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Epithelial expression of L\u2010arginine transporter genes was quantified by real\u2010time reverse transcription polymerase chain reaction. L\u2010Arginine catabolic enzyme expression was examined by immunoblotting epithelial lysates for arginase I and II. The role of intracellular catabolism in promoting the uptake of L\u2010arginine was determined by pharmacological inhibition of nitric oxide synthase and arginase activities.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results:<\/jats:title><jats:p><jats:italic>C parvum<\/jats:italic>\u2013infected ileum transported L\u2010arginine at rates equivalent to uninfected epithelium despite profound villous atrophy. This was attributed to enhanced uptake of L\u2010arginine by individual epithelial cells in the infection. There were no differences in L\u2010arginine transport system activities (y<jats:sup>+<\/jats:sup>and B<jats:sup>0,+<\/jats:sup>) or level of transporter gene expression (CAT\u20101, CAT\u20102A, and ATB<jats:sup>0,+<\/jats:sup>) between uninfected and<jats:italic>C parvum<\/jats:italic>\u2013infected epithelial cells. However, infected epithelia had induced expression of the L\u2010arginine hydrolytic enzyme arginase II and lower concentrations of L\u2010arginine. Furthermore, transport of L\u2010arginine by the infected epithelium was significantly inhibited by pharmacological blockade of arginase.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions:<\/jats:title><jats:p>Intracellular catabolism by arginase II, the induction of which has not been described previously for intestinal epithelium, facilitates uptake of L\u2010arginine by infected epithelium using transport systems that do not differ from those of uninfected cells. Induction of arginase II may limit nitric oxide synthesis by competing with nitric oxide synthase for utilization of L\u2010arginine or promote use of L\u2010arginine for the synthesis of reparative polyamines.<\/jats:p><\/jats:sec>","DOI":"10.1097\/mpg.0b013e31816f6c02","type":"journal-article","created":{"date-parts":[[2008,10,10]],"date-time":"2008-10-10T07:09:00Z","timestamp":1223622540000},"page":"417-427","source":"Crossref","is-referenced-by-count":3,"title":["Induction of Arginase II by Intestinal Epithelium Promotes the Uptake of L\u2010Arginine From the Lumen of<i>Cryptosporidium parvum<\/i>\u2013infected Porcine Ileum"],"prefix":"10.1002","volume":"47","author":[{"given":"Jody L","family":"Gookin","sequence":"first","affiliation":[{"name":"Department of Clinical Sciences College of Veterinary Medicine North Carolina State University Raleigh USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephen 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