{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,17]],"date-time":"2026-06-17T13:52:51Z","timestamp":1781704371428,"version":"3.54.5"},"reference-count":42,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"7","license":[{"start":{"date-parts":[[2026,3,25]],"date-time":"2026-03-25T00:00:00Z","timestamp":1774396800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:p>\n                    Epithelial-myoepithelial carcinoma (EMC) is a rare malignant tumor of the salivary glands, often characterized by\n                    <jats:italic toggle=\"yes\">HRAS<\/jats:italic>\n                    mutations. The present study aimed to better define the molecular and transcriptomic landscape of EMC and explore its relationship with other head and neck neoplasms, particularly basal cell adenoma (BCA). We retrospectively analyzed 14 EMC cases using histology, immunohistochemistry, and whole-exome capture RNA sequencing.\n                    <jats:italic toggle=\"yes\">HRAS<\/jats:italic>\n                    mutations were identified in 57.0% (8\/14) of EMC, predominantly at p.Gly61Arg. Additional mutations identified included\n                    <jats:italic toggle=\"yes\">PIK3CA<\/jats:italic>\n                    ,\n                    <jats:italic toggle=\"yes\">STAT5B<\/jats:italic>\n                    , and\n                    <jats:italic toggle=\"yes\">NOTCH1<\/jats:italic>\n                    , as well as gene fusions such as\n                    <jats:italic toggle=\"yes\">HMGA2<\/jats:italic>\n                    ::\n                    <jats:italic toggle=\"yes\">WIF1<\/jats:italic>\n                    and\n                    <jats:italic toggle=\"yes\">FBXO32<\/jats:italic>\n                    ::\n                    <jats:italic toggle=\"yes\">PLAG1<\/jats:italic>\n                    . A comparative analysis with 54\n                    <jats:italic toggle=\"yes\">RAS<\/jats:italic>\n                    -mutated head and neck tumors revealed that\n                    <jats:italic toggle=\"yes\">HRAS<\/jats:italic>\n                    mutations were not exclusive to EMC, as they were also found in benign entities such as BCAs. Transcriptomic profiling found that EMC and BCA shared significant gene expression similarities, clustering closely. Differential expression analysis found upregulation of\n                    <jats:italic toggle=\"yes\">GATA3<\/jats:italic>\n                    ,\n                    <jats:italic toggle=\"yes\">CHI3L1<\/jats:italic>\n                    ,\n                    <jats:italic toggle=\"yes\">GRIA2<\/jats:italic>\n                    , and\n                    <jats:italic toggle=\"yes\">MME<\/jats:italic>\n                    in EMC, while BCAs had enrichment of Wnt-beta-catenin signaling. In contrast, EMCs had activation of the Ras-Raf-MAPK and PI3K-Akt pathways, supported by upregulation of E2F and G2-M checkpoint targets. Immunohistochemistry confirmed GATA3 positivity in\n                    <jats:italic toggle=\"yes\">HRAS<\/jats:italic>\n                    -mutated EMCs. The frequent\n                    <jats:italic toggle=\"yes\">RAS<\/jats:italic>\n                    mutations and overlapping transcriptomic profiles raise important diagnostic considerations and highlight the need for integrated molecular analyses to differentiate these entities. The EMCs displayed a recurrent\n                    <jats:italic toggle=\"yes\">HRAS<\/jats:italic>\n                    mutation, also observed in other head and neck tumors, and were partially clustered with the cases of BCA, suggesting potential biological similarities.\n                  <\/jats:p>","DOI":"10.1097\/pas.0000000000002541","type":"journal-article","created":{"date-parts":[[2026,3,25]],"date-time":"2026-03-25T17:00:11Z","timestamp":1774458011000},"page":"716-725","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":1,"title":["Insights From Mutational and Transcriptomic Profiles in Epithelial-myoepithelial Carcinoma"],"prefix":"10.1097","volume":"50","author":[{"given":"Ziyad","family":"Alsugair","sequence":"first","affiliation":[{"name":"Department of Pathology, Institut of Pathologie Multisite, Hospices Civils de Lyon, CHU Lyon Sud"},{"name":"Department of Biopathology, Centre Leon Berard"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fran\u00e7oise","family":"Descotes","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Hospices Civils de Lyon, CHU Lyon Sud"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne","family":"Champagnac","sequence":"additional","affiliation":[{"name":"Department of Biopathology, Centre Leon Berard"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonathan","family":"Lopez","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Hospices Civils de Lyon, CHU Lyon Sud"},{"name":"Universit\u00e9 Claude Bernard Lyon 1"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maxime","family":"Fieux","sequence":"additional","affiliation":[{"name":"Service of Oto-Rhino-Laryngology, CHU Lyon Sud, Hospices Civils de Lyon"},{"name":"Universit\u00e9 Claude Bernard Lyon 1"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pierre","family":"Philouse","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Claude Bernard Lyon 1"},{"name":"Service d\u2019Oto-Rhino-Laryngologie et Chirurgie Cervico-Faciale, H\u00f4pital La Croix Rousse, Hospices Civils de Lyon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philippe","family":"C\u00e9ruse","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Claude Bernard Lyon 1"},{"name":"Service d\u2019Oto-Rhino-Laryngologie et Chirurgie Cervico-Faciale, H\u00f4pital La Croix Rousse, Hospices Civils de Lyon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brice","family":"Thamphya","sequence":"additional","affiliation":[{"name":"Department of Biopathology, Centre Leon Berard"},{"name":"Team Genetics, Epigenetics and Biology of Sarcomas, Centre de Recherche en Canc\u00e9rologie de Lyon, INSERM U1052 - CNRS UMR5286, Centre L\u00e9on B\u00e9rard, Universit\u00e9 Claude Bernard Lyon 1, Lyon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Pissaloux","sequence":"additional","affiliation":[{"name":"Department of Biopathology, Centre Leon Berard"},{"name":"Team Genetics, Epigenetics and Biology of Sarcomas, Centre de Recherche en Canc\u00e9rologie de Lyon, INSERM U1052 - CNRS UMR5286, Centre L\u00e9on B\u00e9rard, Universit\u00e9 Claude Bernard Lyon 1, Lyon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Franck","family":"Tirode","sequence":"additional","affiliation":[{"name":"Department of Biopathology, Centre Leon Berard"},{"name":"Team Genetics, Epigenetics and Biology of Sarcomas, Centre de Recherche en Canc\u00e9rologie de Lyon, INSERM U1052 - CNRS UMR5286, Centre L\u00e9on B\u00e9rard, Universit\u00e9 Claude Bernard Lyon 1, Lyon, 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