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Milk consumption, body weight, fecal consistency, and oocyst excretion were recorded daily. On day 3 postinfection, piglets were euthanized and serum concentration of NO metabolites and histological severity of villous atrophy and epithelial infection were quantified. Sheets of ileal mucosa were mounted in Ussing chambers for measurement of barrier function (transepithelial resistance and permeability) and short\u2010circuit current (an indirect measurement of Cl<jats:sup>\u2212<\/jats:sup>secretion in this tissue).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results:<\/jats:title><jats:p><jats:italic>C parvum<\/jats:italic>\u2013infected piglets had large numbers of epithelial parasites, villous atrophy, decreased barrier function, severe watery diarrhea, and failure to gain weight. L\u2010Arginine promoted synthesis of NO by infected piglets, which was unaccompanied by improvement in severity of infection but rather promoted epithelial chloride secretion and diarrhea. Epithelial secretion by infected mucosa from L\u2010arginine\u2010supplemented piglets was fully inhibited by the cyclooxygenase inhibitor indomethacin, indicating that prostaglandin synthesis was responsible for this effect.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions:<\/jats:title><jats:p>Results of these studies demonstrate that provision of additional NO substrate in the form of L\u2010arginine incites prostaglandin\u2010dependent secretory diarrhea and does not promote epithelial defense or barrier function of<jats:italic>C parvum<\/jats:italic>\u2013infected neonatal ileum.<\/jats:p><\/jats:sec>","DOI":"10.1097\/mpg.0b013e31815c0480","type":"journal-article","created":{"date-parts":[[2009,3,5]],"date-time":"2009-03-05T06:28:40Z","timestamp":1236234520000},"page":"139-146","source":"Crossref","is-referenced-by-count":11,"title":["Oral Delivery of L\u2010arginine Stimulates Prostaglandin\u2010dependent Secretory Diarrhea in<i>Cryptosporidium parvum<\/i>\u2013infected 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