{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,30]],"date-time":"2026-03-30T17:17:41Z","timestamp":1774891061891,"version":"3.50.1"},"reference-count":15,"publisher":"The Royal Society","issue":"1","license":[{"start":{"date-parts":[[2019,1,1]],"date-time":"2019-01-01T00:00:00Z","timestamp":1546300800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/royalsociety.org\/journals\/ethics-policies\/data-sharing-mining\/"}],"funder":[{"DOI":"10.13039\/501100000522","name":"Company of Biologists","doi-asserted-by":"crossref","award":["JEB-171103"],"award-info":[{"award-number":["JEB-171103"]}],"id":[{"id":"10.13039\/501100000522","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["royalsocietypublishing.org"],"crossmark-restriction":true},"short-container-title":["Biol. Lett."],"published-print":{"date-parts":[[2019,1]]},"abstract":"<jats:p>Oxygen- and capacity-limited thermal tolerance (OCLTT) is a controversial hypothesis claiming to explain variation in, and mechanistically determine, animal thermal limits. The lack of support from Insecta is typically argued to be a consequence of their high-performance respiratory systems. However, no studies have reported internal body oxygen levels during thermal ramping so it is unclear if changes in ambient gas are partially or fully offset by a compensatory respiratory system. Here we provide such an assessment by simultaneously recording haemolymph oxygen (pO<jats:sub>2<\/jats:sub>) levels\u2014as an approximation of tissue oxygenation\u2014while experimentally manipulating ambient oxygen and subjecting organisms to thermal extremes in a series of thermolimit respirometry experiments using pupae of the butterfly<jats:italic>Pieris napi<\/jats:italic>. The main results are that while<jats:italic>P. napi<\/jats:italic>undergo large changes in haemolymph pO<jats:sub>2<\/jats:sub>that are positively correlated with experimental oxygen levels, haemolymph pO<jats:sub>2<\/jats:sub>is similar pre- and post-death during thermal assays. OCLTT predicts that reduction in body oxygen level should lead to a reduction in CTmax. Despite finding the former, there was no change in CTmax across a wide range of body oxygen levels. Thus, we argue that oxygen availability is not a functional determinant of the upper thermal limits in pupae of<jats:italic>P. napi<\/jats:italic>.<\/jats:p>","DOI":"10.1098\/rsbl.2018.0701","type":"journal-article","created":{"date-parts":[[2019,1,16]],"date-time":"2019-01-16T08:04:40Z","timestamp":1547625880000},"page":"20180701","update-policy":"https:\/\/doi.org\/10.1098\/crossmark-policy","source":"Crossref","is-referenced-by-count":15,"title":["Oxygen limitation is not the cause of death during lethal heat exposure in an insect"],"prefix":"10.1098","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8344-6830","authenticated-orcid":true,"given":"Philipp","family":"Lehmann","sequence":"first","affiliation":[{"name":"Department of Zoology, Stockholm University, Stockholm, 10691, Sweden"},{"name":"Centre for Invasion Biology, Department of Conservation Ecology and Entomology, Stellenbosch University, Stellenbosch, 7602, South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7878-2936","authenticated-orcid":true,"given":"Marion","family":"Javal","sequence":"additional","affiliation":[{"name":"Centre for Invasion Biology, Department of Conservation Ecology and Entomology, Stellenbosch University, Stellenbosch, 7602, South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9665-9405","authenticated-orcid":true,"given":"John S.","family":"Terblanche","sequence":"additional","affiliation":[{"name":"Centre for Invasion Biology, Department of Conservation Ecology and Entomology, Stellenbosch University, Stellenbosch, 7602, South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"175","published-online":{"date-parts":[[2019,1,16]]},"reference":[{"key":"e_1_3_6_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/s001140100216"},{"key":"e_1_3_6_3_2","doi-asserted-by":"publisher","DOI":"10.1242\/jeb.01023"},{"key":"e_1_3_6_4_2","doi-asserted-by":"publisher","DOI":"10.1242\/jeb.040170"},{"key":"e_1_3_6_5_2","doi-asserted-by":"publisher","DOI":"10.1086\/669932"},{"key":"e_1_3_6_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpa.2015.10.020"},{"key":"e_1_3_6_7_2","doi-asserted-by":"publisher","DOI":"10.1242\/jeb.130039"},{"key":"e_1_3_6_8_2","doi-asserted-by":"crossref","first-page":"2359","DOI":"10.1242\/jeb.201.16.2359","article-title":"Augmented respiration in a flying insect","volume":"201","author":"Komai Y","year":"1998","journal-title":"J. Exp. Biol."},{"key":"e_1_3_6_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-1910(00)00153-0"},{"key":"e_1_3_6_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature03106"},{"key":"e_1_3_6_11_2","doi-asserted-by":"publisher","DOI":"10.1111\/phen.12188"},{"key":"e_1_3_6_12_2","doi-asserted-by":"publisher","DOI":"10.1242\/jeb.120261"},{"key":"e_1_3_6_13_2","doi-asserted-by":"crossref","first-page":"345","DOI":"10.1242\/jeb.41.2.345","article-title":"Factors altering spiracle control in adult dragonflies: hypoxia and temperature","volume":"41","author":"Miller PL","year":"1964","journal-title":"J. Exp. Biol."},{"key":"e_1_3_6_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtherbio.2017.08.009"},{"key":"e_1_3_6_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpa.2007.08.017"},{"key":"e_1_3_6_16_2","unstructured":"Lehmann P Javal M Terblanche JS. 2019 Data from: Oxygen limitation is not the cause of death during lethal heat exposure in an insect. 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(doi:10.5061\/dryad.18qv7ks)"}],"container-title":["Biology Letters"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/royalsocietypublishing.org\/doi\/pdf\/10.1098\/rsbl.2018.0701","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/royalsocietypublishing.org\/doi\/full-xml\/10.1098\/rsbl.2018.0701","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/royalsocietypublishing.org\/doi\/pdf\/10.1098\/rsbl.2018.0701","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,9,10]],"date-time":"2022-09-10T10:08:39Z","timestamp":1662804519000},"score":1,"resource":{"primary":{"URL":"https:\/\/royalsocietypublishing.org\/doi\/10.1098\/rsbl.2018.0701"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,1]]},"references-count":15,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2019,1]]}},"alternative-id":["10.1098\/rsbl.2018.0701"],"URL":"https:\/\/doi.org\/10.1098\/rsbl.2018.0701","relation":{},"ISSN":["1744-9561","1744-957X"],"issn-type":[{"value":"1744-9561","type":"print"},{"value":"1744-957X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,1]]},"assertion":[{"value":"2018-10-03","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-12-18","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-01-16","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}