{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T13:38:56Z","timestamp":1782826736507,"version":"3.54.5"},"reference-count":16,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2012,7,1]],"date-time":"2012-07-01T00:00:00Z","timestamp":1341100800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. pediatr. gastroenterol. nutr."],"published-print":{"date-parts":[[2012,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Objective:<\/jats:title><jats:p>Assays for antibodies against native gliadin (anti\u2010nGli) are still often assumed to perform better in the diagnosis of coeliac disease in young children than tests for antibodies to deamidated gliadin (anti\u2010dGli), tissue transglutaminase (anti\u2010tTG), and endomysium (EmA). We compared the performance of assays for anti\u2010nGli, anti\u2010dGli, anti\u2010tTG, and EmA in this age group.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods:<\/jats:title><jats:p>We investigated retrospectively 184 children (42 with coeliac disease under normal diet and 142 controls) up to 2 years of age. Immunoglobulin (Ig) A\u2010 and IgG\u2010anti\u2010dGli, IgA\u2010 and IgG\u2010anti\u2010nGli, IgA\u2010 and IgG\u2010anti\u2010tTG, and IgA\u2010EmA were measured in serum. Areas under receiver operating characteristics curves, sensitivities, specificities, positive and negative predictive values, positive and negative likelihood ratios, as well as diagnostic odds ratios were calculated.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results:<\/jats:title><jats:p>From all of the tests investigated, only assays for IgG\u2010anti\u2010dGli, IgA\u2010anti\u2010tTG, and IgA\u2010EmA had high specificity (\u22650.96) connected with high sensitivity (\u22650.86), with high positive predictive values (\u22650.52 and \u22650.69 at pretest probabilities of 0.05 and 0.1, respectively) and negative predictive values (\u22650.99 and \u22650.98 at pretest probabilities of 0.05 and 0.1, respectively). These assays also showed high positive likelihood ratio (\u226524) at low negative likelihood ratio (\u22640.15) and high diagnostic odds ratios (\u2265136).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions:<\/jats:title><jats:p>Our results do not support the use of assays of anti\u2010nGli to diagnose coeliac disease in young children. IgA\u2010anti\u2010tTG, IgA\u2010EmA, and IgG\u2010anti\u2010dGli perform better than anti\u2010nGli.<\/jats:p><\/jats:sec>","DOI":"10.1097\/mpg.0b013e31824678fc","type":"journal-article","created":{"date-parts":[[2012,1,14]],"date-time":"2012-01-14T20:26:20Z","timestamp":1326572780000},"page":"21-25","source":"Crossref","is-referenced-by-count":10,"title":["Determination of IgG and IgA Antibodies Against Native Gliadin Is Not Helpful for the Diagnosis of Coeliac Disease in Children Up to 2 Years Old"],"prefix":"10.1002","volume":"55","author":[{"given":"Thomas","family":"Richter","sequence":"first","affiliation":[{"name":"Children's Hospital of the Clinical Centre \u201cSankt Georg\u201d Leipzig Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xavier","family":"Bossuyt","sequence":"additional","affiliation":[{"name":"Department Laboratory Medicine of University Hospital Leuven Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pieter","family":"Vermeersch","sequence":"additional","affiliation":[{"name":"Department Laboratory Medicine of University Hospital Leuven Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Holm H.","family":"Uhlig","sequence":"additional","affiliation":[{"name":"University Children's Hospital Leipzig Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Stern","sequence":"additional","affiliation":[{"name":"University Children's Hospital T\u00fcbingen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Almuthe","family":"Hauer","sequence":"additional","affiliation":[{"name":"University Children's Hospital Graz Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Klaus\u2010Peter","family":"Zimmer","sequence":"additional","affiliation":[{"name":"University Children's Hospital Giessen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luisa","family":"Mearin","sequence":"additional","affiliation":[{"name":"Department of Paediatrics Leiden University Medical Centre Leiden The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johanna Hendrika Clementina","family":"de Roo","sequence":"additional","affiliation":[{"name":"Department of Paediatrics Leiden University Medical Centre Leiden The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cornelia","family":"D\u00e4hnrich","sequence":"additional","affiliation":[{"name":"EUROIMMUN Medizinische Labordiagnostika GmbH L\u00fcbeck Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Mothes","sequence":"additional","affiliation":[{"name":"Institute of Laboratory Medicine Clinical Chemistry and Molecular Diagnostics University Hospital Leipzig 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