{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T01:32:06Z","timestamp":1772155926885,"version":"3.50.1"},"reference-count":33,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"4","content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":["J Low Genit Tract Dis"],"published-print":{"date-parts":[[2022,10]]},"abstract":"<jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>The etiology of localized provoked vulvodynia (LPV) remains unknown, but observations suggest the involvement of the vaginal microbiota. We examined the vaginal microbiota of women with LPV and healthy controls, upon after a low-oxalate diet (LOD).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods<\/jats:title>\n                    <jats:p>A total of 9 women diagnosed with secondary LPV and 21 healthy controls were recruited from the Galilee Medical Center in Israel and subjected to prospective evaluations of their vaginal microbiota. Total DNA was extracted from vaginal discharge samples provided before and after following LOD for 3 weeks and was then subjected to 16S sequencing. Data obtained were then used to evaluate \u03b1 and \u03b2 diversity, identify differentially abundant bacterial taxa in LPV, and determine their impact on the metabolism.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      These evaluations revealed decreased diversity in the vaginal microbiota of women with LPV and identified the\n                      <jats:italic toggle=\"yes\">Ochrobactrum<\/jats:italic>\n                      genus and\n                      <jats:italic toggle=\"yes\">Pseudomonadaceae<\/jats:italic>\n                      family as indicators for LPV. In addition, we identified 23 differentially expressed bacterial metabolic pathways between the LPV and control samples and revealed that LOD could induce changes in the \u03b2 diversity of LPV vaginal microbiomes, which was further supported by some degree of pain reduction in patients.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Localized provoked vulvodynia and LOD were associated with shifts in the vaginal microbiota. However, the impact of these changes on the development of LPV requires additional studies with a larger cohort.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1097\/lgt.0000000000000689","type":"journal-article","created":{"date-parts":[[2022,8,9]],"date-time":"2022-08-09T11:09:42Z","timestamp":1660043382000},"page":"339-344","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":7,"title":["Changes in the Vaginal Microbiota of Women With Secondary Localized Provoked Vulvodynia"],"prefix":"10.1097","volume":"26","author":[{"given":"Yaseen","family":"Awad-Igbaria","sequence":"first","affiliation":[]},{"given":"Eilam","family":"Palzur","sequence":"additional","affiliation":[{"name":"The Research Institute of Galilee Medical Center, Nahariya, Israel"}]},{"given":"Manal","family":"Nasser","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Galilee Medical Center, Nahariya, Israel"}]},{"given":"Pedro","family":"Vieira-Baptista","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Hospital Lus\u00edadas Lisboa, Porto, Portugal"}]},{"given":"Jacob","family":"Bornstein","sequence":"additional","affiliation":[]}],"member":"276","published-online":{"date-parts":[[2022,8,6]]},"reference":[{"key":"bib1-20250524","doi-asserted-by":"crossref","first-page":"745","DOI":"10.1097\/AOG.0000000000001359","article-title":"2015 ISSVD, ISSWSH and IPPS consensus terminology and classification of persistent vulvar pain and vulvodynia","volume":"127","year":"2016","journal-title":"Obstet Gynecol"},{"key":"bib2-20250524","doi-asserted-by":"crossref","first-page":"38.e1","DOI":"10.1016\/j.ajog.2015.02.002","article-title":"Identification of novel mechanisms involved in generating localized vulvodynia pain","volume":"213","year":"2015","journal-title":"Am J Obstet Gynecol"},{"key":"bib3-20250524","first-page":"101ra91","article-title":"Repeated vulvovaginal fungal infections cause persistent pain in a mouse model of vulvodynia","volume":"3","year":"2011","journal-title":"Sci Transl Med"},{"key":"bib4-20250524","doi-asserted-by":"crossref","first-page":"631","DOI":"10.2147\/IJWH.S113416","article-title":"Provoked vestibulodynia: Current perspectives","volume":"9","year":"2017","journal-title":"Int J Womens Health"},{"key":"bib6-20250524","first-page":"315","article-title":"Vulvodynia: a review of pathophysiological factors and treatment options","volume":"28","year":"2008","journal-title":"Basic Clin Med"},{"key":"bib7-20250524","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1016\/S0301-2115(02)00276-2","article-title":"Interleukin-1beta gene polymorphism in women with vulvar vestibulitis syndrome","volume":"107","year":"2003","journal-title":"Eur J Obstet Gynecol Reprod Biol"},{"key":"bib8-20250524","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1038\/nature11234","article-title":"Structure, function and diversity of the healthy human microbiome","volume":"486","year":"2012","journal-title":"Nature"},{"key":"bib9-20250524","doi-asserted-by":"crossref","first-page":"103696","DOI":"10.1016\/j.micpath.2019.103696","article-title":"Crosstalk between vaginal microbiome and female health: a review","volume":"136","year":"2019","journal-title":"Microb Pathog"},{"key":"bib10-20250524","doi-asserted-by":"crossref","first-page":"e1002478","DOI":"10.1371\/journal.pmed.1002478","article-title":"The vaginal microbiome and sexually transmitted infections are interlinked: consequences for treatment and prevention","volume":"14","year":"2017","journal-title":"PLoS Med"},{"key":"bib11-20250524","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3389\/fcimb.2018.00001","article-title":"Dysbiosis of the vaginal microbiota and higher vaginal kynurenine\/tryptophan ratio reveals an association with Chlamydia trachomatis genital infections","volume":"8","year":"2018","journal-title":"Front Cell Infect Microbiol"},{"key":"bib12-20250524","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1186\/s12934-020-01464-4","article-title":"Vaginal microbiota and the potential of Lactobacillus derivatives in maintaining vaginal health","volume":"19","year":"2020","journal-title":"Microb Cell Fact"},{"key":"bib13-20250524","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1097\/LGT.0000000000000645","article-title":"The vaginal microbiome: III. 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