{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,24]],"date-time":"2026-02-24T09:14:58Z","timestamp":1771924498698,"version":"3.50.1"},"reference-count":18,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2009,1,2]],"date-time":"2009-01-02T00:00:00Z","timestamp":1230854400000},"content-version":"vor","delay-in-days":367,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Laryngoscope"],"published-print":{"date-parts":[[2008,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:bold>Objectives\/Hypothesis:<\/jats:bold> To develop and characterize a new model of laryngeal inflammation by analyzing the presence of neurogenic peptides and expression of cyclooxygenases (COX) and cytokines in the mucosa.<\/jats:p><jats:p><jats:bold>Study Design:<\/jats:bold> Laryngitis induced by nasogastric intubation (NGI) was evaluated by histopathologic changes of the mucosa, alterations in calcitonin gene related peptide (CGRP) and substance P (SP) neuropeptides in sensory fibers, and COX\u20101,2, and cytokine (interleukin [IL]\u20101, IL\u20106, IL\u201010, tumor necrosis factor [TNF]\u2010\u03b1) expression in the laryngeal mucosa.<\/jats:p><jats:p><jats:bold>Methods:<\/jats:bold> Rats submitted to NGI for 1 to 5 weeks were compared with controls. Laryngeal sections were immunostained for stereologic analysis of SP and CGRP fiber density and number of mucosal cells expressing COX\u20102. Alterations in inflammatory mediators were evaluated by quantitative reverse\u2010transcriptase polymerase chain reaction.<\/jats:p><jats:p><jats:bold>Results:<\/jats:bold> NGI induced metaplasia of the epithelium and narrowing of the laryngeal lumen because of hypertrophy of laryngeal glandules and edema. An initial decrease in CGRP\u2010 and SP\u2010immunoreactive fibers in the laryngeal mucosa (1\u20133 wk) was reverted with time (5 wk). COX\u20102 expression in mucosal cells increased progressively, reaching a maximum level at 5 weeks, and was observed in mononuclear immune cells, which is indicative of a chronic inflammatory process. In regard to mRNA expression levels of inflammatory mediators, TNF\u2010\u03b1 was increased during the 5 week NGI, and IL\u201010 decreased during the 5 weeks,whereas IL\u20101\u03b2, IL\u20106, and COX\u20102 increased in the first 1 to 2 weeks and returned to baseline at 5 weeks.<\/jats:p><jats:p><jats:bold>Conclusions:<\/jats:bold> This NGI model results in laryngeal chronic inflammation without direct mechanical aggression of the mucosa and may contribute to the study of future therapeutic approaches to this pathology.<\/jats:p>","DOI":"10.1097\/mlg.0b013e3181492400","type":"journal-article","created":{"date-parts":[[2009,2,19]],"date-time":"2009-02-19T21:47:43Z","timestamp":1235080063000},"page":"78-86","source":"Crossref","is-referenced-by-count":15,"title":["A New Model of Laryngitis: Neuropeptide, Cyclooxygenase, and Cytokine Profile"],"prefix":"10.1002","volume":"118","author":[{"given":"Manuel","family":"Lima\u2010Rodrigues","sequence":"first","affiliation":[]},{"given":"Ana","family":"Valle\u2010Fernandes","sequence":"additional","affiliation":[]},{"given":"Nuno","family":"Lamas","sequence":"additional","affiliation":[]},{"given":"Andrea","family":"Cruz","sequence":"additional","affiliation":[]},{"given":"F\u00e1tima","family":"Baltazar","sequence":"additional","affiliation":[]},{"given":"Fernanda","family":"Milanezi","sequence":"additional","affiliation":[]},{"given":"Rui","family":"Nunes","sequence":"additional","affiliation":[]},{"given":"Rui M.","family":"Reis","sequence":"additional","affiliation":[]},{"given":"Jorge","family":"Pedrosa","sequence":"additional","affiliation":[]},{"given":"Ant\u00f3nio G.","family":"Castro","sequence":"additional","affiliation":[]},{"given":"Armando","family":"Almeida","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2009,1,2]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0347(200101)23:1<59::AID-HED9>3.0.CO;2-A"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1177\/000348948109000511"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbspin.2004.01.010"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1097\/00005537-200406000-00022"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9343(01)00859-2"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2818.1999.00457.x"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0306-3623(97)00078-5"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1289\/ehp.93101234"},{"key":"e_1_2_7_10_2","first-page":"560","article-title":"Chronic rhinosinusitis and neuropeptides","volume":"133","author":"Lacroix J","year":"2003","journal-title":"Swiss Med Wkly"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.20061"},{"key":"e_1_2_7_12_2","first-page":"33","article-title":"Neurogenic inflammation in human and rodent skin","volume":"16","author":"Schmelz M","year":"2001","journal-title":"News Physiol Sci"},{"key":"e_1_2_7_13_2","doi-asserted-by":"publisher","DOI":"10.1124\/jpet.102.032797"},{"key":"e_1_2_7_14_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10194-005-0153-6"},{"key":"e_1_2_7_15_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.15-07-05411.1995"},{"key":"e_1_2_7_16_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.2457950"},{"key":"e_1_2_7_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0301-0082(00)00063-0"},{"key":"e_1_2_7_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0306-4522(03)00497-4"},{"key":"e_1_2_7_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainresrev.2005.11.004"}],"container-title":["The Laryngoscope"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1097%2FMLG.0b013e3181492400","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1097\/MLG.0b013e3181492400","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,14]],"date-time":"2023-11-14T13:35:17Z","timestamp":1699968917000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1097\/MLG.0b013e3181492400"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,1]]},"references-count":18,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2008,1]]}},"alternative-id":["10.1097\/MLG.0b013e3181492400"],"URL":"https:\/\/doi.org\/10.1097\/mlg.0b013e3181492400","archive":["Portico"],"relation":{},"ISSN":["0023-852X","1531-4995"],"issn-type":[{"value":"0023-852X","type":"print"},{"value":"1531-4995","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,1]]}}}