{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,2,7]],"date-time":"2024-02-07T09:40:38Z","timestamp":1707298838532},"reference-count":25,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2010,2,1]],"date-time":"2010-02-01T00:00:00Z","timestamp":1264982400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. pediatr. gastroenterol. nutr."],"published-print":{"date-parts":[[2010,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Objectives:<\/jats:title><jats:p>Enterocyte apoptosis induced by activated intraepithelial lymphocytes is increased in coeliac disease (CD). Serum\u2010 and glucocorticoid\u2010regulated kinase\u20101 (SGK1) is a serine\/threonine protein kinase that may inhibit apoptosis and compensate for the excessive death of surface epithelial cells. The significance of SGK1 in CD is elusive so far. The aim of this study was to characterise the expression and localisation of SGK1 in duodenal biopsy samples taken from children with untreated CD, children with treated CD, and controls.<\/jats:p><\/jats:sec><jats:sec><jats:title>Patients and Methods:<\/jats:title><jats:p>Duodenal biopsy specimens were collected from 16 children with untreated CD, 9 children with treated CD, and 10 controls. The mRNA expression of SGK1 was determined by real\u2010time reverse transcription\u2010polymerase chain reaction. SGK1 and phosphorylated (P)\u2010SGK1 protein levels and their localisation were determined by Western blot and immunfluorescent staining, respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results:<\/jats:title><jats:p>We found increased SGK1\u2010mRNA expression as well as higher SGK1 and P\u2010SGK1 protein levels in the duodenal mucosa of children with untreated CD compared with controls. In the duodenal mucosa of children with treated CD, SGK1\u2010mRNA expression was decreased and SGK1 and P\u2010SGK1 protein levels were lower than in untreated CD. SGK1 and P\u2010SGK1 staining intensity was stronger in duodenal villous enterocytes of children with untreated CD compared with treated CD.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions:<\/jats:title><jats:p>Our results of increased expression of SGK1 in untreated CD may suggest its contribution to the enterocyte survival in this disease.<\/jats:p><\/jats:sec>","DOI":"10.1097\/mpg.0b013e3181b47608","type":"journal-article","created":{"date-parts":[[2010,1,16]],"date-time":"2010-01-16T07:55:16Z","timestamp":1263628516000},"page":"147-153","source":"Crossref","is-referenced-by-count":7,"title":["Increased Expression of Serum\u2010 and Glucocorticoid\u2010regulated Kinase\u20101 in the Duodenal Mucosa of Children With Coeliac Disease"],"prefix":"10.1002","volume":"50","author":[{"given":"Be\u00e1ta","family":"Szebeni","sequence":"first","affiliation":[{"name":"From the Research Group for Pediatrics and Nephrology Semmelweis University and Hungarian Academy of Sciences Budapest 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