{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T18:01:15Z","timestamp":1790186475365,"version":"4.1.0"},"reference-count":22,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"3","content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:sec>\n                    <jats:title>Objective:<\/jats:title>\n                    <jats:p>To evaluate the risk of red blood cell alloimmunization following transfusion of low-titer group O whole blood (LTOWB) compared with component therapy in severely injured trauma patients.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Summary Background Data:<\/jats:title>\n                    <jats:p>LTOWB is increasingly used for trauma resuscitation because of logistical advantages and potential hemostatic benefits. However, concerns remain regarding alloimmunization, particularly anti-D antibody formation in Rh(D)-negative patients exposed to Rh(D)-positive blood products.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>A retrospective cohort study was conducted at a single Level I trauma center using trauma registry data from 2017 to 2023. Adult trauma patients (15\u00a0y or older) who received emergency release uncrossed blood products prehospital or shortly after admission were included. Patients were categorized by exposure to Rh(D)-positive LTOWB (WB group) or Rh(D)-negative component therapy alone (COMP group). Antibody screening was performed on admission and repeated at 72-hour intervals during hospitalization. The primary outcome was the incidence of alloimmunization.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>A total of 3531 patients met the inclusion criteria, including 2103 in the WB group and 1428 in the COMP group. Post-transfusion alloantibody formation occurred in 3% of WB patients (n=63) and 2% of COMP patients (n=32). Among WB patients who developed alloantibodies, anti-D antibodies accounted for 11% of detected antibodies, with anti-E most common (22%). In the COMP cohort, anti-D antibodies accounted for 20% of the detected antibodies. In both groups, alloantibody formation was more common in Rh(D)-negative patients.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions:<\/jats:title>\n                    <jats:p>LTOWB transfusion in severely injured trauma patients was associated with a low incidence of alloantibody formation comparable to component therapy, supporting the immunohematological safety of LTOWB in trauma resuscitation.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1097\/sla.0000000000007137","type":"journal-article","created":{"date-parts":[[2026,6,22]],"date-time":"2026-06-22T12:00:13Z","timestamp":1782129613000},"page":"566-572","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":1,"title":["What is the Incidence of Early Antibody Formation After Resuscitation of Hemorrhage? A Comparison of RH(D)+ Whole Blood and RH(D)\u2212 Red Blood Cells in 3531 Trauma Patients"],"prefix":"10.1097","volume":"284","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-5030-8446","authenticated-orcid":false,"given":"Connor D.","family":"Purvis","sequence":"first","affiliation":[{"name":"Departments of Surgery and Pathology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Devi","family":"Bavishi","sequence":"additional","affiliation":[{"name":"Departments of Surgery and Pathology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammed","family":"Rigi","sequence":"additional","affiliation":[{"name":"Laboratory Medicine, McGovern Medical School, University of Texas Health Science Center"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandra P.","family":"Foster","sequence":"additional","affiliation":[{"name":"Departments of Surgery and Pathology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elene 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