{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,6,3]],"date-time":"2024-06-03T22:36:24Z","timestamp":1717454184190},"reference-count":38,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2013,6,1]],"date-time":"2013-06-01T00:00:00Z","timestamp":1370044800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. pediatr. gastroenterol. nutr."],"published-print":{"date-parts":[[2013,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Objective:<\/jats:title><jats:p>Preterm infants often show intolerance to the first enteral feeds, and the structural and functional basis of this intolerance remains unclear. We hypothesized that preterm and term neonates show similar gut trophic responses to feeding but different expression of intestinal functional proteins, thus helping to explain why preterm neonates are more susceptible to feeding\u2010induced disorders such as necrotizing enterocolitis (NEC).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods:<\/jats:title><jats:p>Incidence of feeding\u2010induced NEC, intestinal mass, and brush border enzyme activities, and the intestinal proteome in preterm cesarean\u2010delivered pigs were compared with the corresponding values in pigs delivered spontaneously at term.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results:<\/jats:title><jats:p>For both preterm and term pigs, mucosal mass and maltase activity increased (50%\u2013100%), whereas lactase decreased (\u221250%), relative to values at birth. Only preterm pigs were highly NEC sensitive (30% vs 0% in term pigs, <jats:italic>P<\/jats:italic> &lt; 0.05). By gel\u2010based proteomics, 36 identified proteins differed in expression, with most proteins showing downregulation in preterm pigs, including proteins related to intestinal structure and actin filaments, stress response, protein processing, and nutrient metabolism.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions:<\/jats:title><jats:p>Despite that enteral feeding induces rapid gut tropic response in both term and preterm neonates, the expression level of cellular proteins related to mucosal integrity, metabolism, and stress response differed markedly (including complement 3, prohibitin, ornithine carbamoyltransferase, and arginosuccinate synthetase). These proteins may play a role in the development of functional gut disorders and NEC in preterm neonates.<\/jats:p><\/jats:sec>","DOI":"10.1097\/mpg.0b013e318288cf71","type":"journal-article","created":{"date-parts":[[2013,1,30]],"date-time":"2013-01-30T09:15:06Z","timestamp":1359537306000},"page":"615-622","source":"Crossref","is-referenced-by-count":11,"title":["Premature Delivery Reduces Intestinal Cytoskeleton, Metabolism, and Stress Response Proteins in Newborn Formula\u2010Fed Pigs"],"prefix":"10.1002","volume":"56","author":[{"given":"Pingping","family":"Jiang","sequence":"first","affiliation":[{"name":"School of Biological Sciences University of Hong Kong Hong Kong SAR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jennifer Man\u2010Fan","family":"Wan","sequence":"additional","affiliation":[{"name":"School of Biological Sciences University of Hong Kong Hong Kong SAR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Malene S.","family":"Cilieborg","sequence":"additional","affiliation":[{"name":"Department of Human Nutrition Faculty of Science University of Copenhagen Frederiksberg Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wai\u2010Hung","family":"Sit","sequence":"additional","affiliation":[{"name":"School of Biological Sciences University of Hong Kong Hong Kong SAR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Per Torp","family":"Sangild","sequence":"additional","affiliation":[{"name":"Department of Human Nutrition Faculty of Science University of Copenhagen Frederiksberg 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