{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,9]],"date-time":"2026-04-09T13:51:20Z","timestamp":1775742680392,"version":"3.50.1"},"reference-count":38,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2011,1,1]],"date-time":"2011-01-01T00:00:00Z","timestamp":1293840000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2011,1,1]],"date-time":"2011-01-01T00:00:00Z","timestamp":1293840000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100002476","name":"Eulji University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002476","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["OVS"],"published-print":{"date-parts":[[2011,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Purpose.<\/jats:title>\n                    <jats:p>The purpose of this study was to identify novel autoantigens that react with specific serum autoantibodies in patients with glaucoma.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods.<\/jats:title>\n                    <jats:p>Sera from patients with glaucoma (n = 80) and healthy subjects without a known pathology (n = 20) were investigated by immunoblot performed with bovine optic nerve lysates and resolved by one\u2010 and two\u2010dimensional electrophoresis. Proteins in the immunoreactive spots were selected and identified by liquid chromatography\u2010tandem mass spectrometry (LC\u2010MS\/MS) data analysis. All the sera from subjects were assayed using enzyme\u2010linked immunosorbent assay to identify autoantibodies.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results.<\/jats:title>\n                    <jats:p>We selected two prominent bands with molecular weights of 100 and 220 kDa by 8% sodium dodecyl sulfate\u2010polyacrylamide gel electrophoresis analysis, and these two bands were only found in the glaucoma patients. Using one\u2010dimensional electrophoresis and LC\u2010MS\/MS analyses, we identified these proteins to be valosin\u2010containing protein (VCP) and fodrin, respectively, and using two\u2010dimensional electrophoresis and LC\u2010MS\/MS analyses, VCP was identified to be a common target antigen. In patients with primary open angle glaucoma and normal tension glaucoma, the frequency of autoantibodies to recombinant human VCP was 42.0 and 23.3%, respectively (p &lt; 0.002). In the enzyme\u2010linked immunosorbent assay tests, autoantibody titers to recombinant human VCP were significantly higher than that in healthy controls (p &lt; 0.025).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions.<\/jats:title>\n                    <jats:p>VCP represents a potential candidate target for autoantibodies on the optic nerve in patients with glaucoma.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1097\/opx.0b013e3181fa6312","type":"journal-article","created":{"date-parts":[[2010,10,8]],"date-time":"2010-10-08T07:39:09Z","timestamp":1286523549000},"page":"164-172","source":"Crossref","is-referenced-by-count":1,"title":["Valosin\u2010Containing Protein is a Novel Autoantigen in Patients with Glaucoma"],"prefix":"10.1002","volume":"88","author":[{"given":"Koon\u2010Ja","family":"Lee","sequence":"first","affiliation":[{"name":"Department of Optometry Eulji University Sungnam Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seon\u2010Mi","family":"Jeong","sequence":"additional","affiliation":[{"name":"Division of Proteomics Department of Diagnosis Development R&amp;D Center Lumieye Genetics Co., Ltd. Seoul Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Benjamin Douglass","family":"Hoehn","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery University of Virginia Charlottesville Virginia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Young Jae","family":"Hong","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology Nune Eye Hospital Seoul Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sung Ho","family":"Lee","sequence":"additional","affiliation":[{"name":"Division of Proteomics Department of Diagnosis Development R&amp;D Center Lumieye Genetics Co., Ltd. Seoul Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2011,1,1]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1136\/bjo.80.5.389","article-title":"Number of people with glaucoma worldwide","volume":"80","author":"Quigley HA","year":"1996","journal-title":"Br J Ophthalmol"},{"key":"e_1_2_6_3_1","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/S0896-1549(01)00012-8","article-title":"Glaucomatous optic neuropathy: mechanisms of disease","volume":"15","author":"Halpern DL","year":"2002","journal-title":"Ophthalmol Clin North Am"},{"key":"e_1_2_6_4_1","doi-asserted-by":"crossref","first-page":"41","DOI":"10.3341\/kjo.1987.1.1.41","article-title":"Clinical study of Korean glaucomatous patients","volume":"1","author":"Hong C","year":"1987","journal-title":"Korean J 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Ophthalmol Vis Sci"},{"key":"e_1_2_6_12_1","first-page":"1968","article-title":"Anti\u2010rhodopsin antibodies in sera from patients with normal\u2010pressure glaucoma","volume":"36","author":"Romano C","year":"1995","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"e_1_2_6_13_1","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1016\/S0002-9394(99)80084-1","article-title":"Anti\u2010Ro\/SS\u2010A positivity and heat shock protein antibodies in patients with normal\u2010pressure glaucoma","volume":"125","author":"Wax MB","year":"1998","journal-title":"Am J Ophthalmol"},{"key":"e_1_2_6_14_1","first-page":"2277","article-title":"Autoantibodies to small heat shock proteins in glaucoma","volume":"39","author":"Tezel G","year":"1998","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"e_1_2_6_15_1","first-page":"1657","article-title":"Retinal ganglion cells recognized by serum autoantibody against gamma\u2010enolase found in glaucoma patients","volume":"41","author":"Maruyama 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IT","year":"1993","journal-title":"Nature"},{"key":"e_1_2_6_28_1","doi-asserted-by":"crossref","first-page":"3648","DOI":"10.1074\/jbc.M208422200","article-title":"ATPase activity of p97\u2010valosin\u2010containing protein (VCP). D2 mediates the major enzyme activity, and D1 contributes to the heat\u2010induced activity","volume":"278","author":"Song C","year":"2003","journal-title":"J Biol Chem"},{"key":"e_1_2_6_29_1","doi-asserted-by":"crossref","first-page":"855","DOI":"10.1083\/jcb.200208112","article-title":"VCIP135, a novel essential factor for p97\/p47\u2010mediated membrane fusion, is required for Golgi and ER assembly in vivo","volume":"159","author":"Uchiyama K","year":"2002","journal-title":"J Cell Biol"},{"key":"e_1_2_6_30_1","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1016\/j.bbamcr.2005.03.011","article-title":"Golgi reassembly after mitosis: the AAA family meets the ubiquitin family","volume":"1744","author":"Meyer HH","year":"2005","journal-title":"Biochim Biophys Acta"},{"key":"e_1_2_6_31_1","doi-asserted-by":"crossref","first-page":"7166","DOI":"10.1074\/jbc.M705893200","article-title":"A proteasomal ATPase contributes to dislocation of endoplasmic reticulum\u2010associated degradation (ERAD) substrates","volume":"283","author":"Lipson C","year":"2008","journal-title":"J Biol Chem"},{"key":"e_1_2_6_32_1","doi-asserted-by":"crossref","first-page":"740","DOI":"10.1038\/35087056","article-title":"Valosin\u2010containing protein is a multi\u2010ubiquitin chain\u2010targeting factor required in ubiquitin\u2010proteasome degradation","volume":"3","author":"Dai RM","year":"2001","journal-title":"Nat Cell Biol"},{"key":"e_1_2_6_33_1","doi-asserted-by":"crossref","first-page":"4221","DOI":"10.1091\/mbc.e03-02-0111","article-title":"DNA damage modulates nucleolar interaction of the Werner protein with the AAA ATPase p97\/VCP","volume":"14","author":"Partridge JJ","year":"2003","journal-title":"Mol Biol Cell"},{"key":"e_1_2_6_34_1","doi-asserted-by":"crossref","first-page":"3533","DOI":"10.1002\/j.1460-2075.1992.tb05436.x","article-title":"VCP, the mammalian homolog of cdc48, is tyrosine phosphorylated in response to T cell antigen receptor activation","volume":"11","author":"Egerton M","year":"1992","journal-title":"EMBO J"},{"key":"e_1_2_6_35_1","doi-asserted-by":"crossref","first-page":"396","DOI":"10.1016\/S0168-9525(98)01559-5","article-title":"Protein precipitation: a common etiology in neurodegenerative disorders?","volume":"14","author":"Kakizuka A","year":"1998","journal-title":"Trends Genet"},{"key":"e_1_2_6_36_1","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1016\/S0165-5728(97)00066-0","article-title":"Anti\u2010brain spectrin immunoreactivity in Alzheimer's disease: degradation of spectrin in an animal model of cholinergic degeneration","volume":"77","author":"Fern\u00e1ndez\u2010Shaw C","year":"1997","journal-title":"J Neuroimmunol"},{"key":"e_1_2_6_37_1","doi-asserted-by":"crossref","first-page":"604","DOI":"10.1126\/science.276.5312.604","article-title":"Identification of alpha\u2010fodrin as a candidate autoantigen in primary Sjogren's syndrome","volume":"276","author":"Haneji N","year":"1997","journal-title":"Science"},{"key":"e_1_2_6_38_1","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/S1568-9972(03)00002-8","article-title":"Antibodies against alpha\u2010fodrin in Sjogren's syndrome","volume":"2","author":"Ulbricht KU","year":"2003","journal-title":"Autoimmun Rev"},{"key":"e_1_2_6_39_1","doi-asserted-by":"crossref","first-page":"681","DOI":"10.1097\/00019052-199310000-00002","article-title":"Immune\u2010mediated neuropathies","volume":"6","author":"Kornberg AJ","year":"1993","journal-title":"Curr Opin Neurol"}],"container-title":["Optometry and Vision Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1097\/OPX.0b013e3181fa6312","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1097\/OPX.0b013e3181fa6312","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1097\/OPX.0b013e3181fa6312","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,31]],"date-time":"2026-03-31T09:38:17Z","timestamp":1774949897000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1097\/OPX.0b013e3181fa6312"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,1]]},"references-count":38,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2011,1]]}},"alternative-id":["10.1097\/OPX.0b013e3181fa6312"],"URL":"https:\/\/doi.org\/10.1097\/opx.0b013e3181fa6312","archive":["Portico"],"relation":{},"ISSN":["1040-5488","1538-9235"],"issn-type":[{"value":"1040-5488","type":"print"},{"value":"1538-9235","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,1]]}}}