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However, unlike the flow-through cardiovascular system of vertebrates, insect tissues exchange gases through blind-ended tracheoles, which may involve a more prominent role for diffusive gas exchange. We tested the effect of progressive hypoxia on ventilation and the PO2 of the metathoracic ganglion (neural site of control of ventilation) using microelectrodes in the American locust, Schistocerca americana. In normal air (21 kPa), PO2 of the metathoracic ganglion was 12 kPa. The PO2 of the ganglion dropped as air PO2 dropped, with ventilatory responses occurring when ganglion PO2 reached 3 kPa. Unlike vertebrates, insects tolerate relatively high resting tissue PO2 levels and allow tissue PO2 to drop during hypoxia, activity and discontinuous gas exchange before activating convective or spiracular gas exchange. Tracheated animals, and possibly pancrustaceans in general, seem likely to generally experience wide spatial and temporal variation in tissue PO2 compared with vertebrates, with important implications for physiological function and the evolution of oxygen-using proteins.<\/jats:p>","DOI":"10.1098\/rsbl.2020.0548","type":"journal-article","created":{"date-parts":[[2020,11,3]],"date-time":"2020-11-03T19:08:50Z","timestamp":1604430530000},"update-policy":"https:\/\/doi.org\/10.1098\/crossmark-policy","source":"Crossref","is-referenced-by-count":8,"title":["<i>P<\/i>\n                    O2 of the metathoracic ganglion in response to progressive hypoxia in an insect"],"prefix":"10.1098","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5223-216X","authenticated-orcid":true,"given":"Jon F.","family":"Harrison","sequence":"first","affiliation":[{"name":"Tempe, AZ 85287-4501, USA 1","department":["School of Life Sciences, Arizona State University,"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8127-7674","authenticated-orcid":true,"given":"Wolfgang","family":"Waser","sequence":"additional","affiliation":[{"name":"Berlin, Philippstrasse 13, 10115 Berlin, Germany 2","department":["Animal Physiology and Systems Neurobiology, Humboldt University,"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4928-8506","authenticated-orcid":true,"given":"Stefan K.","family":"Hetz","sequence":"additional","affiliation":[{"name":"Berlin, Philippstrasse 13, 10115 Berlin, Germany 2","department":["Animal Physiology and Systems Neurobiology, Humboldt University,"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"175","published-online":{"date-parts":[[2020,11,4]]},"reference":[{"key":"2026010519345726800_RSBL20200548C1","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1016\/S0005-2728(98)00076-0","article-title":"Mitochondrial respiration in the low oxygen environment of the cell effect of ADP on oxygen kinetics","volume":"1365","author":"Gnaiger","year":"1998","journal-title":"Biochim. 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