{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T13:17:35Z","timestamp":1787318255970,"version":"build-2736575974"},"reference-count":38,"publisher":"The Royal Society","issue":"10","license":[{"start":{"date-parts":[[2023,10,1]],"date-time":"2023-10-01T00:00:00Z","timestamp":1696118400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/royalsociety.org\/journals\/ethics-policies\/data-sharing-mining\/"}],"funder":[{"DOI":"10.13039\/501100001509","name":"Royal Society Te Ap\u0101rangi","doi-asserted-by":"crossref","award":["14-UOA-210"],"award-info":[{"award-number":["14-UOA-210"]}],"id":[{"id":"10.13039\/501100001509","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["royalsocietypublishing.org"],"crossmark-restriction":true},"short-container-title":["Biol. Lett."],"published-print":{"date-parts":[[2023,10]]},"abstract":"<jats:p>\n                    Anoxia\/re-oxygenation (AR) results in elevated unchecked oxidative stress and mediates irreversible damage within the brain for most vertebrates. Succinate accumulation within mitochondria of the ischaemic brain appears to increase the production of reactive oxygen species (ROS) upon re-oxygenation. Two closely related elasmobranchs, the epaulette shark (\n                    <jats:italic>Hemiscyllium ocellatum<\/jats:italic>\n                    ) and the grey carpet shark (\n                    <jats:italic>Chiloscyllium punctatum<\/jats:italic>\n                    ) repeatedly experience near anoxia and re-oxygenation in their habitats and have adapted to survive AR at tropical temperatures without significant brain injuries. However, these anoxia-tolerant species display contrasting strategies to survive AR, with only\n                    <jats:italic>H. ocellatum<\/jats:italic>\n                    having the capacity to supress metabolism and\n                    <jats:italic>H. ocellatum<\/jats:italic>\n                    mitochondria the capacity to depress succinate oxidation post-AR. We measured oxygen consumption alongside ROS production mediated by elevated succinate in mitochondria of permeabilized cerebellum from both shark species. Although mitochondrial respiration remained similar for both species, the ROS production in\n                    <jats:italic>H. ocellatum<\/jats:italic>\n                    was half that of\n                    <jats:italic>C. punctatum<\/jats:italic>\n                    in phosphorylating and non-phosphorylating mitochondria. Maximum ROS production in\n                    <jats:italic>H. ocellatum<\/jats:italic>\n                    was mediated by succinate loads 10-fold higher than in\n                    <jats:italic>C. punctatum<\/jats:italic>\n                    mitochondria. The contrasting survival strategies of anoxia-tolerant sharks reveal the significance of mitigating ROS production under elevated succinate load during AR, shedding light on potential mechanisms to mitigate brain injury.\n                  <\/jats:p>","DOI":"10.1098\/rsbl.2023.0344","type":"journal-article","created":{"date-parts":[[2023,10,11]],"date-time":"2023-10-11T03:07:02Z","timestamp":1696993622000},"update-policy":"https:\/\/doi.org\/10.1098\/crossmark-policy","source":"Crossref","is-referenced-by-count":3,"title":["Succinate-mediated reactive oxygen species production in the anoxia-tolerant epaulette (\n                    <i>Hemiscyllium ocellatum<\/i>\n                    ) and grey carpet (\n                    <i>Chiloscyllium punctatum<\/i>\n                    ) sharks"],"prefix":"10.1098","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5280-3871","authenticated-orcid":true,"given":"Jules B. 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J. Exp. Biol. 219, 1130-1138.","journal-title":"J. Exp. Biol."},{"key":"e_1_3_8_17_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10695-010-9439-y"},{"key":"e_1_3_8_18_2","doi-asserted-by":"publisher","DOI":"10.1002\/jez.539"},{"key":"e_1_3_8_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-3940(00)01321-5"},{"key":"e_1_3_8_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpa.2008.03.022"},{"key":"e_1_3_8_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1095-6433(01)00484-6"},{"key":"e_1_3_8_22_2","doi-asserted-by":"publisher","DOI":"10.1242\/jeb.191353"},{"key":"e_1_3_8_23_2","unstructured":"Devaux J. 2023 Succinate-mediated ROS production in the anoxia-tolerant epaulette ( Hemiscyllium ocellatum ) and grey carpet ( Chiloscyllium punctatum ) sharks. The University of Auckland. Dataset. 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