{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,31]],"date-time":"2025-12-31T07:08:17Z","timestamp":1767164897608,"version":"build-2238731810"},"reference-count":45,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"7","content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2021,7]]},"abstract":"<jats:sec>\n                    <jats:title>Background.<\/jats:title>\n                    <jats:p>Allogeneic hematopoietic stem cell transplantation (HSCT) is a potentially curative option for patients with hematologic diseases but is associated with high mortality and morbidity. Cytomegalovirus (CMV) infection is common in HSCT patients and modulates vitamin D metabolism in vitro. We aimed at validating CMV-associated vitamin D metabolism in vivo in HSCT.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods.<\/jats:title>\n                    <jats:p>Patients treated for significant CMV viremia after HSCT were evaluated for CMV load before, during, and after antiviral treatment. RNA was isolated from whole-blood samples to test for regulation of key components of the vitamin D receptor (VDR) pathway during different phases of CMV viremia.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results.<\/jats:title>\n                    <jats:p>\n                      CMV viremia developed a mean time of 102 (\u00b134) d post-HSCT. Maximum levels of CMV-DNA reached a mean of 5668 (\u00b17257) copies\/mL. VDR expression was downregulated to a mean of 64.3% (\u00b142.5%) relative to the VDR expression pre-CMV viremia (\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      = 0.035) and lagged in recovery following antiviral treatment. Toll-like receptor (TLR) 2 mRNA was upregulated to 225.4% during CMV viremia relative to the expression pre-CMV viremia (\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      = 0.012) but not TLR6\/7\/8 and the TLR-adaptor protein MyD88. Levels of 25-OH vitamin D were reduced in all viremic patients (48.0 \u00b1 4.8 versus 25.1 \u00b1 3.7 ng\/mL) and were even lower after periods of CMV viremia compared with the control group (48.3 \u00b1 3.5 versus 17.8 \u00b1 1.8 ng\/mL;\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      = 0.008).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions.<\/jats:title>\n                    <jats:p>CMV viremia is associated with significant dysregulation of vitamin D metabolism in HSCT patients.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1097\/tp.0000000000003448","type":"journal-article","created":{"date-parts":[[2020,9,4]],"date-time":"2020-09-04T20:00:15Z","timestamp":1599249615000},"page":"1595-1602","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":9,"title":["Cytomegalovirus Infection Downregulates Vitamin D Receptor in Patients Undergoing Hematopoietic Stem Cell Transplantation"],"prefix":"10.1097","volume":"105","author":[{"given":"Oliver","family":"Robak","sequence":"first","affiliation":[{"name":"Department of Internal Medicine I, Medical University of Vienna, Vienna, Austria."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marie-Theres","family":"Kastner","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine I, Medical University of Vienna, Vienna, Austria."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carmen","family":"Stecher","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine I, Medical University of Vienna, Vienna, Austria."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martina","family":"Schneider","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine I, Medical University of Vienna, Vienna, Austria."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martin","family":"Andreas","sequence":"additional","affiliation":[{"name":"Department of Cardiac Surgery, Medical University of Vienna, Vienna, Austria."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hildegard","family":"Greinix","sequence":"additional","affiliation":[{"name":"Department of Haematology, Medical University of Graz, Graz, Austria."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Enik\u00f6","family":"Kallay","sequence":"additional","affiliation":[{"name":"Department of Pathophysiology and Allergy Research, Medical University of Vienna, Vienna, Austria."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claudia","family":"Honsig","sequence":"additional","affiliation":[{"name":"Division of Clinical Virology, Department of Laboratory Medicine, Medical University of Vienna, Vienna, Austria."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christoph","family":"Steininger","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine I, Medical University of Vienna, Vienna, Austria."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"276","published-online":{"date-parts":[[2020,9,2]]},"reference":[{"key":"R1-20250604","doi-asserted-by":"crossref","first-page":"1472","DOI":"10.1056\/NEJMp078166","article-title":"Hematopoietic-cell transplantation at 50.","volume":"357","author":"Appelbaum","year":"2007","journal-title":"N Engl J Med"},{"key":"R2-20250604","doi-asserted-by":"crossref","first-page":"906","DOI":"10.1038\/bmt.2012.66","article-title":"The EBMT activity survey: 1990-2010.","volume":"47","author":"Passweg","year":"2012","journal-title":"Bone Marrow Transplant"},{"key":"R3-20250604","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1053\/bbmt.2002.v8.pm11846354","article-title":"A survey of diagnosis, management, and grading of chronic GVHD.","volume":"8","author":"Lee","year":"2002","journal-title":"Biol Blood Marrow Transplant"},{"key":"R4-20250604","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1056\/NEJM199907013410103","article-title":"Long-term survival and late deaths after allogeneic bone marrow transplantation. 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