{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,17]],"date-time":"2025-11-17T14:28:04Z","timestamp":1763389684196,"version":"3.41.0"},"reference-count":32,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"2","content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2023,2]]},"abstract":"<jats:p>Medullary Thyroid Carcinoma (MTC) is a tumor of the neuroendocrine system. In recent years, the need to assess the MTC diagnostic-related parameters has emerged with the aim to elucidate the mechanisms involved in this pathology. The objective of this study was to evaluate the role of Matrix Metalloproteinases (MMPs) 2 and 9, their tissue inhibitors of matrix metalloproteinases (TIMPs), S100 protein, and amyloid in the diagnostic of MTC. Thirty-two samples with MTC (72% women) were included in this cross-sectional study and divided by groups: T category 1 (T1)\u226420\u00a0mm and T category 2 (T2) 20 to 40\u00a0mm of tumor size. MMPs 2 and 9, TIMPs 2 and 1, S100 protein, and calcitonin in tissues were obtained by immunohistochemical techniques. The presence of amyloid in tissue sections was detected on Thioflavin T\u2013stained slides under fluorescent microscope. Percentage of positive cells (P) observed for MMP-2 was higher in those samples presenting T2 MTC with respect to those with T1 MTC (<jats:italic toggle=\"yes\">P<\/jats:italic>&lt;0.05). Moreover, P-MMP-2 showed a direct correlation with higher T category of MTC (Rho=0.439, <jats:italic toggle=\"yes\">P<\/jats:italic>\u2009&lt;\u20090.001), whereas P-MPP-9 was directly correlated with S100 protein and the intensity of calcitonin in tissues (Rho=0.419, <jats:italic toggle=\"yes\">P<\/jats:italic>=0.017; Rho=0.422, <jats:italic toggle=\"yes\">P<\/jats:italic>=0.016, respectively. Therefore, MMPs were directly correlated with some traditional biomarkers of MTC. In this regard, P-MMP-2 was more expressed in type 2 MTC. Combining the analysis of traditional and other useful biomarkers of MTC as MMPs 2 and 9 could be a useful strategy in the diagnostic of MTC.<\/jats:p>","DOI":"10.1097\/pai.0000000000001092","type":"journal-article","created":{"date-parts":[[2022,12,13]],"date-time":"2022-12-13T20:00:09Z","timestamp":1670961609000},"page":"121-127","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":2,"title":["Matrix Metalloproteinases 2 and 9 and Their Tissue Inhibitors in the Diagnostics of Medullary Thyroid Carcinoma"],"prefix":"10.1097","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0183-6902","authenticated-orcid":false,"given":"H\u00e9ctor","family":"V\u00e1zquez-Lorente","sequence":"first","affiliation":[{"name":"Department of Physiology, School of Pharmacy, Institute of Nutrition and Food Technology \u201cJose\u0301 Mataix,\u201d University of Granada, Granada, Spain"}]},{"given":"Du\u0161ko M.","family":"Dun\u0111erovi\u0107","sequence":"additional","affiliation":[{"name":"Institute of Pathology, Faculty of Medicine, University of Belgrade"}]},{"given":"Svetislav B.","family":"Tati\u0107","sequence":"additional","affiliation":[{"name":"Institute of Pathology, Faculty of Medicine, University of Belgrade"}]},{"given":"Sanja","family":"Radojevi\u0107-\u0160kodri\u0107","sequence":"additional","affiliation":[{"name":"Institute of Pathology, Faculty of Medicine, University of Belgrade"}]},{"given":"Cl\u00e1udio M.","family":"Gomes","sequence":"additional","affiliation":[{"name":"Institute of Biosystems & Integrative Sciences"},{"name":"Department of Chemistry and Biochemistry, Faculty of Sciences, University of Lisbon, Lisbon, Portugal"}]},{"given":"Ivan R.","family":"Paunovi\u0107","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, University of Belgrade"},{"name":"Center for Endocrine Surgery, Clinical Center of Serbia"}]},{"given":"Vesna","family":"Dragutinovi\u0107","sequence":"additional","affiliation":[{"name":"Institute of Chemistry in Medicine, Faculty of Medicine, University of Belgrade, Belgrade, Serbia"}]}],"member":"276","published-online":{"date-parts":[[2022,11,28]]},"reference":[{"key":"R1-20250525","doi-asserted-by":"crossref","first-page":"226","DOI":"10.2174\/1871530319666181220165350","article-title":"An unusual case of medullary thyroid carcinoma and a revision of current literature","volume":"19","author":"Cipri","year":"2019","journal-title":"Endocr Metab Immune Disord Drug Targets"},{"issue":"suppl 1","key":"R2-20250525","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1186\/s12902-019-0367-2","article-title":"Calcitonin negative medullary thyroid carcinoma: a challenging diagnosis or a medical dilemma?","volume":"19","author":"Gambardella","year":"2019","journal-title":"BMC Endocr 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