{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,4]],"date-time":"2025-05-04T05:47:40Z","timestamp":1746337660671},"reference-count":27,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2013,12,1]],"date-time":"2013-12-01T00:00:00Z","timestamp":1385856000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["30973139"],"award-info":[{"award-number":["30973139"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. pediatr. gastroenterol. nutr."],"published-print":{"date-parts":[[2013,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Objectives:<\/jats:title><jats:p>Biliary atresia (BA) is a devastating pediatric cholestatic liver disease. Increasing evidence indicates that nuclear factor (NF)\u2010\u03baB signaling plays a key role in the pathogenesis of BA. Leucine zipper downregulated in cancer 1 (LDOC1) may control the expression of NF\u2010\u03baB. The aim of this study was to evaluate the relation between LDOC1 and inflammation\/apoptosis mediated by NF\u2010\u03baB in the human intrahepatic biliary epithelial cells (HIBECs).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods:<\/jats:title><jats:p>HIBECs were divided into 3 treatment groups: control, mock transfection group, and LDOC1 transfection. Immunofluorescence, reverse transcription polymerase chain reaction, Western blot, and flow cytometry analysis were used to investigate the effectiveness of LDOC1\u2010transfected HIBECs and the expression of NF\u2010\u03baB. Apoptosis was detected by Hochest\/ propidium iodide staining. Interleukin (IL)\u20102 and tumor necrosis factor (TNF)\u2010\u03b1 levels were evaluated by enzyme\u2010linked immunosorbent assay.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results:<\/jats:title><jats:p>The expression of NF\u2010\u03baB was higher in the LDOC1\u2010transfected group when compared with the control and mock\u2010transfected groups as evaluated by immunofluorescence, reverese transcription polymerase chain reaction, and Western blot analysis. The rate of apoptosis was significantly lower in the LDOC1\u2010transfected group when compared with the control and mock\u2010transfected groups. The levels of IL\u20102 and TNF\u2010\u03b1 were significantly higher in the LDOC1\u2010transfected group when compared with the control and mock\u2010transfected groups.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions:<\/jats:title><jats:p>Upregulation of LDOC1 in HIBEC increases the expression of NF\u2010\u03baB, which may promote the activation of IL\u20102 and TNF\u2010\u03b1 secretion and inhibit cell apoptosis.<\/jats:p><\/jats:sec>","DOI":"10.1097\/mpg.0b013e3182a7e1da","type":"journal-article","created":{"date-parts":[[2013,8,13]],"date-time":"2013-08-13T11:48:35Z","timestamp":1376394515000},"page":"713-717","source":"Crossref","is-referenced-by-count":5,"title":["Overexpression of LDOC1 in Human Biliary Epithelial Cells Inhibits Apoptosis Through NF\u2010\u03baB Signaling"],"prefix":"10.1002","volume":"57","author":[{"given":"Zai","family":"Song","sequence":"first","affiliation":[{"name":"Department of Pediatric Surgery Children's Hospital of Fudan University Key Laboratory of Neonatal Disease Ministry of Health Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rui","family":"Dong","sequence":"additional","affiliation":[{"name":"Department of Pediatric Surgery Children's Hospital of Fudan University Key Laboratory of Neonatal Disease Ministry of Health Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rui","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Pediatric Surgery Children's Hospital of Fudan University Key Laboratory of Neonatal Disease Ministry of Health Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shan","family":"Zheng","sequence":"additional","affiliation":[{"name":"Department of Pediatric Surgery Children's Hospital of Fudan University Key Laboratory of Neonatal Disease Ministry of Health Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,12]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.sempedsurg.2012.05.010"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.mpg.0000189339.92891.64"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpedsurg.2004.02.020"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-3046.2010.01340.x"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(02)11603-5"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-3046.2005.00350.x"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12016-012-8324-0"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(09)60946-6"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2010-09-304881"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-3835(99)00087-7"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.11122"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1209954"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm2083"},{"key":"e_1_2_9_15_1","first-page":"343","article-title":"NF\u2010kappaB and its regulation on the immune system","volume":"1","author":"Liang Y","year":"2004","journal-title":"Cell Mol Immunol"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri910"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI11830"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri957"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri2214"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaut.2007.06.003"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaut.2007.04.002"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaut.2006.11.004"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.21968"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI57728"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpedsurg.2004.12.004"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.2741\/A171"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1442-200X.2008.02561.x"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00467-004-1680-0"}],"container-title":["Journal of Pediatric Gastroenterology and Nutrition"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1097\/MPG.0b013e3182a7e1da","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,18]],"date-time":"2024-01-18T13:12:39Z","timestamp":1705583559000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1097\/MPG.0b013e3182a7e1da"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,12]]},"references-count":27,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2013,12]]}},"alternative-id":["10.1097\/MPG.0b013e3182a7e1da"],"URL":"https:\/\/doi.org\/10.1097\/mpg.0b013e3182a7e1da","archive":["Portico"],"relation":{},"ISSN":["0277-2116","1536-4801"],"issn-type":[{"value":"0277-2116","type":"print"},{"value":"1536-4801","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,12]]}}}