{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T20:35:45Z","timestamp":1777581345313,"version":"3.51.4"},"reference-count":31,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2012,3,1]],"date-time":"2012-03-01T00:00:00Z","timestamp":1330560000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000194","name":"Nova Scotia Health Research Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000194","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. pediatr. gastroenterol. nutr."],"published-print":{"date-parts":[[2012,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Objectives:<\/jats:title><jats:p>Polymorphonuclear leukocytes (PMN) feature prominently in the mucosa, including in crypt abscesses, of patients with inflammatory bowel disease, yet the mediators that are responsible for this migration are unknown. We discovered that CXCR2 chemokines (reportedly elevated in the mucosa) have reduced potency recruiting PMN across epithelial cell monolayers versus acellular filters, so the objective was to determine what molecules modify transepithelial PMN migration to CXCR2 chemokines.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods:<\/jats:title><jats:p>Transwells with T84 colon carcinoma monolayers or no epithelium were used with adolescent patient peripheral blood PMN and CXCL8 (interleukin\u20108 [IL\u20108], binds CXCR1 and CXCR2), CXCL5 (epithelial\u2010derived neutrophil chemoattractant\u201078 [ENA\u201078]), or CXCL1 (Gro\u2010\u03b1, both bind CXCR2) as chemoattractants.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results:<\/jats:title><jats:p>IL\u20108 was equally potent at recruiting PMN across filters and T84 monolayers growing on the filters. In contrast, ENA\u201078 and Gro\u2010\u03b1 were significantly less potent at recruiting PMN across monolayers than across bare filters. Blocking CXCR1 reduced PMN migration across monolayers to IL\u20108. We ruled out superoxide radicals possibly enhancing migration to IL\u20108 by using PMN from a patient with chronic granulomatous disease. PMN constitutively produce adenosine, so we added adenosine deaminase to the transwell assays and observed increased migration to ENA\u201078 across T84 monolayers. The level of migration was further enhanced by pretreating PMN with adenosine before adding the cells to the assay in the presence of the deaminase.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions:<\/jats:title><jats:p>PMN migration mediated by CXCR2 through the epithelium is regulated by adenosine. Adenosine appears to reduce transepithelial migration by influencing \u03b2<jats:sub>2<\/jats:sub>integrin use on the PMN.<\/jats:p><\/jats:sec>","DOI":"10.1097\/mpg.0b013e3182318d13","type":"journal-article","created":{"date-parts":[[2011,10,4]],"date-time":"2011-10-04T20:58:48Z","timestamp":1317761928000},"page":"414-421","source":"Crossref","is-referenced-by-count":6,"title":["Neutrophil Transintestinal Epithelial Migration to CXCR2 Ligands Is Regulated by Adenosine"],"prefix":"10.1002","volume":"54","author":[{"given":"Andrew W.","family":"Stadnyk","sequence":"first","affiliation":[{"name":"Department of Pediatrics Dalhousie University Dalhousie Inflammation Group and the IWK Health Centre Halifax Nova Scotia Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Svetlana O.","family":"Carrigan","sequence":"additional","affiliation":[{"name":"Department of Microbiology &amp; Immunology Dalhousie University Dalhousie Inflammation Group and the IWK Health Centre Halifax Nova Scotia Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anthony R.","family":"Otley","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Dalhousie University Dalhousie Inflammation Group and the IWK Health Centre Halifax Nova Scotia Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2012,3]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1358\/dot.2001.37.2.614846"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1572-0241.2003.07458.x"},{"key":"e_1_2_9_4_1","first-page":"2315","article-title":"Neutrophil\u2010elastase in chronic inflammatory bowel disease: a marker of disease activity","volume":"46","author":"Gouni\u2010Berthold I","year":"1999","journal-title":"Hepatogastroenterology"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.MPG.0000154657.08994.94"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI116462"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1155\/2002\/941087"},{"key":"e_1_2_9_8_1","first-page":"647","article-title":"Circulating growth\u2010regulated oncogene \u03b1 contributes to neutrophil priming and interleukin\u20108\u2010directed mucosal recruitment into chronic lesions of patients with Crohn's disease","volume":"9","author":"Brandt E","year":"1998","journal-title":"Eur Cytokine Netw"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0016-5085(97)70175-6"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1080\/003655201750313397"},{"key":"e_1_2_9_11_1","first-page":"G75","article-title":"Enterocytes are the primary source of the chemokine ENA\u201078 in normal colon and ulcerative colitis","volume":"273","author":"Keates S","year":"1997","journal-title":"Am J Physiol"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1572-0241.1999.00940.x"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/0016-5085(86)90684-0"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/1096-9896(2000)9999:9999<::AID-PATH732>3.0.CO;2-X"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2249.2004.02429.x"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/0016-5085(89)91410-8"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.0208125"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.0806528"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1432-1033.2003.03760.x"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi981294s"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.it.2003.11.003"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.200324807"},{"key":"e_1_2_9_23_1","first-page":"531","article-title":"The selective and superoxide\u2010independent disruption of intestinal epithelial tight junctions during leukocyte transmigration","volume":"59","author":"Nash S","year":"1988","journal-title":"Lab Invest"},{"key":"e_1_2_9_24_1","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1016\/S0022-3565(24)29668-3","article-title":"Adenosine kinase inhibitor GP515 improves experimental colitis in mice","volume":"296","author":"Siegmund B","year":"2001","journal-title":"J Pharmacol Exp Ther"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2005.05.032"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-2952(03)00067-4"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.05-4804fje"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1165\/ajrcmb\/9.2.179"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/jlb.59.5.671"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V95.2.519"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.5.2387"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI11783"}],"container-title":["Journal of Pediatric Gastroenterology and Nutrition"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1097\/MPG.0b013e3182318d13","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,3,12]],"date-time":"2025-03-12T12:44:06Z","timestamp":1741783446000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1097\/MPG.0b013e3182318d13"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,3]]},"references-count":31,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2012,3]]}},"alternative-id":["10.1097\/MPG.0b013e3182318d13"],"URL":"https:\/\/doi.org\/10.1097\/mpg.0b013e3182318d13","archive":["Portico"],"relation":{},"ISSN":["0277-2116","1536-4801"],"issn-type":[{"value":"0277-2116","type":"print"},{"value":"1536-4801","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,3]]}}}