{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T19:00:53Z","timestamp":1778612453335,"version":"3.51.4"},"reference-count":13,"publisher":"The Royal Society","issue":"4","license":[{"start":{"date-parts":[[2009,5,22]],"date-time":"2009-05-22T00:00:00Z","timestamp":1242950400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/royalsociety.org\/journals\/ethics-policies\/data-sharing-mining\/"}],"content-domain":{"domain":["royalsocietypublishing.org"],"crossmark-restriction":true},"short-container-title":["Biol. Lett."],"published-print":{"date-parts":[[2009,8,23]]},"abstract":"<jats:p>Members of the Family Bufonidae, true toads, are famous for their endogenously synthesized cardioactive steroids that serve as defensive toxins. Evolution of resistance to these toxins is not understood. We sequenced a key region of the toxin's binding site in the Na<jats:sup>+<\/jats:sup>\/K<jats:sup>+<\/jats:sup>ATPase for relevant taxa representing Hyloidea (including bufonids), Ranoidea and Archaeobatrachia and tested for positive selection in a phylogenetic context. Bufonidae were distinct from other Hyloidea at 4\u20136 of 12 sites and, with one exception, had a homologous amino acid sequence.<jats:italic>Melanophryniscus stelzneri<\/jats:italic>had a distinct sequence, consistent with other independent evidence for a differentiated toxin. Tests within Bufonidae detected positive selection within the binding region, providing, to our knowledge, the first evidence of this type for positive selection within Amphibia. There was no evidence for positive selection on Bufonidae or<jats:italic>M. stelzneri<\/jats:italic>lineages. Sequence change in<jats:italic>Leptodactylus ocellatus<\/jats:italic>, a leptodactylid predator of Bufonidae, provides a molecular basis for predator resistance possibly associated with gene duplication.<\/jats:p>","DOI":"10.1098\/rsbl.2009.0281","type":"journal-article","created":{"date-parts":[[2009,5,23]],"date-time":"2009-05-23T00:49:11Z","timestamp":1243039751000},"page":"513-516","update-policy":"https:\/\/doi.org\/10.1098\/crossmark-policy","source":"Crossref","is-referenced-by-count":29,"title":["Positive Darwinian selection results in resistance to cardioactive toxins in true toads (Anura: Bufonidae)"],"prefix":"10.1098","volume":"5","author":[{"given":"David J.","family":"Moore","sequence":"first","affiliation":[{"name":"School of Botany and Zoology, The Australian National University, Canberra, ACT 0200, Australia"},{"name":"CSIRO Entomology, Black Mountain Laboratories, Clunies Ross Street, Acton, Canberra, ACT 2601, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Damien C. T.","family":"Halliday","sequence":"additional","affiliation":[{"name":"CSIRO Entomology, Black Mountain Laboratories, Clunies Ross Street, Acton, Canberra, ACT 2601, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David M.","family":"Rowell","sequence":"additional","affiliation":[{"name":"School of Botany and Zoology, The Australian National University, Canberra, ACT 0200, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anthony J.","family":"Robinson","sequence":"additional","affiliation":[{"name":"CSIRO Entomology, Black Mountain Laboratories, Clunies Ross Street, Acton, Canberra, ACT 2601, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. Scott","family":"Keogh","sequence":"additional","affiliation":[{"name":"School of Botany and Zoology, The Australian National University, Canberra, ACT 0200, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"175","published-online":{"date-parts":[[2009,5,22]]},"reference":[{"key":"e_1_3_3_2_1","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1016\/S0022-3565(25)08042-5","article-title":"Relative susceptibility of the nebulous toad (Bufo valliceps) and the leopard frog (Rana pipiens) to different substances","volume":"47","author":"Chen K. K.","year":"1933","journal-title":"J. Pharmacol. Exp. Ther."},{"key":"e_1_3_3_3_1","first-page":"192","article-title":"Effects of Bufo (Anura: Bufonidae) toxins on tadpoles from native and exotic Bufo habitats","volume":"55","author":"Crossland M. 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