{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,20]],"date-time":"2026-03-20T14:37:19Z","timestamp":1774017439983,"version":"3.50.1"},"reference-count":62,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"2","content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":["J Low Genit Tract Dis"],"published-print":{"date-parts":[[2021,4]]},"abstract":"<jats:sec>\n            <jats:title>Objectives<\/jats:title>\n            <jats:p>The aims of the study were to assess the available literature concerning the indications, performance, technique, and classification of wet mount microscopy (WMM) and to establish evidence-based recommendations.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Methods<\/jats:title>\n            <jats:p>Literature review from the main scientific databases was performed by the ad hoc \u201cVaginitis and Microbiome Committee\u201d of the International Society for the Study of Vulvovaginal Disease. The document was approved by the executive council and membership of the International Society for the Study of Vulvovaginal Disease.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>Available data are limited and usually of low level of evidence. Nevertheless, it shows that WMM is capable of reducing misdiagnosis, overtreatment, and undertreatment of vaginal conditions. It has an excellent performance for the diagnosis of bacterial vaginosis and variable performance for trichomoniasis and candidiasis. It is the gold standard for aerobic vaginitis\/desquamative inflammatory vaginitis. Currently, there is no recommendation to use WMM in the screening of asymptomatic women.<\/jats:p>\n            <jats:p>The use of phase contrast is recommended to improve performance and reproducibility. Sampling location, devices, and technique have an impact on the results.<\/jats:p>\n            <jats:p>Available scoring and classification scores have significant limitations.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusions<\/jats:title>\n            <jats:p>Wet mount microscopy is a point-of-care, inexpensive, and fast technique that, with practice, can be mastered by office clinicians. It should be considered a basic skill in the curricula of gynecology and obstetrics residencies. Recommendations are provided on sampling, reading, and scoring.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1097\/lgt.0000000000000595","type":"journal-article","created":{"date-parts":[[2021,2,27]],"date-time":"2021-02-27T07:09:58Z","timestamp":1614409798000},"page":"172-180","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":30,"title":["The International Society for the Study of Vulvovaginal Disease Vaginal Wet Mount Microscopy Guidelines: How to Perform, Applications, and Interpretation"],"prefix":"10.1097","volume":"25","author":[{"given":"Pedro","family":"Vieira-Baptista","sequence":"first","affiliation":[]},{"given":"\u0160vitrigail\u0117","family":"Grincevi\u010dien\u0117","sequence":"additional","affiliation":[{"name":"Department of Biothermodynamics and Drug Design, Institute of Biotechnology, Life Sciences Center, Vilnius University, Vilnius, Lithuania"}]},{"given":"Caroline","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Maternal Child Department, Fluminense Federal University, Niter\u00f3i, Rio de Janeiro, Brazil"}]},{"given":"Jos\u00e9","family":"Fonseca-Moutinho","sequence":"additional","affiliation":[{"name":"Health Sciences Research Centre, Faculty of Health Sciences, University of Beira Interior, Covilh\u00e3, Portugal"}]},{"given":"Facundo","family":"Cherey","sequence":"additional","affiliation":[{"name":"Lower Genital Tract Unit, Hospital de Cl\u00ednicas \u201cJos\u00e9 de San Mart\u00edn,\u201d Buenos Aires University, Buenos Aires, Argentina"}]},{"given":"Colleen Kennedy","family":"Stockdale","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, University of Iowa Hospitals and Clinics, Iowa City, IA"}]}],"member":"276","published-online":{"date-parts":[[2021,2,25]]},"reference":[{"key":"bib1-20250524","first-page":"ciaa260","article-title":"Diagnosis and 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