{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,28]],"date-time":"2025-11-28T04:46:08Z","timestamp":1764305168671},"reference-count":30,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2009,7,1]],"date-time":"2009-07-01T00:00:00Z","timestamp":1246406400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. pediatr. gastroenterol. nutr."],"published-print":{"date-parts":[[2009,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Objectives:<\/jats:title><jats:p>Assays of tissue transglutaminase antibodies (anti\u2010tTG) represent the cornerstone of serological coeliac disease (CD) diagnostics. Assays of antibodies against native gliadin (anti\u2010nGli) lost importance due to low validity. We investigated the performance of new assays for antibodies against deamidated gliadin (anti\u2010dGli) in childhood CD.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods:<\/jats:title><jats:p>We retrospectively compared children (142 with active CD and 160 without CD, diagnosis confirmed or excluded by intestinal biopsy) concerning (immunoglobulin [Ig] G and IgA) anti\u2010nGli, anti\u2010tTG, and 2 different anti\u2010dGli assays.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results:<\/jats:title><jats:p>IgG\u2010anti\u2010dGli1, IgG\u2010anti\u2010dGli2, and IgA\u2010anti\u2010tTG performed similarly. Area under the receiver\u2010operating characteristic curve (AUC) was 98.6%, 98.9%, and 97.9%; accuracy was 94.7%, 95.7%, and 96.7%. Anti\u2010dGli1 and anti\u2010dGli2 (IgG and IgA) and IgA\u2010anti\u2010tTG performed significantly better than IgA\u2010anti\u2010nGli and IgG\u2010anti\u2010nGli. Both IgG\u2010anti\u2010dGli showed higher AUC and accuracy than IgA\u2010anti\u2010dGli and IgG\u2010anti\u2010tTG. Combined evaluation of IgA\u2010anti\u2010tTG with one of the IgG\u2010anti\u2010dGli tests reduced the rate of falsely classified patients. At enhanced cutoff (specificity &gt;99%), sensitivity was above 67% for both IgG\u2010anti\u2010dGli and IgA\u2010anti\u2010tTG. If IgA\u2010anti\u2010tTG assay was combined with one of the IgG\u2010anti\u2010dGli tests, then the fraction of patients identified with more than 99% specificity as coeliacs increased significantly above 84.5%. Combined evaluation of the 2 IgG\u2010anti\u2010dGli tests did not improve the performance.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions:<\/jats:title><jats:p>The new IgA and IgG\u2010anti\u2010dGli tests outperform conventional anti\u2010nGli assays. The validity of IgG\u2010anti\u2010dGli cannot be distinguished from IgA\u2010anti\u2010tTG. It should be studied prospectively whether antibody assays could replace biopsy in diagnosis of CD in a substantial segment of children.<\/jats:p><\/jats:sec>","DOI":"10.1097\/mpg.0b013e318195dae3","type":"journal-article","created":{"date-parts":[[2009,6,22]],"date-time":"2009-06-22T05:08:48Z","timestamp":1245647328000},"page":"52-58","source":"Crossref","is-referenced-by-count":54,"title":["Antibodies Against Deamidated Gliadin as New and Accurate Biomarkers of Childhood Coeliac Disease"],"prefix":"10.1002","volume":"49","author":[{"given":"Christian","family":"Prause","sequence":"first","affiliation":[{"name":"Institute of Laboratory Medicine, Clinical Chemistry, and Molecular Diagnostics University Hospital Leipzig Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria","family":"Ritter","sequence":"additional","affiliation":[{"name":"Institute of Laboratory Medicine, Clinical Chemistry, and Molecular Diagnostics University Hospital Leipzig Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christian","family":"Probst","sequence":"additional","affiliation":[{"name":"EUROIMMUN Medizinische Labordiagnostika AG Luebeck Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cornelia","family":"Daehnrich","sequence":"additional","affiliation":[{"name":"EUROIMMUN Medizinische Labordiagnostika AG Luebeck Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wolfgang","family":"Schlumberger","sequence":"additional","affiliation":[{"name":"EUROIMMUN Medizinische Labordiagnostika AG Luebeck Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lars","family":"Komorowski","sequence":"additional","affiliation":[{"name":"EUROIMMUN Medizinische Labordiagnostika AG Luebeck Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ruediger","family":"Lieske","sequence":"additional","affiliation":[{"name":"A. Menarini Diagnostics Division Berlin\u2010Chemie AG Berlin Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas","family":"Richter","sequence":"additional","affiliation":[{"name":"Municipal Children's Hospital \u201cSankt Georg\u201d Leipzig Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Almuthe C","family":"Hauer","sequence":"additional","affiliation":[{"name":"Children's University Hospital Graz Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martin","family":"Stern","sequence":"additional","affiliation":[{"name":"Children's University Hospital Tuebingen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Holm H","family":"Uhlig","sequence":"additional","affiliation":[{"name":"Children's University Hospital Leipzig Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martin W","family":"Laass","sequence":"additional","affiliation":[{"name":"Children's University Hospital Dresden Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Klaus\u2010Peter","family":"Zimmer","sequence":"additional","affiliation":[{"name":"Children's University Hospital Giessen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas","family":"Mothes","sequence":"additional","affiliation":[{"name":"Institute of Laboratory Medicine, Clinical Chemistry, and Molecular Diagnostics University Hospital Leipzig Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,7]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra071600"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0016-5085(92)91819-P"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1097\/00042737-200501000-00017"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm0797-797"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1097\/00042737-200211000-00009"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2005.02.012"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2005.02.028"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2004.035832"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2249.2000.01312.x"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/47.11.2023"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2004.036111"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-2423(07)44002-1"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.13.1.150-151.2006"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cgh.2006.05.004"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cgh.2007.05.024"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2249.2007.03487.x"},{"key":"e_1_2_6_18_1","first-page":"1","article-title":"Resurrection of gliadin antibodies in coeliac disease. 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