{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T02:36:11Z","timestamp":1773801371820,"version":"3.50.1"},"reference-count":31,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"10","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2019,10]]},"abstract":"\n Background.<\/jats:title>\n Hypothermic ex situ perfusion (HESP) systems are used to prolong allograft survival in solid organ transplantations and have been shown to be superior to static cold storage (SCS) methods. However, the effect of this preservation method on limb allograft survival and long-term function has not yet been tested. In this study, we investigated the long-term effects of the HESP on skeletal muscle metabolism, structure, and force generation and compared it with the current standard of preservation.<\/jats:p>\n <\/jats:sec>\n \n Methods.<\/jats:title>\n Forty male Lewis rats (250 \u00b1 25\u2009g) were divided into 5 groups, including naive control, sciatic nerve transection or repair, immediate transplantation, SCS, and HESP. For the SCS group, limbs were preserved at 4\u00b0C for 6 hours. In the HESP group, limbs were continuously perfused with oxygenated histidine-tryptophan-ketoglutarate (HTK) solution at 10\u201315\u00b0C for 6 hours. Hemodynamic and biochemical parameters of perfusion were recorded throughout the experiment. At 12 weeks, electromyography and muscle force measurements (maximum twitch and tetanic forces) were obtained along with muscle samples for histology and metabolomics analysis.<\/jats:p>\n <\/jats:sec>\n \n Results.<\/jats:title>\n Histology demonstrated 48% myocyte injury in the HESP group compared with 49% in immediate transplantation (P<\/jats:italic> = 0.96) and 74% in the SCS groups (P<\/jats:italic> < 0.05). The maximum twitch force measurement revealed a significantly higher force in the HESP group compared with the SCS group (P<\/jats:italic> = 0.029). Essential amino acid levels of the gastrocnemius muscle did not reach significance, with the exception of higher proline levels in the HESP group.<\/jats:p>\n <\/jats:sec>\n \n Conclusions.<\/jats:title>\n HESP using HTK protects viability of the limb but fails to restore muscle force in the long term.<\/jats:p>\n <\/jats:sec>","DOI":"10.1097\/tp.0000000000002800","type":"journal-article","created":{"date-parts":[[2019,6,15]],"date-time":"2019-06-15T03:44:05Z","timestamp":1560570245000},"page":"2105-2112","source":"Crossref","is-referenced-by-count":10,"title":["Long-term Effects of Hypothermic Ex Situ Perfusion on Skeletal Muscle Metabolism, Structure, and Force Generation After Transplantation"],"prefix":"10.1097","volume":"103","author":[{"given":"Emre","family":"Gok","sequence":"first","affiliation":[{"name":"Department of Orthopaedic Surgery, University of Michigan Health System, Ann Arbor, MI."},{"name":"Department of Surgery, University of Michigan Health System, Ann Arbor, MI."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carrie A.","family":"Kubiak","sequence":"additional","affiliation":[{"name":"Department of Surgery, Division of Plastic Surgery, University of Michigan Health System, Ann Arbor, MI."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Erin","family":"Guy","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Surgery, University of Michigan Health System, Ann Arbor, MI."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mathew","family":"Ponder","sequence":"additional","affiliation":[{"name":"Department of Surgery, University of Michigan Health System, Ann Arbor, MI."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mark J.","family":"Hoenerhoff","sequence":"additional","affiliation":[{"name":"Unit for Laboratory Animal Medicine, University of Michigan, Ann Arbor, MI."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alvaro","family":"Rojas-Pena","sequence":"additional","affiliation":[{"name":"Department of Surgery, University of Michigan Health System, Ann Arbor, MI."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephen W.P.","family":"Kemp","sequence":"additional","affiliation":[{"name":"Department of Surgery, Division of Plastic Surgery, University of Michigan Health System, Ann Arbor, MI."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert H.","family":"Bartlett","sequence":"additional","affiliation":[{"name":"Department of Surgery, University of Michigan Health System, Ann Arbor, MI."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kagan","family":"Ozer","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Surgery, University of Michigan Health System, Ann Arbor, MI."},{"name":"Department of Surgery, Division of Plastic Surgery, University of Michigan 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