{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,10]],"date-time":"2026-11-10T21:09:20Z","timestamp":1794344960628,"version":"4.9.0"},"reference-count":31,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"9","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2018,9]]},"abstract":"<jats:p>We describe a novel gene fusion, <jats:italic toggle=\"yes\">EWSR1-CREM<\/jats:italic>, identified in 3 cases of clear cell carcinoma (CCC) using anchored multiplex polymerase chain reaction, a next-generation sequencing\u2013based technique. CCC is a low-grade salivary tumor recently characterized to have <jats:italic toggle=\"yes\">EWSR1-ATF1<\/jats:italic> fusions in the majority of cases. Three cases of malignant tumor presenting in the base of tongue, lung, and nasopharynx were studied. All cases shared a clear cell morphology with hyalinized stroma, presence of mucin and p63 positivity and were initially diagnosed as mucoepidermoid carcinoma but were negative for evidence of any of the expected gene fusions. Anchored multiplex polymerase chain reaction demonstrated a <jats:italic toggle=\"yes\">EWSR1-CREM<\/jats:italic> fusion in all 3 cases to confirm a diagnosis of CCC. This finding is biologically justified as <jats:italic toggle=\"yes\">CREM<\/jats:italic> and <jats:italic toggle=\"yes\">ATF1<\/jats:italic> both belong to the CREB family of transcription factors. <jats:italic toggle=\"yes\">EWSR1-CREM<\/jats:italic> fusions have not been previously reported in CCC and have only rarely been reported in other tumors. We show that the ability to discover novel gene variants with next-generation sequencing\u2013based assays has clinical utility in the pathologic classification of fusion gene-associated tumors.<\/jats:p>","DOI":"10.1097\/pas.0000000000001114","type":"journal-article","created":{"date-parts":[[2018,7,3]],"date-time":"2018-07-03T23:14:22Z","timestamp":1530659662000},"page":"1182-1189","source":"Crossref","is-referenced-by-count":112,"title":["Molecular Profiling of Hyalinizing Clear Cell Carcinomas Revealed a Subset of Tumors Harboring a Novel EWSR1-CREM Fusion"],"prefix":"10.1097","volume":"42","author":[{"given":"Erin","family":"Chapman","sequence":"first","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, University of British Columbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alena","family":"Skalova","sequence":"additional","affiliation":[{"name":"Department of Pathology, Faculty of Medicine in Plzen, Charles University"},{"name":"CO Bioptic Laboratory Ltd"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nikola","family":"Ptakova","sequence":"additional","affiliation":[{"name":"Czech Republic Bioptic Laboratory, Ltd, Molecular Pathology Laboratory, Plzen, Czechia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Petr","family":"Martinek","sequence":"additional","affiliation":[{"name":"Czech Republic Bioptic Laboratory, Ltd, Molecular Pathology Laboratory, Plzen, Czechia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angela","family":"Goytain","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, University of British Columbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tracy","family":"Tucker","sequence":"additional","affiliation":[{"name":"B.C. 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