026-01-18T05:25:57Z","timestamp":1768713957917,"version":"3.49.0"},"reference-count":32,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2007,9,1]],"date-time":"2007-09-01T00:00:00Z","timestamp":1188604800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100012553","name":"Chang Gung Memorial Hospital","doi-asserted-by":"publisher","award":["CMRPG850281"],"award-info":[{"award-number":["CMRPG850281"]}],"id":[{"id":"10.13039\/100012553","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. pediatr. gastroenterol. nutr."],"published-print":{"date-parts":[[2007,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Objectives:<\/jats:title><jats:p>Oxidative stress is known to be involved in the pathogenesis of biliary atresia (BA), but the mechanism has yet to be elucidated. We studied 8\u2010hydroxydeoxyguanosine (8\u2010OHdG) and mitochondrial copy number as potential markers for oxidative stress in BA.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods:<\/jats:title><jats:p>Hepatic immunoreactive 8\u2010OHdG expression was investigated during the early stage of BA when the patients received Kasai portoenterostomy (KP), during the late stage when the patients received liver transplantation (LT), in patients with choledochal cyst as disease control, and in patients with histologically normal liver as normal control. Apoptosis of liver cells was examined by terminal deoxynucleotidyl transferase\u2013mediated 2\u2032\u2010deoxyuridine 5\u2032\u2010triphosphate\u2010biotin nick end\u2010labeling stain. The mitochondrial DNA copy number was measured by real\u2010time polymerase chain reaction.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results:<\/jats:title><jats:p>The number of hepatocytes positive for immunoreactive 8\u2010OHdG was significantly increased in KP (65% \u00b1 18%) compared with LT (30% \u00b1 32%; <jats:italic>P<\/jats:italic> = 0.029) and choledochal cyst (25% \u00b1 20%; <jats:italic>P<\/jats:italic> = 0.037). The 8\u2010OHdG labeling index was significantly correlated with the grade of chronic hepatitis activity (Spearman <jats:italic>r<\/jats:italic> = 0.495; <jats:italic>P<\/jats:italic> = 0.037). The hepatocyte terminal deoxynucleotidyl transferase\u2013mediated 2\u2032\u2010deoxyuridine 5\u2032\u2010triphosphate\u2010biotin nick end\u2010labeling index in KP (15% \u00b1 4%) was significantly higher than that in LT (5% \u00b1 2%; <jats:italic>P<\/jats:italic> = 0.018) and in choledochal cyst (3% \u00b1 2%; <jats:italic>P<\/jats:italic> = 0.010). Mitochondrial copy number was significantly less in KP than in LT (7.33 \u00b1 0.75 vs 8.91 \u00b1 1.32; <jats:italic>P<\/jats:italic> = 0.045) and in normal control (7.33 \u00b1 0.75 vs 9.20 \u00b1 1.20; <jats:italic>P<\/jats:italic> = 0.021).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions:<\/jats:title><jats:p>The early stage of BA is associated with stronger inflammatory reaction, augmented oxidative DNA, and mitochondrial DNA damage as manifested by higher immunoreactive 8\u2010OHdG and apoptotic activities and by a decrease in mitochondrial copy number.<\/jats:p><\/jats:sec>","DOI":"10.1097\/mpg.0b013e3180cc2c0f","type":"journal-article","created":{"date-parts":[[2009,3,4]],"date-time":"2009-03-04T22:08:15Z","timestamp":1236204495000},"page":"329-334","source":"Crossref","is-referenced-by-count":43,"title":["Early Stage of Biliary Atresia Is Associated With Significant Changes in 8\u2010Hydroxydeoxyguanosine and Mitochondrial Copy Number"],"prefix":"10.1002","volume":"45","author":[{"given":"Mao\u2010Meng","family":"Tiao","sequence":"first","affiliation":[{"name":"Department of Pediatrics Chang Gung Memorial Hospital\u2010Kaohsiung Medical Center Chang Gung University College of Medicine Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tsu\u2010Kung","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Neurology Chang Gung Memorial Hospital\u2010Kaohsiung Medical Center Chang Gung University College of Medicine Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fang\u2010Ying","family":"Kuo","sequence":"additional","affiliation":[{"name":"Department of Pathology Chang Gung Memorial Hospital\u2010Kaohsiung Medical Center Chang Gung University College of Medicine Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chao\u2010Cheng","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Pathology Chang Gung Memorial Hospital\u2010Kaohsiung Medical Center Chang Gung University College of Medicine Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yung\u2010Ying","family":"Du","sequence":"additional","affiliation":[{"name":"Department of Pediatric Surgery Chang Gung Memorial Hospital\u2010Kaohsiung Medical Center Chang Gung University College of Medicine Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chao\u2010Long","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Surgery Chang Gung Memorial Hospital\u2010Kaohsiung Medical Center Chang Gung University College of Medicine Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiin\u2010Haur","family":"Chuang","sequence":"additional","affiliation":[{"name":"Department of Pediatric Surgery Chang Gung Memorial Hospital\u2010Kaohsiung Medical Center Chang Gung University College of Medicine 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