{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,19]],"date-time":"2025-12-19T21:37:58Z","timestamp":1766180278840},"reference-count":29,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2010,2,1]],"date-time":"2010-02-01T00:00:00Z","timestamp":1264982400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["CA82312"],"award-info":[{"award-number":["CA82312"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007188","name":"Uniformed Services University of the Health Sciences","doi-asserted-by":"publisher","award":["CO86EN\u201001"],"award-info":[{"award-number":["CO86EN\u201001"]}],"id":[{"id":"10.13039\/100007188","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. pediatr. gastroenterol. nutr."],"published-print":{"date-parts":[[2010,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Background and Aim:<\/jats:title><jats:p>Gastroduodenal disease is more common among adults and children with <jats:italic>cagA<\/jats:italic>+ <jats:italic>Helicobacter pylori<\/jats:italic> infection, but disease severity varies among those infected with <jats:italic>cagA<\/jats:italic>+ strains. We examined whether <jats:italic>cagA<\/jats:italic> in situ expression can predict disease manifestations among <jats:italic>H pylori<\/jats:italic>\u2013infected children.<\/jats:p><\/jats:sec><jats:sec><jats:title>Patients and Methods:<\/jats:title><jats:p>Fifty\u2010one children were selected from 805 patients with abdominal symptoms who underwent esophagogastroduodenoscopy with gastric biopsies. Endoscopic and histologic gastritis were scored and <jats:italic>H pylori<\/jats:italic> colonization was quantified by Genta stain and in situ hybridization expression of <jats:italic>16S rRNA<\/jats:italic> and <jats:italic>cagA<\/jats:italic>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results:<\/jats:title><jats:p>Endoscopy was either normal (n = 14) or demonstrated nodularity (n = 18), gastric ulcer (n = 8) or duodenal ulcer (n = 11). <jats:italic>H pylori<\/jats:italic> was present in 7, 18, 6, and 10 children, respectively. Expression of <jats:italic>16S rRNA<\/jats:italic> and <jats:italic>cagA<\/jats:italic> were significantly higher in children with ulcer compared with normal children. The fraction of <jats:italic>H pylori<\/jats:italic> bacteria expressing <jats:italic>cagA<\/jats:italic> in situ was higher in children with ulcer compared to those with endoscopic nodularity (<jats:italic>P<\/jats:italic> &lt; 0.05).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions:<\/jats:title><jats:p>Thus, <jats:italic>cagA<\/jats:italic> in situ expression is increased in <jats:italic>H pylori<\/jats:italic>\u2013infected children with peptic ulcers and may play a role in the pathogenesis of peptic ulcer disease during childhood. Determination of in situ expression of <jats:italic>cagA<\/jats:italic> complements traditional isolation and in vitro testing of single\u2010colony isolates.<\/jats:p><\/jats:sec>","DOI":"10.1097\/mpg.0b013e3181bab326","type":"journal-article","created":{"date-parts":[[2010,1,16]],"date-time":"2010-01-16T07:55:16Z","timestamp":1263628516000},"page":"167-172","source":"Crossref","is-referenced-by-count":17,"title":["In Situ Expression of <i>cagA<\/i> and Risk of Gastroduodenal Disease in <i>Helicobacter pylori<\/i>\u2013infected Children"],"prefix":"10.1002","volume":"50","author":[{"given":"James R","family":"Rick","sequence":"first","affiliation":[{"name":"Department of Pediatrics USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matthew","family":"Goldman","sequence":"additional","affiliation":[{"name":"Department of Pediatrics USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cristina","family":"Semino\u2010Mora","sequence":"additional","affiliation":[{"name":"Department of Medicine USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hui","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Medicine USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cara","family":"Olsen","sequence":"additional","affiliation":[{"name":"Department of Preventive Medicine and Biometrics Uniformed Services University of the Health Sciences Bethesda MD USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eugenia","family":"Rueda\u2010Pedraza","sequence":"additional","affiliation":[{"name":"Department of Pathology USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carolyn","family":"Sullivan","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Walter Reed Army Medical Center Washington DC USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andre","family":"Dubois","sequence":"additional","affiliation":[{"name":"Department of Medicine USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2010,2]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.pathol.1.110304.100125"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1536-4801.2002.tb07791.x"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440-1746.1994.tb01294.x"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/174.3.552"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1136\/gut.45.6.804"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2006.12.012"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1349-7006.2005.00130.x"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1053\/gast.1996.v110.pm8964399"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.52.9.658"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1998.00770.x"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/aje\/155.11.1054"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.33.12.3300-3303.1995"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/CDLI.7.5.842-844.2000"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1086\/315262"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.10.3534-3537.2000"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.39.4.1348-1352.2001"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00535-004-1381-2"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/0046-8177(94)90191-0"},{"key":"e_1_2_5_20_1","doi-asserted-by":"crossref","unstructured":"LiuH RahmanA Semino\u2010MoraC DoiSQ DuboisA. 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