{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,21]],"date-time":"2026-02-21T06:22:33Z","timestamp":1771654953105,"version":"3.50.1"},"reference-count":8,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"9","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,10]]},"abstract":"<jats:sec>\n            <jats:title>Background:<\/jats:title>\n            <jats:p>Metastatic melanoma in sentinel lymph nodes is often elusive to detect with morphology alone. Per American Joint Committee on Cancer staging guidelines, a single atypical melanocyte in lymph node qualifies as metastasis, whether identified by morphology or immunohistochemistry, but single cell staining must be convincing. We propose that the use of a second immunohistochemical run performed on a single slide will allow for more confident diagnosis of micrometastases.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Materials and Methods:<\/jats:title>\n            <jats:p>We designed a technical study to determine whether a second antibody application on previously stained slides can successfully detect the same population of cells. Melanocytic neoplasms were stained with SOX-10 using Ventana Benchmark Ultra stainers, coverslipped, and examined, followed by coverslip removal and application of MART-1 (Ventana A103). The order of antibody application and chromagen detection kit (AP-RED vs. DAB) was reversed to establish reliability and robustness of the protocol.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results:<\/jats:title>\n            <jats:p>All melanocytes marked with SOX-10 and MART-1, and produced a range of staining quality that varied based on order of stain application and chromagen kit were used. The optimal combination was red MART-1 applied first followed by brown SOX-10 applied second.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusions:<\/jats:title>\n            <jats:p>Consecutive staining of melanocytes with SOX-10 and MART-1 may improve diagnostic confidence of melanocyte identification, particularly in detection of single cell, micrometastases in sentinel lymph nodes or in situations where dual immunohistochemical stains may be unavailable.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1097\/pai.0000000000000665","type":"journal-article","created":{"date-parts":[[2018,11,29]],"date-time":"2018-11-29T13:38:00Z","timestamp":1543498680000},"page":"e76-e81","source":"Crossref","is-referenced-by-count":3,"title":["Consecutive Immunohistochemical Staining on a Single Slide is Effective in Confirming Melanocytic Derivation"],"prefix":"10.1097","volume":"28","author":[{"given":"Katelynn K.","family":"Campbell","sequence":"first","affiliation":[{"name":"Pathology"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jerad M.","family":"Gardner","sequence":"additional","affiliation":[{"name":"Pathology"},{"name":"Dermatology, University of Arkansas for Medical Sciences, Little Rock, AR"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kim","family":"Hall","sequence":"additional","affiliation":[{"name":"Pathology"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amanda","family":"Osborne","sequence":"additional","affiliation":[{"name":"Pathology"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sara C.","family":"Shalin","sequence":"additional","affiliation":[{"name":"Pathology"},{"name":"Dermatology, University of Arkansas for Medical Sciences, Little Rock, AR"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"276","published-online":{"date-parts":[[2018,11,28]]},"reference":[{"key":"R1-20230922","doi-asserted-by":"crossref","first-page":"1603","DOI":"10.5858\/arpa.2012-0550-OA","article-title":"Lack of standards for the detection of melanoma in sentinel lymph nodes: a survey and recommendations","volume":"137","author":"Dekker","year":"2013","journal-title":"Arch Pathol Lab Med"},{"key":"R2-20230922","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1002\/jso.21876","article-title":"Update on the melanoma staging system: the importance of sentinel node staging and primary tumor mitotic rate","volume":"104","author":"Balch","year":"2011","journal-title":"J Surg Oncol"},{"key":"R3-20230922","doi-asserted-by":"crossref","first-page":"2211","DOI":"10.1056\/NEJMoa1613210","article-title":"Completion dissection or observation for sentinel-node metastasis in melanoma","volume":"376","author":"Faries","year":"2017","journal-title":"N Engl J Med"},{"key":"R4-20230922","doi-asserted-by":"crossref","first-page":"2452","DOI":"10.1200\/JCO.2009.27.1627","article-title":"Multivariate analysis of prognostic factors among 2,313 patients with stage III melanoma: comparison of nodal micrometastases versus macrometastases","volume":"28","author":"Balch","year":"2010","journal-title":"J Clin Oncol"},{"key":"R5-20230922","doi-asserted-by":"crossref","first-page":"779","DOI":"10.1002\/cncy.21458","article-title":"Reliability of immunostaining using pan-melanoma cocktail, SOX-10, and microphthalmia transcription factor in confirming a diagnosis of melanoma on fine needle aspiration smears","volume":"122","author":"Clevenger","year":"2014","journal-title":"Cancer Cyopathol"},{"key":"R6-20230922","doi-asserted-by":"crossref","first-page":"196","DOI":"10.1080\/09674845.2002.11783659","article-title":"Evaluation of melanocytic neoplasms: application of a pan-melanoma antibody cocktail","volume":"59","author":"Orchard","year":"2002","journal-title":"Br J Biomed Sci"},{"key":"R7-20230922","first-page":"797","article-title":"Nests with numerous SOX-10 and MiTF-positive cells in lichenoid inflammation: authentic melanocytic proliferation?","volume":"38","author":"Silva","year":"2011","journal-title":"J Cutan Pathol"},{"key":"R9-20230922","doi-asserted-by":"crossref","first-page":"461","DOI":"10.1016\/j.oooo.2014.05.013","article-title":"Pseudomelanocytic nests mimicking atypical melanocytic proliferations: first reported cases in the oral cavity","volume":"118","author":"Boros","year":"2014","journal-title":"Oral Surg Oral Med Oral Pathol Oral Radiol"}],"container-title":["Applied Immunohistochemistry &amp; Molecular Morphology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.lww.com\/10.1097\/PAI.0000000000000665","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,22]],"date-time":"2023-09-22T19:00:21Z","timestamp":1695409221000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.lww.com\/10.1097\/PAI.0000000000000665"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,11,28]]},"references-count":8,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2020]]}},"URL":"https:\/\/doi.org\/10.1097\/pai.0000000000000665","relation":{},"ISSN":["1541-2016"],"issn-type":[{"value":"1541-2016","type":"print"}],"subject":[],"published":{"date-parts":[[2018,11,28]]}}}