{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T07:46:11Z","timestamp":1791963971637,"version":"4.3.4"},"reference-count":35,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"11","content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2026,11]]},"abstract":"<jats:p>\n                    ALK-negative anaplastic large cell lymphoma (ALK-negative ALCL) frequently expresses cytotoxic molecules, which may complicate its distinction from peripheral T-cell lymphoma NOS (PTCL-NOS), particularly in cytotoxic molecule-positive cases. Among these, nodal EBV-negative cytotoxic T-cell lymphoma (CTL) represents an important diagnostic consideration. We evaluated the clinicopathological features of ALK-negative ALCL in comparison with nodal EBV-negative CTL and ALK-positive ALCL, with a focus on the immunohistochemical expression of master transcription factors (ROR\u03b3t, T-bet, and GATA3). ROR\u03b3t expression was significantly more frequent in ALK-negative ALCL than in nodal EBV-negative CTL (11\/21 [52%] vs. 2\/25 [8%];\n                    <jats:italic toggle=\"yes\">P<\/jats:italic>\n                    =0.001), and was uniformly present in ALK-positive ALCL (10\/10 [100%];\n                    <jats:italic toggle=\"yes\">P<\/jats:italic>\n                    =0.012 vs. ALK-negative ALCL). In contrast, T-bet expression was less frequent in ALK-negative ALCL (2\/21 [10%]) than in nodal EBV-negative CTL (9\/25 [36%];\n                    <jats:italic toggle=\"yes\">P<\/jats:italic>\n                    =0.045), and was absent in all ALK-positive ALCL cases. ROR\u03b3t and T-bet expression were largely mutually exclusive across the cohort, with only one overlapping case. GATA3 expression was absent in all evaluable cases of nodal EBV-negative CTL and ALK-positive ALCL, and was detected in only 2 of 19 ALK-negative ALCL cases (11%). These findings suggest that ALK-negative ALCL shows a distinct transcription factor profile characterized by frequent ROR\u03b3t expression and infrequent T-bet expression, in contrast to nodal EBV-negative CTL. Assessment of these markers may provide a useful adjunct in the differential diagnosis of cytotoxic T-cell lymphomas, particularly in diagnostically challenging cases.\n                  <\/jats:p>","DOI":"10.1097\/pas.0000000000002602","type":"journal-article","created":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T08:00:53Z","timestamp":1788422453000},"page":"1307-1315","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":0,"title":["Differential Expression of ROR\u03b3t and T-bet in ALK-Negative Anaplastic Large Cell Lymphoma"],"prefix":"10.1097","volume":"50","author":[{"given":"Aya","family":"Muramatsu","sequence":"first","affiliation":[{"name":"Department of Pathology, Nagoya Ekisaikai Hospital"},{"name":"Center for Clinical Pathology, Fujita Health University Hospital, Toyoake"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daisuke","family":"Yamashita","sequence":"additional","affiliation":[{"name":"Department of Pathology, Kobe City Hospital Organization, Kobe City Medical Center General Hospital, Kobe"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuta","family":"Tsuyuki","sequence":"additional","affiliation":[{"name":"Center for Clinical Pathology, Fujita Health University Hospital, Toyoake"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akira","family":"Satou","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, Nagoya University Hospital"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naoko","family":"Asano","sequence":"additional","affiliation":[{"name":"Department of Molecular Diagnostics, Nagano Prefectural Shinshu Medical Center, Suzaka"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Waki","family":"Hosoda","sequence":"additional","affiliation":[{"name":"Department of Pathology and Molecular Diagnostics, Aichi Cancer Center Hospital, Nagoya"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kennosuke","family":"Karube","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, Nagoya University Hospital"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shigeo","family":"Nakamura","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, Nagoya University Hospital"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9713-3691","authenticated-orcid":false,"given":"Seiichi","family":"Kato","sequence":"additional","affiliation":[{"name":"Department of Pathology and Microbiology, Faculty of Medicine, Saga University, Saga, 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